BBa_K781002 1 BBa_K781002 [R0010][B0034] - mgfp-5-FliC Insertion Chimera 2012-10-01T11:00:00Z 2015-05-08T01:13:18Z This was created using PCR Overlap extension from K781000 Released HQ 2013 This is an mgfp-5 chimeric insertion flagella. false false _1035_ 0 9715 9 It's complicated false The linker and D3 overlap sequences are standard for our parts. By digesting the plasmid with the two inner enzymes, SpeI and NheI for our BB-2 standard parts, you can purify and then PCR overlap extend using the following protocol to result in a brand new chimera. false Kevin Chen annotation2329240 1 BBa_B0034 range2329240 1 209 220 annotation2329237 1 -10 range2329237 1 161 166 annotation2329239 1 start range2329239 1 173 173 annotation2329234 1 BBa_R0010 range2329234 1 1 200 annotation2329242 1 Flagellin range2329242 1 230 976 annotation2329246 1 Flagellin range2329246 1 1246 1996 annotation2329238 1 LacI binding site range2329238 1 166 200 annotation2329244 1 K197018 range2329244 1 998 1225 annotation2329235 1 CAP binding site range2329235 1 89 126 annotation2329245 1 Linker 2 range2329245 1 1226 1245 annotation2329236 1 -35 range2329236 1 137 142 annotation2329243 1 Linker 1 range2329243 1 977 997 annotation2329233 1 end of LacI coding region (inactive) range2329233 1 1 88 annotation2329241 1 conserved range2329241 1 213 216 BBa_K781002_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaaatactagagatggcacaagtcattaataccaacagcctctcgctgatcactcaaaataatatcaacaagaaccagtctgcgctgtcgagttctatcgagcgtctgtcttctggcttgcgtattaacagcgcgaaggatgacgcagcgggtcaggcgattgctaaccgtttcacctctaacattaaaggcctgactcaggcggcccgtaacgccaacgacggtatctccgttgcgcagaccaccgaaggcgcgctgtccgaaatcaacaacaacttacagcgtgtgcgtgaactgacggtacaggccactaccggtactaactctgagtctgatctgtcttctatccaggacgaaattaaatcccgtctggatgaaattgaccgcgtatctggtcagacccagttcaacggcgtgaacgtgctggcaaaaaatggctccatgaaaatccaggttggcgcaaatgataaccagactatcactatcgatctgaagcagattgatgctaaaactcttggccttgatggttttagcgttaaaaataacgatacagttaccacaagtgctccagtaactgcttttggtgctaccaccacaaacaatattaaacttactggaattaccctttctacggaagcagccactgatactggcggaactaacccagcttcaattgagggtgtttatactgataatggtaatgattactatgcgaaaatcaccggtggtgataacgatgggaagtattacgcagtaacaaccacaggaggtggaggttcatcttccgaggaatacaaaggtggttactacccgggtaacacctaccactaccactctggtggttcttaccacggttctggttaccacggtggttacaaaggtaaatactacggtaaagctaaaaaatactactacaaatacaaaaactctggtaaatacaaatacctgaaaaaagctcgtaaataccaccgtaaaggttacaaaaaatactacggtggtggttcttctggtggatcaggtggaacttcaacagtgacaatggcgactggagcaacggcaaatgcacatcatcaccatcaccacactgtaactgatgcaaatactactaaagctacaactatcacttcaggcggtacacctgttcagattgataatacagcaggttccgcaactgccaaccttggtgctgttagcttagtaaaattgcaggattccaagggtaatgataccgatacatatgcgcttaaagatacaaatggcaatctttacgctgcggatgtgaatgaaactactggtgctgtttctgttaaaactattacctatactgactcttccggtgccgccagttctccaaccgcggtcaaactgggcggagatgatggcaaaacagaagtggtcgatattgatggtaaaacatacgattctgccgatttaaatggcggtaatctgcaaacaggtttgactgctggtggtgaggctctgactgctgttgcaaatggtaaaaccacggatccgctgaaagcgctggacgatgctatcgcatctgtagacaaattccgttcttccctcggtgcggtgcaaaaccgtctggattccgcggttaccaacctgaacaacaccactaccaacctgtctgaagcgcagtcccgtattcaggacgccgactatgcgaccgaagtgtccaatatgtcgaaagcgcagatcatccagcaggccggtaactccgtgttggcaaaagctaaccaggtaccgcagcaggttctgtctctgttgcagggttaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z