LOCUS       BBa_K784047             2729 bp    DNA     linear       10-May-2024
DEFINITION  Theophylline riboswitch + wt T7 RNAP
ACCESSION   BBa_K784047
VERSION     BBa_K784047.1  
FEATURES             Location/Qualifiers
     misc_feature    1..17
                     /label=Additional 5' UTR
     misc_feature    18..55
                     /label=Theophylline aptamer
     RBS             56..74
                     /label=RBS
     misc_feature    1..74
                     /label=BBa_K784005
     promoter        2572..2599
                     /label=putative E. coli promoter
     CDS             75..2729
                     /label=BBa_I2032
ORIGIN
        1 gactcactat aggtaccggt gataccagca tcgtcttgat gcccttggca gcaccctgct
       61 aaggtaacaa caagatgaac acgattaaca tcgctaagaa cgacttctct gacatcgaac
      121 tggctgctat cccgttcaac actctggctg accattacgg tgagcgttta gctcgcgaac
      181 agttggccct tgagcatgag tcttacgaga tgggtgaagc acgcttccgc aagatgtttg
      241 agcgtcaact taaagctggt gaggttgcgg ataacgctgc cgccaagcct ctcatcacta
      301 ccctactccc taagatgatt gcacgcatca acgactggtt tgaggaagtg aaagctaagc
      361 gcggcaagcg cccgacagcc ttccagttcc tgcaagaaat caagccggaa gccgtagcgt
      421 acatcaccat taagaccact ctggcttgcc taaccagtgc tgacaataca accgttcagg
      481 ctgtagcaag cgcaatcggt cgggccattg aggacgaggc tcgcttcggt cgtatccgtg
      541 accttgaagc taagcacttc aagaaaaacg ttgaggaaca actcaacaag cgcgtagggc
      601 acgtctacaa gaaagcattt atgcaagttg tcgaggctga catgctctct aagggtctac
      661 tcggtggcga ggcgtggtct tcgtggcata aggaagactc tattcatgta ggagtacgct
      721 gcatcgagat gctcattgag tcaaccggaa tggttagctt acaccgccaa aatgctggcg
      781 tagtaggtca agactctgag actatcgaac tcgcacctga atacgctgag gctatcgcaa
      841 cccgtgcagg tgcgctggct ggcatctctc cgatgttcca accttgcgta gttcctccta
      901 agccgtggac tggcattact ggtggtggct attgggctaa cggtcgtcgt cctctggcgc
      961 tggtgcgtac tcacagtaag aaagcactga tgcgctacga agacgtttac atgcctgagg
     1021 tgtacaaagc gattaacatt gcgcaaaaca ccgcatggaa aatcaacaag aaagtcctag
     1081 cggtcgccaa cgtaatcacc aagtggaagc attgtccggt cgaggacatc cctgcgattg
     1141 agcgtgaaga actcccgatg aaaccggaag acatcgacat gaatcctgag gctctcaccg
     1201 cgtggaaacg tgctgccgct gctgtgtacc gcaaggacaa ggctcgcaag tctcgccgta
     1261 tcagccttga gttcatgctt gagcaagcca ataagtttgc taaccataag gccatctggt
     1321 tcccttacaa catggactgg cgcggtcgtg tttacgctgt gtcaatgttc aacccgcaag
     1381 gtaacgatat gaccaaagga ctgcttacgc tggcgaaagg taaaccaatc ggtaaggaag
     1441 gttactactg gctgaaaatc cacggtgcaa actgtgcggg tgtcgataag gttccgttcc
     1501 ctgagcgcat caagttcatt gaggaaaacc acgagaacat catggcttgc gctaagtctc
     1561 cactggagaa cacttggtgg gctgagcaag attctccgtt ctgcttcctt gcgttctgct
     1621 ttgagtacgc tggggtacag caccacggcc tgagctataa ctgctccctt ccgctggcgt
     1681 ttgacgggtc ttgctctggc atccagcact tctccgcgat gctccgagat gaggtaggtg
     1741 gtcgcgcggt taacttgctt cctagtgaaa ccgttcagga catctacggg attgttgcta
     1801 agaaagtcaa cgagattcta caagcagacg caatcaatgg gaccgataac gaagtagtta
     1861 ccgtgaccga tgagaacact ggtgaaatct ctgagaaagt caagctgggc actaaggcac
     1921 tggctggtca atggctggct tacggtgtta ctcgcagtgt gactaagcgt tcagtcatga
     1981 cgctggctta cgggtccaaa gagttcggct tccgtcaaca agtgctggaa gataccattc
     2041 agccagctat tgattccggc aagggtctga tgttcactca gccgaatcag gctgctggat
     2101 acatggctaa gctgatttgg gaatctgtga gcgtgacggt ggtagctgcg gttgaagcaa
     2161 tgaactggct taagtctgct gctaagctgc tggctgctga ggtcaaagat aagaagactg
     2221 gagagattct tcgcaagcgt tgcgctgtgc attgggtaac tcctgatggt ttccctgtgt
     2281 ggcaggaata caagaagcct attcagacgc gcttgaacct gatgttcctc ggtcagttcc
     2341 gcttacagcc taccattaac accaacaaag atagcgagat tgatgcacac aaacaggagt
     2401 ctggtatcgc tcctaacttt gtacacagcc aagacggtag ccaccttcgt aagactgtag
     2461 tgtgggcaca cgagaagtac ggaatcgaat cttttgcact gattcacgac tccttcggta
     2521 ccattccggc tgacgctgcg aacctgttca aagcagtgcg cgaaactatg gttgacacat
     2581 atgagtcttg tgatgtactg gctgatttct acgaccagtt cgctgaccag ttgcacgagt
     2641 ctcaattgga caaaatgcca gcacttccgg ctaaaggtaa cttgaacctc cgtgacatct
     2701 tagagtcgga cttcgcgttc gcgtaataa
//