Polyargini

BBa_K792004 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K792004
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Manuel Gim??nez
Date created: 2012-09-23 11:00:00
Date modified: 2015-05-08 01:13:23

Import enhancer - Polyarginine (trojan peptide)



Types
DnaRegion

Roles
Other

sequence_feature

Sequences BBa_K792004_sequence (Version 1)

Description

Trojan peptides are short sequences that penetrate through the plasma membrane inside the cell without the need of any receptor or endocitosis process [Derossi 1998]. They can be used to increase the efficiency with which a protein enters a cell.

This part is Polyarginine, and has to be used just before the coding sequence of the peptide that you want to be import enhanced.

(refer to Characterisation of cell-penetrating peptide-mediated peptide delivery [Jones et al 2005] for more information about polyarginine)

Notes

Sequence obtained by retro-translation. Codon and mRNA-secondary structure optimized for yeast.

Source

Characterisation of cell-penetrating peptide-mediated peptide delivery [Jones et al 2005]

Sequence Annotation Location Component / Role(s)
HindIII restriction site
Trojan peptide
1,6
7,39
sequence_feature feature/misc
CDS feature/cds
igem#experience
None
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K792004/1