BBa_K416003 1 BBa_K416003 Yeast Secretion Tag 2010-06-22T11:00:00Z 2015-05-08T01:12:27Z This part was sent to use from Dr. Sheldon Park of the University of Buffalo. This secretion tag when linked to the N-terminal of the protein directs extracellular secretion of the protein. After targeting through the ER to the golgi, this "pre-pro" tag is cleaved by the KEX-2 protease resulting in secretion of protein without the aforementioned tag. To use this tag, attached it directly upstream of your proteins CDS. false false _527_ 0 7430 9 It's complicated true There was an illegal Xbal1 site at bp 101 and we removed it through altering the primers necessary for biobricking the sequence. false John Phair BBa_K792013 1 BamHI rs BamHI restriction site (standalone) 2012-09-24T11:00:00Z 2015-05-08T01:13:23Z None This is a '''BamHI'''restriction site. This part might not be useful as a physical biobrick. It has been created to follow modularization in parts X false false _1047_ 0 11811 9 Not in stock false None false Manuel Gim??nez annotation2197287 1 BamHI restriction site range2197287 1 1 6 BBa_J63003 1 Kozak & st designed yeast Kozak sequence 2006-10-10T11:00:00Z 2015-08-31T01:56:26Z consensus Kozak and start built from oligonucleotides Released HQ 2013 consensus Kozak sequence and start codon false false _97_ 0 545 97 In stock false designed such that fusion using fusion bricks assembly method leads to in frame translation true Caroline Ajo-Franklin annotation1902852 1 start codon range1902852 1 13 15 BBa_K792007 1 PolyHb Histidine rich peptide (PolyHb) 2012-09-24T11:00:00Z 2015-05-08T01:13:23Z Designed by BsAs Team 2012 Stable and soluble Histidine rich peptide. This part has a '''HindIII''' restriction site as extra. false false _1047_ 0 11811 9 Not in stock false PolyHb was desing taking into acount the following consideration: # avoided repeating the same residue in tandem to minimize local tRNA depleation # avoided Trp in tandem because of therir low solubility # we included Gly to avoid the formation of stable structures # included acidic and basic amino acids to increase solubility Sequence obtained by retro-translation. Codon and mRNA-secondary structure optimized for yeast. false Manuel Gim??nez annotation2196617 1 stop range2196617 1 115 120 annotation2196465 1 HindIII restriction site range2196465 1 1 6 annotation2204263 1 cds range2204263 1 7 114 BBa_K792011 1 BBa_K792011 Yeast exportable His-rich peptide w/enhanced import (2) 2012-09-24T11:00:00Z 2015-05-08T01:13:23Z Composite device Pato false false _1047_ 0 11811 9 It's complicated false Composite device false Manuel Gim??nez component2197299 1 BBa_K792013 component2197301 1 BBa_J63003 component2197302 1 BBa_K416003 component2197307 1 BBa_K792007 component2197304 1 BBa_K792004 annotation2197302 1 BBa_K416003 range2197302 1 25 138 annotation2197301 1 BBa_J63003 range2197301 1 7 24 annotation2197299 1 BBa_K792013 range2197299 1 1 6 annotation2197304 1 BBa_K792004 range2197304 1 139 177 annotation2197307 1 BBa_K792007 range2197307 1 178 297 BBa_K792004 1 Polyargini Import enhancer - Polyarginine (trojan peptide) 2012-09-23T11:00:00Z 2015-05-08T01:13:23Z Characterisation of cell-penetrating peptide-mediated peptide delivery [Jones et al 2005] Trojan peptides are short sequences that penetrate through the plasma membrane inside the cell without the need of any receptor or endocitosis process [Derossi 1998]. They can be used to increase the efficiency with which a protein enters a cell. This part is Polyarginine, and has to be used just before the coding sequence of the peptide that you want to be import enhanced. (refer to ''Characterisation of cell-penetrating peptide-mediated peptide delivery'' [Jones et al 2005] for more information about polyarginine) false false _1047_ 0 11811 9 Not in stock false Sequence obtained by retro-translation. Codon and mRNA-secondary structure optimized for yeast. false Manuel Gim??nez annotation2196615 1 HindIII restriction site range2196615 1 1 6 annotation2197880 1 Trojan peptide range2197880 1 7 39 BBa_K792004_sequence 1 aagcttagaagaagaagaagaagaagacgtcgtcgtaga BBa_K792007_sequence 1 aagcttcacggtgaccacgacggtcacggtaagcacaagggtcatggcgatcatgacggtcacggtaaacataagggccacggtgatcatgacggtcatggtaaacataagggttaataa BBa_K416003_sequence 1 atgaaagttttgattgttttgttggctattttcgctgctttgccattggctttggctcaaccagttatttctactactgttggttctgctgctgaaggttcactagataaaaga BBa_J63003_sequence 1 cccgccgccaccatggag BBa_K792013_sequence 1 ggatcc BBa_K792011_sequence 1 ggatcccccgccgccaccatggagatgaaagttttgattgttttgttggctattttcgctgctttgccattggctttggctcaaccagttatttctactactgttggttctgctgctgaaggttcactagataaaagaaagcttagaagaagaagaagaagaagacgtcgtcgtagaaagcttcacggtgaccacgacggtcacggtaagcacaagggtcatggcgatcatgacggtcacggtaaacataagggccacggtgatcatgacggtcatggtaaacataagggttaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z