BBa_K797004

BBa_K797004 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K797004

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Tomohiro Nobeyama

Date created: 2012-09-19 11:00:00

Date modified: 2015-05-08 01:13:23

Tat secretion cassette with constitutive promoter.

Details

• Types: DnaRegion
• Roles: Device; engineered_region
• Sequences: BBa_K797004_sequence (Version 1)

Description

This cassette allow E.coli to secrete proteins with torA signal. Wild type Tat protein secretion system is too week so that Kyoto 2012 construct Tat cassette to reinforce the ability of transportation of Tat system.
This parts include tatA,B,C protein coding region and pspA (phage shock protein A). Tat A,B,C protein is the main
component of Tat complex where proteins with torA signal go through and pspA can encourage protein secretion via Tat system. Kyoto 2012 suggest iGEMers with this new way of secretion and provide them with this cassette regulated by constitutive promoter.

Notes

This Device has too long sequence to read it so that we read each components' sequence separately and conjugated
them to make the information about sequence of the device

Source

tatABCD and pspA are derived from Escherichia coli's genomic sequence

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
J23107	1,35	promoter, feature/promoter
tatABCD	163,2554	feature/cds, CDS
pspA	2718,3386	CDS, feature/cds

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K797004/1

Member of these Collections

• iGEM 2019 Cell Tat secretion cassette with constitutive promoter.
• iGEM 2019 Distribution Plate 6
• iGEM 2019 Distribution Plate 6 Well 3F
• iGEM 2019 Part Library
• iGEM 2019 Plasmid pSB1C3
• iGEM Parts Registry