LOCUS       BBa_K797011             2139 bp    DNA     linear       13-May-2024
DEFINITION  Plasmid include T7 promoter, Histag and R9 peptide sequence
ACCESSION   BBa_K797011
VERSION     BBa_K797011.1  
FEATURES             Location/Qualifiers
     misc_feature    1..22
                     /label=prefix
     promoter        23..42
                     /label=T7
     RBS             54..65
                     /label=RBS
     misc_feature    75..92
                     /label=6His
     misc_feature    93..119
                     /label=R9
     misc_feature    121..141
                     /label=suffix
ORIGIN
        1 gaattcgcgg ccgcttctag agtaatacga ctcactatag ggagatacta gagaaagagg
       61 agaaatacta gatgcaccat caccatcacc atcgtcgtcg tcgtcgtcgt cgtcgtcgtt
      121 tactagtagc ggccgctgca gttgcgcgtg atcttacggc attatacgta tgatcggtcc
      181 acgatcagct agattatcta gtcagcttga tgtcatagct gtttcctgag gctcaatact
      241 gaccatttaa atcatacctg acctccatag cagaaagtca aaagcctccg accggaggct
      301 tttgacttga tcggcacgta agaggttcca actttcacca taatgaaata agatcactac
      361 cgggcgtatt ttttgagtta tcgagatttt caggagctaa ggaagctaaa atgagtattc
      421 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
      481 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
      541 acatcgaact ggatctcaac agcggtaaga tccttgagag tttacgcccc gaagaacgtt
      601 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
      661 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
      721 caccagtcac agaaaagcat ctcacggatg gcatgacagt aagagaatta tgcagtgctg
      781 ccataaccat gagtgataac actgcggcca acttacttct ggcaacgatc ggaggaccga
      841 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
      901 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
      961 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     1021 aattaataga ctggatggag gcggataaag ttgcaggatc acttctgcgc tcggccctcc
     1081 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     1141 ttgcagcact ggggccagat ggtaagccct cccgcatcgt agttatctac acgacgggga
     1201 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     1261 agcattggta atgagggccc aaatgtaatc acctggctca ccttcgggtg ggcctttctt
     1321 gaggacctaa atgtaatcac ctggctcacc ttcgggtggg cctttctgcg ttgctggcgt
     1381 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgatgctca agtcagaggt
     1441 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     1501 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     1561 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     1621 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     1681 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     1741 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     1801 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     1861 cctcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     1921 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     1981 gattttctac cgaagaaagg cccacccgtg taaaacgacg gccagtttat ctagtcagct
     2041 tgattctagc tgatcgtgga ccggaaggtg agccagtgag ttgattgcag tccagttacg
     2101 ctggagtctg aggctcgtcc tgaatgatat gcggcctcg
//