BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_I13453
1
BBa_I13453
Pbad promoter
2005-05-24T11:00:00Z
2015-08-31T04:07:34Z
Released HQ 2013
PBad promoter from I0500 without AraC.
false
false
_11_
0
253
6
In stock
false
true
jkm
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1686
1
T7 TE
range1686
1
8
27
annotation1690
1
polya
range1690
1
28
41
annotation1687
1
stop
range1687
1
34
34
BBa_K804007
1
BBa_K804007
Constitutive ECFP generator - Arabinose inducible TrpA coding gene
2012-10-01T11:00:00Z
2015-05-08T01:13:25Z
Strong Constitutive Promoter-ECFP generator- BBa_K804000
Pbad inducible Promoter- BBa_I13453
RBS-BBa_B0034
TrpA coding gene- BBa_K804002
Terminator- BBa_B0025
This part contains a constitutive ECFP generator along with an arabinose inducible (Pbad) Trp A coding gene. Upon induction with arabinose, TrpA is expressed for indole-3-glycerol-phosphate(InGP)binding and catalysis/cleavage of InGP to indole and glyceraldehyde-3-phosphate during an α reaction. This construct can be used to induce growth in Trp- auxotrophs and can be monitored by fluorescence for its population dynamics in co-culture with other auxotrophs.
false
false
_1060_
0
9774
9
It's complicated
false
None available.
false
Joe Ho
component2205679
1
BBa_I13453
component2205682
1
BBa_K804002
component2205678
1
BBa_K804000
component2205681
1
BBa_B0034
component2205685
1
BBa_B0025
annotation2205682
1
BBa_K804002
range2205682
1
1086
1892
annotation2205685
1
BBa_B0025
range2205685
1
1901
2029
annotation2205678
1
BBa_K804000
range2205678
1
1
921
annotation2205681
1
BBa_B0034
range2205681
1
1068
1079
annotation2205679
1
BBa_I13453
range2205679
1
930
1059
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation7018
1
BBa_B0010
range7018
1
1
80
annotation4184
1
stem_loop
range4184
1
12
55
BBa_E0020
1
ecfp
engineered cyan fluorescent protein derived from A. victoria GFP
2004-03-02T12:00:00Z
2015-08-31T04:07:25Z
Released HQ 2013
-- No description --
false
false
_1_
0
24
7
In stock
false
true
Caitlin Conboy and Jennifer Braff
BBa_B0025
1
BBa_B0025
double terminator (B0015), reversed
2003-12-02T12:00:00Z
2015-08-31T04:07:20Z
-- No description --
false
true
_1_
0
24
7
In stock
false
true
Caitlin Conboy
annotation369703
1
B0010
range369703
1
50
129
annotation369702
1
B0012
range369702
1
1
41
BBa_K804000
1
BBa_K804000
Strong Constitutive Promoter-ECFP generator
2012-09-30T11:00:00Z
2015-05-08T01:13:25Z
ECFP (BBa_E0420)
Constitutive Ptet promoter (BBa_J23118)
This is an Enhanced Cyan Fluorescence Protein under a strong constitutive Ptet promoter (BBa_J23118). It constitutively expresses ECFP (BBa_E0420). The CFP output device does not have a LVA tag and has a strong RBS. Under a plate scanner, ECFP excites at 439nm and emits at 476nm. The fluorescence output from this construct can be used to monitor growth and population dynamics(only at exponential phase) in a microbial consortium.
false
false
_1060_
0
9774
9
It's complicated
true
None available.
false
Joe Ho
component2205006
1
BBa_J23118
component2205016
1
BBa_E0420
annotation2205016
1
BBa_E0420
range2205016
1
44
921
annotation2205006
1
BBa_J23118
range2205006
1
1
35
BBa_K804002
1
BBa_K804002
TrpA coding gene
2012-09-30T11:00:00Z
2015-05-08T01:13:25Z
http://biocyc.org/ECOLI/sequence-rc?type=GENE&object=EG11024
This is a TrpA coding gene. It expresses a TrpA polypeptide(TSase α), which functions as both a binding site for indole-3-glycerol-phosphate (InGP) and catalysis/cleavage of InGP to indole and glyceraldehyde-3-phosphate during an α reaction.
false
false
_1060_
0
9774
9
In stock
false
None available.
false
Joe Ho
BBa_E0420
1
BBa_E0420
ECFP (RBS+ LVA- TERM) (B0034.E0020.B0015)
2004-03-15T12:00:00Z
2015-08-31T04:07:26Z
Released HQ 2013
Standard CFP output device w/o LVA tag.
false
true
_1_
0
24
7
In stock
false
true
Caitlin Conboy
component942797
1
BBa_B0012
component942787
1
BBa_B0010
component942780
1
BBa_B0034
component942782
1
BBa_E0020
annotation942797
1
BBa_B0012
range942797
1
838
878
annotation942780
1
BBa_B0034
range942780
1
1
12
annotation942782
1
BBa_E0020
range942782
1
19
741
annotation942787
1
BBa_B0010
range942787
1
750
829
BBa_J23118
1
BBa_J23118
constitutive promoter family member
2006-08-16T11:00:00Z
2015-08-31T04:08:40Z
Later
Released HQ 2013
Later
false
true
_52_
0
483
95
In stock
true
N/A
true
John Anderson
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_K804002_sequence
1
atggaacgctacgaatctctgtttgcccagttgaaggagcgcaaagaaggcgcattcgttcctttcgtcacgctcggtgatccgggcattgagcagtcattgaaaattatcgatacgctaattgaagccggtgctgacgcgctggagttaggtatccccttctccgacccactggcggatggcccgacgattcaaaacgccactctgcgcgcctttgcggcaggtgtgactccggcacaatgttttgaaatgctggcactgattcgccagaaacacccgaccattcccattggcctgttgatgtatgccaatctggtgtttaacaaaggcattgatgagttttatgcccagtgcgaaaaagtcggcgtcgattcggtgctggttgccgatgtgccagttgaagagtccgcgcccttccgccaggccgcgttgcgtcataatgtcgcacctatcttcatctgcccgccaaatgccgatgacgacctgctgcgccagatagcctcttacggtcgtggttacacctatttgctgtcacgagcaggcgtgaccggcgcagaaaaccgcgccgcgttacccctcaatcatctggttgcgaagctgaaagagtacaacgctgcacctccattgcagggatttggtatttccgccccggatcaggtaaaagcagcgattgatgcaggagctgcgggcgcgatttctggttcggccattgttaaaatcatcgagcaacatattaatgagccagagaaaatgctggcggcactgaaagtttttgtacaaccgatgaaagcggcgacgcgcagttaa
BBa_I13453_sequence
1
acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagc
BBa_E0020_sequence
1
atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataa
BBa_B0034_sequence
1
aaagaggagaaa
BBa_E0420_sequence
1
aaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K804000_sequence
1
ttgacggctagctcagtcctaggtattgtgctagctactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_J23118_sequence
1
ttgacggctagctcagtcctaggtattgtgctagc
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K804007_sequence
1
ttgacggctagctcagtcctaggtattgtgctagctactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggagaaatactagatggaacgctacgaatctctgtttgcccagttgaaggagcgcaaagaaggcgcattcgttcctttcgtcacgctcggtgatccgggcattgagcagtcattgaaaattatcgatacgctaattgaagccggtgctgacgcgctggagttaggtatccccttctccgacccactggcggatggcccgacgattcaaaacgccactctgcgcgcctttgcggcaggtgtgactccggcacaatgttttgaaatgctggcactgattcgccagaaacacccgaccattcccattggcctgttgatgtatgccaatctggtgtttaacaaaggcattgatgagttttatgcccagtgcgaaaaagtcggcgtcgattcggtgctggttgccgatgtgccagttgaagagtccgcgcccttccgccaggccgcgttgcgtcataatgtcgcacctatcttcatctgcccgccaaatgccgatgacgacctgctgcgccagatagcctcttacggtcgtggttacacctatttgctgtcacgagcaggcgtgaccggcgcagaaaaccgcgccgcgttacccctcaatcatctggttgcgaagctgaaagagtacaacgctgcacctccattgcagggatttggtatttccgccccggatcaggtaaaagcagcgattgatgcaggagctgcgggcgcgatttctggttcggccattgttaaaatcatcgagcaacatattaatgagccagagaaaatgctggcggcactgaaagtttttgtacaaccgatgaaagcggcgacgcgcagttaatactagagtataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctgg
BBa_B0025_sequence
1
tataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctgg
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z