BBa_K812012
1
BBa_K812012
OsTirI Ubiquitinase E3 for AID tagged protein degradation in the presence of auxin
2012-09-17T11:00:00Z
2015-05-08T01:13:27Z
This part has been extracted from the pNHK60 plasmid using PCR. THe pNHK60 plasmid was obtained from the riken registry.
OsTiRI is an ubuquitinase E3 that recognise and trigger the ubiquitination of every AID tagged protein. This part works with K812011 which is a AID tagged GFP. OsTirI is a protein comming from rice that have been identified as working in mammalian cells and yeast.
This part is a part of a device pattented by Kanemaki Masato, Kakimoto Tatsuo, Nishimura Kohei, Takisawa Haruhiko and Fukagawa Tatsuo for yeast and mammalian cells use (http://www.freepatentsonline.com/y2012/0115232.html). However the pattent does not cover the use for oviparian such as frogs and chicken.
This part contains 3 myc domain for protein staining using antiMyc antibodies
false
false
_1069_
0
8998
9
Not in stock
false
This part has been cloned in pSB1C3 using in-fusion and the illegal restriction site SpeI and PstI had been removed by PCR.
false
Cyrille Pauthenier
annotation2184379
1
Myc
range2184379
1
1793
1828
annotation2184378
1
Myc
range2184378
1
1757
1792
annotation2184380
1
Myc
range2184380
1
1829
1864
annotation2184376
1
kozac
range2184376
1
1
7
annotation2184382
1
SpeI elimitated
range2184382
1
1156
1156
annotation2184377
1
OsTirI
range2184377
1
8
1732
annotation2184381
1
PstI eliminated
range2184381
1
1066
1066
BBa_K812012_sequence
1
agccaccatgacgtacttcccggaggaggtggtggagcacatcttcagcttcctgccggcgcagcgcgaccgcaacacggtctcgctcgtctgcaaggtgtggtacgagatcgagaggctgagccgccgcggcgtcttcgtgggcaactgctacgccgtgcgcgccggccgcgtcgccgcgcggttccccaacgtgcgggcgctcacggtgaaggggaagccccacttcgccgacttcaacctcgtgccccccgactggggcggctacgcggggccgtggatcgaggcggccgcgaggggatgccacggcctggaggagctcaggatgaagcggatggtggtgtccgacgagagcctcgagctgctggctcgctcgttcccgcggttcagggctcttgttcttatcagctgcgaggggttcagcactgacgggctagccgccgtcgcgagccattgcaagcttctgagggagttggatttgcaggaaaatgaagtggaggatcgagggcctaggtggctttcctgcttccctgattcctgcacatcacttgtctcattgaattttgcctgcatcaaaggggaggttaatgctggttcactggagagacttgttagcaggtccccaaacctgcggagtttgaggctgaatcgatctgtatcggtagatacacttgcaaagatactactgcgtacccctaacttggaggatttggggacagggaatttgacagatgacttccaaactgagtcctactttaagcttaccagtgctctggagaaatgcaagatgttgaggagtttgtctggattctgggatgcttctcctgtttgcctgtcatttatctaccccctgtgtgctcaactgacaggattgaacttgagctatgcccccacacttgatgcttctgaccttacaaaaatgattagccgctgtgtgaagctccaacgcctttgggtactggattgtatctcggacaaaggcttgcaagtggtggcctccagttgcaaagacttgcaagaactcagggtatttccatcagatttctacgtagctggttattctgctgtgacagaggagggacttgttgcagtatccttgggctgtccaaaactgaactcactactgtacttctgtcaccaaatgactaatgctgctctagttactgtcgccaagaactgtccaaatttcacacgattcagactttgtattcttgagccagggaagcctgatgttgtgacaagccaaccattagatgaaggctttggagctattgttcgtgagtgcaagggattacaacgtttgtcaatatctggtcttctcacagacaaagttttcatgtatattgggaaatatgcaaaacaacttgagatgctttctatagcatttgctggtgacagtgataagggtatgatgcatgttatgaatggatgcaagaatttaaggaaactggagataagagatagcccgtttggtgatgctgcactcttggggaattttgctaggtacgagacaatgcgatccctttggatgtcatcttgcaatgtcacgttaaaggggtgccaagtccttgcgtcaaagatgccgatgctcaatgttgaggtcataaatgagcgggatggtagcaatgaaatggaggaaaaccatggagatctgcctaaagtggagaaattatatgtgtaccgcacaactgctggggcgagggatgatgcaccaaattttgttaaaatcctagtcgactccggttctgctgctagtggtgaacaaaagttgatttctgaagaagatttgaacggggaacaaaagctaatctccgaggaagacttgaacggtgaacaaaaattaatctcagaagaagacttgaactaataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z