BBa_K812014 1 BBa_K812014 Auxin production device for expression in tadpole 2012-09-17T11:00:00Z 2015-05-08T01:13:27Z The different protein has been amplified from the bacterial auxin production device of iGEM Imperial 2011 K515100 This device is ment for the co-expression of IaaH and IaaM gene in the same cell in tadpole. The creation of polysistronic mRNA in tadpole doesn't work very well. This is why the two proteins are fused with a self cleavable peptide domain Pep2A which allow the protein to be transcribed as a single peptide that is after cleaved in two different proteins that fold later on. This part is to create auxin in tadpole as the emmitor device of our sythetic hormonal system. Have a look at our wiki to undersand how this system is supposed to work: http://2012.igem.org/Team:Evry/FrenchFrog. This part can be used in an oviparian plasmid such as pSC2+ (see K812000) false false _1069_ 0 8998 9 It's complicated false This part has been assembled using golden gate technique. The biobrick site are a bit different than the usual one but can still be assembled using the RFC 10 system. false Cyrille Pauthenier annotation2184391 1 IaaM CDS range2184391 1 1415 3052 annotation2184389 1 IaaH CDS range2184389 1 8 1351 annotation2184390 1 Pep2A self cleavable peptide range2184390 1 1352 1414 annotation2184388 1 kozac range2184388 1 1 7 BBa_K812014_sequence 1 agccaccatgcgcgaaatgattacgcttgaatctctgtgccgcgcattggcagacgaacagattgccgcagaagaattgcgcgagcgcgcgctggatacagaggctcgcttaacactgttaaattgcttcattcgcgaaggcgacgccgtaagccaattcggtgaagcggatcaggcgcgtaaaggaacttcactttggggggtgcctgtgagttttaaggataacatctgtgttcgcgggctgccactgacggcgggtacccgcggcatgagcggctttattgccgatcaggatgcagcaatcgtgagccagttaaaagcattgggagctgtggtcgcgggcaaaaataacatgcatgagctgtcatttggcgtcacgagcatcaatccacattggggcgcggttggaaacccggtggctccaggatattgcgccggaggttctagcggaggttccgctgcggcggtcgcatctggaattgtcccgctcagcgtcgggacggacacaggcggctcaatccgtattccggcggcattttgcggcattacgggttttcgccctactacaggtcgcttgagtacggcggggatcattccggtcagccacacgaaggattgtgtcggcctcctgacgcgcacagctggcgacgctgaatttgtgtatgggctgctgtcaggaaaacagcagagtttccctcttaatcgcacgggaccttgccgcatcggcctgccggtgagcatgtggtctgacttagatggcgaagtagaacgtgcctgtatcaatgcgctgagcctcttacgcaaaacgggctttgaatttgttgagatcgacgatgcggacattgtggaattgaaccagacattaaccttcacggtgccgctgtacgaatttttcgcggattttgctcaatccttgttgtccttaggttggaaacatggcatccatcatatttttgcacaagtggatgatgcaaatgtgaaaggcattattaatcaccatctgggagagggcgcgattaagccggcacattatctgagttctctccagaatggagagctgctgaaacgcaagatggatgagctttttgcgcgccatcacattaaacttttagggtatccgacggtcccgtgtcgcgtgcctcatctggaccatgcagatcgtccggaattttttagccaggccatccgtaacacagacctcgcaagcaacgcgatgttaccaagcattacaattccggtcggtccggaaggccgcctgccggtcggccttagttttgatgcgcctcgtgctcgtgatgcgtttctcttaagcaatgttagtttgatcgaaaaagtgttaaaaggctaataaggcagtggagagggcagaggaagtctgctaacatgcggtgacgtcgaggagaatcctggcccaatgtttggaccggatttcccgtttgcatttgatgattggatcgaacatcctaaaggacttgggtcaattccggcagaaaatcacggcgctgaggttgcaattatcggggccggcattgcaggcttagtagcagcatatgaattaatgaagatgggtctgaaacctgtagtctatgaggcctccaaaatgggaggtcgcttacggtcacaggaatttgaaggagcgaagggaattgttgcggaactgggcggtatgcgctttccagtctcctccacagcgttctttcattacgttgataaactgggccttgaaagtcgtccgttccctaaccctttgacggcggccagtggcagcacggtgattgacctggaaggtaccacgtactatgcacagatgttatcagatctcccagccctgttccaagaagtggcggatgcatgggcagacgccttagaatcaggctctcaatttggagatattcaacaggccattcgtgattctgacgtaccgcgtctcaaggaactgtggaacaaactcgtgcctttgtgggacgatcgcactttctatgactttgtagcgacctcaaaggcctttgcgaaattaagcttttaccatcgtgaggttttcggacaagttggttttggtacaggggggtgggattctgattttccaaatagtatgttagaaatctttcgtgttgtgatgacgaattgtgatgagcaccagcatctgattgttggtggcgtacagcaagtgcctgtaggcctgtggtcgcacgtgcctgaacgctgcgcacattggccaaagggtacgtctctttcctcacttcatcggggtgccccgcgcccaggtgtcaaacgtattgcacgtgctgaagatggttcatttacggttacagataattggggcgatacacgtcaatacgctgccgttttgacaacctgtcaatcgtggctgcttacaacgcagattgaatgtgaggaatctttgttttcacagaagatgtggatggcgctggatcgcacgcgttacatgcagtcttctaaaacttttgtgatggttgatcggccgttttggaaggacaaagatccggaaacgggtcgcgatcttatgtccatgaccctcaccgatcgcttaacgcgcggcacatacttatttgataatggcgacgacaagccaggcgttatttgcctgagttactcatggatgagtgacgctttaaaaatgttaccgcaaccgatcgagaaacgcgtcaaattagctttagatgcccttaaaaagatttacccgaaagtggatatcgccgcacgtattattggtgacccgattactgtgagttgggaagcggatccgcatttcctgggggcatttaaaggcgcgttaccgggccattaccgttacaaccagcgcatgtacgcgcattttatgcagcaggacatgccgtcagaacagcgtggcatgtttattgccggtgatgatgtatcttggacaccggcatgggttgagggagcagtacaaacgtcattgaacgcggtgtggggcatcatgaaccatttcggagggaaaacccacgcagagaatccgggtcctggcgacgttttccatgagattggaccgatcgcgctgggggactaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z