LOCUS       BBa_K812050             2084 bp    DNA     linear       17-May-2024
DEFINITION  Golden brick version of pSB1C3 plasmid for Golden Gate and Golden 
            Brick cloning
ACCESSION   BBa_K812050
VERSION     BBa_K812050.1  
FEATURES             Location/Qualifiers
     misc_feature    1..27
                     /label=Golden Brick Back
     misc_feature    1..6
                     /label=SpeI
     misc_feature    7..12
                     /label=BsaI
     misc_feature    23..27
                     /label=PstI
     misc_feature    17..22
                     /label=BbsI
     misc_feature    14..17
                     /label=OH5
     misc_feature    28..2057
                     /label=pSB1C3 backbone
     stem_loop       29..101
                     /label=E. coli His Operon terminator
     primer_bind     165..184
                     /label=VR
     misc_feature    269..883
                     /label=pMB1 replication origin
     stem_loop       1050..1155
                     /label=T0 terminator
     CDS             1168..1827
                     /label=Cloramphenicol resistance CDS
     primer_bind     1941..1960
                     /label=VF2
     misc_feature    2030..2049
                     /label=Terminator
     misc_feature    2058..2084
                     /label=Golden Brick Back
     misc_feature    2058..2062
                     /label=EcoRI
     misc_feature    2063..2068
                     /label=Bbsi
     misc_feature    2068..2071
                     /label=OH1
     misc_feature    2073..2078
                     /label=BsaI
     misc_feature    2079..2084
                     /label=XbaI
ORIGIN
        1 actagtggtc tccagcgtct tcctgcagtc cggcaaaaaa acggcaaggt gtcaccaccc
       61 tgcccttttt ctttaaaacc gaaaagatta cttcgcgtta tgcaggcttc ctcgctcact
      121 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
      181 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
      241 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccacag gctccgcccc
      301 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
      361 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
      421 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
      481 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
      541 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
      601 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
      661 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
      721 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
      781 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
      841 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
      901 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
      961 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     1021 gagtaaactt ggtctgacag ctcgaggctt ggattctcac caataaaaaa cgcccggcgg
     1081 caaccgagcg ttctgaacaa atccagatgg agttctgagg tcattactgg atctatcaac
     1141 aggagtccaa gcgagctcga tatcaaatta cgccccgccc tgccactcat cgcagtactg
     1201 ttgtaattca ttaagcattc tgccgacatg gaagccatca caaacggcat gatgaacctg
     1261 aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac
     1321 gggggcgaag aagttgtcca tattggccac gtttaaatca aaactggtga aactcaccca
     1381 gggattggct gagacgaaaa acatattctc aataaaccct ttagggaaat aggccaggtt
     1441 ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg
     1501 gtattcactc cagagcgatg aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg
     1561 gtgaacacta tcccatatca ccagctcacc gtctttcatt gccatacgaa attccggatg
     1621 agcattcatc aggcgggcaa gaatgtgaat aaaggccgga taaaacttgt gcttattttt
     1681 ctttacggtc tttaaaaagg ccgtaatatc cagctgaacg gtctggttat aggtacattg
     1741 agcaactgac tgaaatgcct caaaatgttc tttacgatgc cattgggata tatcaacggt
     1801 ggtatatcca gtgatttttt tctccatttt agcttcctta gctcctgaaa atctcgataa
     1861 ctcaaaaaat acgcccggta gtgatcttat ttcattatgg tgaaagttgg aacctcttac
     1921 gtgcccgatc aactcgagtg ccacctgacg tctaagaaac cattattatc atgacattaa
     1981 cctataaaaa taggcgtatc acgaggcaga atttcagata aaaaaaatcc ttagctttcg
     2041 ctaaggatga tttctggaat tcgaagacgc ttgagacctc taga
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