BBa_K812050
1
pSB1C3 GB
Golden brick version of pSB1C3 plasmid for Golden Gate and Golden Brick cloning
2012-09-19T11:00:00Z
2015-05-08T01:13:27Z
This part is a derivation of the pSB1C3 plasmid from the registry.
This is a modified version of pSB1C3 from the registry with the J4450 negative control. The biobrick sites are modified to incorporate a BsaI restriction site that enable to the use of golden gate cloning and golden brick cloning to assemble full contructs in a single shot of digestion/ligation.
The complete protocol of the goldengate can be find in the original paper: Engler C, Gruetzner R, Kandzia R, Marillonnet S (2009) Golden Gate Shuffling: A One-Pot DNA Shuffling Method Based on Type IIs Restriction
Enzymes. PLoS ONE 4(5): e5553. doi:10.1371/journal.pone.0005553
The Golden Brick assembly is an invention of the iGEM Evry 2012 Team. A complete description of the procedure can be find on our website: http://2012.igem.org/Team:Evry/GB
false
false
_1069_
0
8998
9
Not in stock
true
The negative control J4450 has been amplified with new goldenbrick extensions using PCR and re-inserted in pSB1C3 using EcoRI and PstI cloning.
false
Cyrille Pauthenier
annotation2187962
1
OH1
range2187962
1
2068
2071
annotation2187767
1
Cloramphenicol resistance CDS
range2187767
1
1168
1827
annotation2187752
1
SpeI
range2187752
1
1
6
annotation2187897
1
EcoRI
range2187897
1
2058
2062
annotation2187766
1
T0 terminator
range2187766
1
1050
1155
annotation2187768
1
VF2
range2187768
1
1941
1960
annotation2187792
1
Golden Brick Back
range2187792
1
2058
2084
annotation2188002
1
XbaI
range2188002
1
2079
2084
annotation2187963
1
BsaI
range2187963
1
2073
2078
annotation2187757
1
OH5
range2187757
1
14
17
annotation2187930
1
Bbsi
range2187930
1
2063
2068
annotation2187774
1
Terminator
range2187774
1
2030
2049
annotation2187755
1
BbsI
range2187755
1
17
22
annotation2187753
1
BsaI
range2187753
1
7
12
annotation2187749
1
Golden Brick Back
range2187749
1
1
27
annotation2187754
1
PstI
range2187754
1
23
27
annotation2187762
1
E. coli His Operon terminator
range2187762
1
29
101
annotation2187764
1
pMB1 replication origin
range2187764
1
269
883
annotation2187758
1
pSB1C3 backbone
range2187758
1
28
2057
annotation2187763
1
VR
range2187763
1
165
184
BBa_K812050_sequence
1
actagtggtctccagcgtcttcctgcagtccggcaaaaaaacggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgttatgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccacaggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaagaacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagctcgaggcttggattctcaccaataaaaaacgcccggcggcaaccgagcgttctgaacaaatccagatggagttctgaggtcattactggatctatcaacaggagtccaagcgagctcgatatcaaattacgccccgccctgccactcatcgcagtactgttgtaattcattaagcattctgccgacatggaagccatcacaaacggcatgatgaacctgaatcgccagcggcatcagcaccttgtcgccttgcgtataatatttgcccatggtgaaaacgggggcgaagaagttgtccatattggccacgtttaaatcaaaactggtgaaactcacccagggattggctgagacgaaaaacatattctcaataaaccctttagggaaataggccaggttttcaccgtaacacgccacatcttgcgaatatatgtgtagaaactgccggaaatcgtcgtggtattcactccagagcgatgaaaacgtttcagtttgctcatggaaaacggtgtaacaagggtgaacactatcccatatcaccagctcaccgtctttcattgccatacgaaattccggatgagcattcatcaggcgggcaagaatgtgaataaaggccggataaaacttgtgcttatttttctttacggtctttaaaaaggccgtaatatccagctgaacggtctggttataggtacattgagcaactgactgaaatgcctcaaaatgttctttacgatgccattgggatatatcaacggtggtatatccagtgatttttttctccattttagcttccttagctcctgaaaatctcgataactcaaaaaatacgcccggtagtgatcttatttcattatggtgaaagttggaacctcttacgtgcccgatcaactcgagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctggaattcgaagacgcttgagacctctaga
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z