BBa_K812050 1 pSB1C3 GB Golden brick version of pSB1C3 plasmid for Golden Gate and Golden Brick cloning 2012-09-19T11:00:00Z 2015-05-08T01:13:27Z This part is a derivation of the pSB1C3 plasmid from the registry. This is a modified version of pSB1C3 from the registry with the J4450 negative control. The biobrick sites are modified to incorporate a BsaI restriction site that enable to the use of golden gate cloning and golden brick cloning to assemble full contructs in a single shot of digestion/ligation. The complete protocol of the goldengate can be find in the original paper: Engler C, Gruetzner R, Kandzia R, Marillonnet S (2009) Golden Gate Shuffling: A One-Pot DNA Shuffling Method Based on Type IIs Restriction Enzymes. PLoS ONE 4(5): e5553. doi:10.1371/journal.pone.0005553 The Golden Brick assembly is an invention of the iGEM Evry 2012 Team. A complete description of the procedure can be find on our website: http://2012.igem.org/Team:Evry/GB false false _1069_ 0 8998 9 Not in stock true The negative control J4450 has been amplified with new goldenbrick extensions using PCR and re-inserted in pSB1C3 using EcoRI and PstI cloning. false Cyrille Pauthenier annotation2187962 1 OH1 range2187962 1 2068 2071 annotation2187767 1 Cloramphenicol resistance CDS range2187767 1 1168 1827 annotation2187752 1 SpeI range2187752 1 1 6 annotation2187897 1 EcoRI range2187897 1 2058 2062 annotation2187766 1 T0 terminator range2187766 1 1050 1155 annotation2187768 1 VF2 range2187768 1 1941 1960 annotation2187792 1 Golden Brick Back range2187792 1 2058 2084 annotation2188002 1 XbaI range2188002 1 2079 2084 annotation2187963 1 BsaI range2187963 1 2073 2078 annotation2187757 1 OH5 range2187757 1 14 17 annotation2187930 1 Bbsi range2187930 1 2063 2068 annotation2187774 1 Terminator range2187774 1 2030 2049 annotation2187755 1 BbsI range2187755 1 17 22 annotation2187753 1 BsaI range2187753 1 7 12 annotation2187749 1 Golden Brick Back range2187749 1 1 27 annotation2187754 1 PstI range2187754 1 23 27 annotation2187762 1 E. coli His Operon terminator range2187762 1 29 101 annotation2187764 1 pMB1 replication origin range2187764 1 269 883 annotation2187758 1 pSB1C3 backbone range2187758 1 28 2057 annotation2187763 1 VR range2187763 1 165 184 BBa_K812050_sequence 1 actagtggtctccagcgtcttcctgcagtccggcaaaaaaacggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgttatgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccacaggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaagaacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagctcgaggcttggattctcaccaataaaaaacgcccggcggcaaccgagcgttctgaacaaatccagatggagttctgaggtcattactggatctatcaacaggagtccaagcgagctcgatatcaaattacgccccgccctgccactcatcgcagtactgttgtaattcattaagcattctgccgacatggaagccatcacaaacggcatgatgaacctgaatcgccagcggcatcagcaccttgtcgccttgcgtataatatttgcccatggtgaaaacgggggcgaagaagttgtccatattggccacgtttaaatcaaaactggtgaaactcacccagggattggctgagacgaaaaacatattctcaataaaccctttagggaaataggccaggttttcaccgtaacacgccacatcttgcgaatatatgtgtagaaactgccggaaatcgtcgtggtattcactccagagcgatgaaaacgtttcagtttgctcatggaaaacggtgtaacaagggtgaacactatcccatatcaccagctcaccgtctttcattgccatacgaaattccggatgagcattcatcaggcgggcaagaatgtgaataaaggccggataaaacttgtgcttatttttctttacggtctttaaaaaggccgtaatatccagctgaacggtctggttataggtacattgagcaactgactgaaatgcctcaaaatgttctttacgatgccattgggatatatcaacggtggtatatccagtgatttttttctccattttagcttccttagctcctgaaaatctcgataactcaaaaaatacgcccggtagtgatcttatttcattatggtgaaagttggaacctcttacgtgcccgatcaactcgagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctggaattcgaagacgcttgagacctctaga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z