BBa_K812053
1
BBa_K812053
RBS B0034 with GoldenBrick sequence
2012-09-19T11:00:00Z
2015-05-08T01:13:27Z
This part has been amplified from B0034 with GoldenBrick extension
This part is the GoldenBricked version of B0034 RBS. The sequence is the same but the biobrick sequence have been modified to enable GoldenBrick assembly, adding a BsaI site and the possibility of BbsI digestion of the scar once formed.
To learn more about the GoldenBrick assembly our team have invented this year, go to: http://2012.igem.org/Team:Evry/GB
false
false
_1069_
0
8998
9
It's complicated
false
This part has been amplified from B0034 with GoldenBrick extension by PCR and cloned in pSB1C3 using EcoRI and PstI
false
Cyrille Pauthenier
annotation2201457
1
XbaI
range2201457
1
11
16
annotation2201450
1
GoldenBricked RBS B0034
range2201450
1
18
29
annotation2201453
1
EcoRI
range2201453
1
1
6
annotation2201454
1
BsalI
range2201454
1
7
12
annotation2201460
1
BsaI
range2201460
1
37
42
annotation2201464
1
Spacer
range2201464
1
30
30
annotation2201465
1
OH3
range2201465
1
32
35
annotation2201449
1
GoldenBrick RBS Prefix
range2201449
1
1
17
annotation2201462
1
PstI
range2201462
1
43
47
annotation2201459
1
SpeI
range2201459
1
31
36
annotation2201463
1
OH2
range2201463
1
14
17
annotation2201451
1
Goldenbrick RBS Sufix
range2201451
1
30
47
BBa_K812053_sequence
1
gaattcggtctctagacaaagaggagaaatactagtgagaccctgca
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z