BBa_K812055
1
BBa_K812055
Terminator B0015 with GoldenBrick extension
2012-09-19T11:00:00Z
2015-05-08T01:13:27Z
The part B0015 has been amplified by PCR with the new GoldenBrick extension, and then cloned in pSB1C3 using EcoRI and PstI.
B0015 terminator with GoldenBrick extension
This part is the GoldenBricked version of R0010 pLac promoter. The sequence is the same but the biobrick sequence have been modified to enable GoldenBrick assembly, adding a BsaI site and the possibility of BbsI digestion of the scar once formed.
To learn more about the GoldenBrick assembly our team have invented this year, go to: http://2012.igem.org/Team:Evry/GB
false
false
_1069_
0
8998
9
It's complicated
false
This part has been created from B0015 by PCR amplification
false
Cyrille Pauthenier
annotation2201984
1
EcoRI
range2201984
1
1
6
annotation2201839
1
GoldenBrick Terminator prefix
range2201839
1
1
17
annotation2202112
1
BsaI
range2202112
1
159
164
annotation2202099
1
BsaI
range2202099
1
7
12
annotation2202110
1
SpeI
range2202110
1
148
153
annotation2202102
1
XbaI
range2202102
1
11
16
annotation2202106
1
GoldenBrick Terminator suffix
range2202106
1
147
169
annotation2202113
1
PstI
range2202113
1
165
169
annotation2202111
1
OH5
range2202111
1
154
157
annotation2202105
1
BBa_B0012
range2202105
1
106
146
annotation2202103
1
BBa_B0010
range2202103
1
18
97
annotation2202101
1
OH4
range2202101
1
14
17
BBa_K812055_sequence
1
gaattcggtctctagacccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagtagcgggagaccctgca
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z