LOCUS BBa_K817032 3427 bp DNA linear 23-May-2024 DEFINITION PfadBA-TetR(P1040)-pLac ACCESSION BBa_K817032 VERSION BBa_K817032.1 FEATURES Location/Qualifiers promoter 1..72 /label=PfadBA promoter 1..72 /label=BBa_K817002 RBS 81..91 /label=RBS-4\Weaker RBS 87..90 /label=RBS misc_feature 81..91 /label=BBa_B0033 RBS 81..91 /label=BBa_B0033 CDS 101..717 /label=tetR misc_feature 718..751 /label=SsrA misc_feature 758..782 /label=Help:Barcodes CDS 98..757 /label=BBa_C0040 stem_loop 802..845 /label=stem_loop misc_feature 791..870 /label=BBa_B0010 terminator 791..870 /label=BBa_B0010 stem_loop 886..905 /label=T7 TE misc_feature 912..912 /label=stop polyA_site 906..919 /label=polya misc_feature 879..919 /label=BBa_B0012 terminator 879..919 /label=BBa_B0012 misc_feature 81..919 /label=BBa_P0340 misc_feature 928..946 /label=TetR 1 misc_feature 928..981 /label=BBa_R0040 promoter 947..952 /label=-35 misc_feature 953..971 /label=TetR 2 promoter 970..975 /label=-10 promoter 928..981 /label=BBa_R0040 misc_feature 994..997 /label=conserved RBS 990..1001 /label=BBa_B0034 misc_feature 2097..2135 /label=LVA CDS 1008..2135 /label=lacI-LVA misc_feature 1008..2135 /label=BBa_C0012 misc_feature 2136..2160 /label=Help:Barcodes CDS 1008..2135 /label=BBa_C0012 misc_feature 990..2160 /label=BBa_S0100 misc_feature 928..2160 /label=BBa_I763025 stem_loop 2180..2223 /label=stem_loop misc_feature 2169..2248 /label=BBa_B0010 terminator 2169..2248 /label=BBa_B0010 stem_loop 2264..2283 /label=T7 TE misc_feature 2290..2290 /label=stop polyA_site 2284..2297 /label=polya misc_feature 2257..2297 /label=BBa_B0012 terminator 2257..2297 /label=BBa_B0012 terminator 2169..2297 /label=BBa_B0015 misc_feature 928..2297 /label=BBa_I763026 CDS 2306..2393 /label=end of LacI coding region (inactive) misc_feature 2306..2505 /label=BBa_R0010 misc_feature 2394..2431 /label=CAP binding site promoter 2442..2447 /label=-35 promoter 2466..2471 /label=-10 misc_feature 2471..2505 /label=LacI binding site misc_feature 2478..2478 /label=start promoter 2306..2505 /label=BBa_R0010 misc_feature 2518..2521 /label=conserved RBS 2514..2525 /label=BBa_B0034 misc_feature 2532..3251 /label=BBa_E0040 misc_feature 3246..3284 /label=LVA misc_feature 3285..3290 /label=Double Stop misc_feature 2532..2534 /label=Start misc_feature 2532..3290 /label=BBa_J04031 stem_loop 3310..3353 /label=stem_loop misc_feature 3299..3378 /label=BBa_B0010 terminator 3299..3378 /label=BBa_B0010 stem_loop 3394..3413 /label=T7 TE misc_feature 3420..3420 /label=stop polyA_site 3414..3427 /label=polya misc_feature 3387..3427 /label=BBa_B0012 terminator 3387..3427 /label=BBa_B0012 misc_feature 2306..3427 /label=BBa_I763004 misc_feature 928..3427 /label=BBa_I763027 ORIGIN 1 atctggtacg accagatttg acaatctggt acgaccagat gatactgagc acatcagcag 61 gacgcactga cctactagag tcacacagga ctactagatg tccagattag ataaaagtaa 121 agtgattaac agcgcattag agctgcttaa tgaggtcgga atcgaaggtt taacaacccg 181 taaactcgcc cagaagctag gtgtagagca gcctacattg tattggcatg taaaaaataa 241 gcgggctttg ctcgacgcct tagccattga gatgttagat aggcaccata ctcacttttg 301 ccctttagaa ggggaaagct ggcaagattt tttacgtaat aacgctaaaa gttttagatg 361 tgctttacta agtcatcgcg atggagcaaa agtacattta ggtacacggc ctacagaaaa 421 acagtatgaa actctcgaaa atcaattagc ctttttatgc caacaaggtt tttcactaga 481 gaatgcatta tatgcactca gcgctgtggg gcattttact ttaggttgcg tattggaaga 541 tcaagagcat caagtcgcta aagaagaaag ggaaacacct actactgata gtatgccgcc 601 attattacga caagctatcg aattatttga tcaccaaggt gcagagccag ccttcttatt 661 cggccttgaa ttgatcatat gcggattaga aaaacaactt aaatgtgaaa gtgggtccgc 721 tgcaaacgac gaaaactacg ctttagtagc ttaataacac tgatagtgct agtgtagatc 781 actactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt 841 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt 901 gggcctttct gcgtttatat actagagtcc ctatcagtga tagagattga catccctatc 961 agtgatagag atactgagca ctactagaga aagaggagaa atactagatg gtgaatgtga 1021 aaccagtaac gttatacgat gtcgcagagt atgccggtgt ctcttatcag accgtttccc 1081 gcgtggtgaa ccaggccagc cacgtttctg cgaaaacgcg ggaaaaagtg gaagcggcga 1141 tggcggagct gaattacatt cccaaccgcg tggcacaaca actggcgggc aaacagtcgt 1201 tgctgattgg cgttgccacc tccagtctgg ccctgcacgc gccgtcgcaa attgtcgcgg 1261 cgattaaatc tcgcgccgat caactgggtg ccagcgtggt ggtgtcgatg gtagaacgaa 1321 gcggcgtcga agcctgtaaa gcggcggtgc acaatcttct cgcgcaacgc gtcagtgggc 1381 tgatcattaa ctatccgctg gatgaccagg atgccattgc tgtggaagct gcctgcacta 1441 atgttccggc gttatttctt gatgtctctg accagacacc catcaacagt attattttct 1501 cccatgaaga cggtacgcga ctgggcgtgg agcatctggt cgcattgggt caccagcaaa 1561 tcgcgctgtt agcgggccca ttaagttctg tctcggcgcg tctgcgtctg gctggctggc 1621 ataaatatct cactcgcaat caaattcagc cgatagcgga acgggaaggc gactggagtg 1681 ccatgtccgg ttttcaacaa accatgcaaa tgctgaatga gggcatcgtt cccactgcga 1741 tgctggttgc caacgatcag atggcgctgg gcgcaatgcg cgccattacc gagtccgggc 1801 tgcgcgttgg tgcggatatc tcggtagtgg gatacgacga taccgaagac agctcatgtt 1861 atatcccgcc gttaaccacc atcaaacagg attttcgcct gctggggcaa accagcgtgg 1921 accgcttgct gcaactctct cagggccagg cggtgaaggg caatcagctg ttgcccgtct 1981 cactggtgaa aagaaaaacc accctggcgc ccaatacgca aaccgcctct ccccgcgcgt 2041 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcaggctg 2101 caaacgacga aaactacgct ttagtagctt aataactctg atagtgctag tgtagatctc 2161 tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt 2221 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg 2281 gcctttctgc gtttatatac tagagcaata cgcaaaccgc ctctccccgc gcgttggccg 2341 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac 2401 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg 2461 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacatacta gagaaagagg 2521 agaaatacta gatgcgtaaa ggagaagaac ttttcactgg agttgtccca attcttgttg 2581 aattagatgg tgatgttaat gggcacaaat tttctgtcag tggagagggt gaaggtgatg 2641 caacatacgg aaaacttacc cttaaattta tttgcactac tggaaaacta cctgttccat 2701 ggccaacact tgtcactact ttcggttatg gtgttcaatg ctttgcgaga tacccagatc 2761 atatgaaaca gcatgacttt ttcaagagtg ccatgcccga aggttatgta caggaaagaa 2821 ctatattttt caaagatgac gggaactaca agacacgtgc tgaagtcaag tttgaaggtg 2881 atacccttgt taatagaatc gagttaaaag gtattgattt taaagaagat ggaaacattc 2941 ttggacacaa attggaatac aactataact cacacaatgt atacatcatg gcagacaaac 3001 aaaagaatgg aatcaaagtt aacttcaaaa ttagacacaa cattgaagat ggaagcgttc 3061 aactagcaga ccattatcaa caaaatactc caattggcga tggccctgtc cttttaccag 3121 acaaccatta cctgtccaca caatctgccc tttcgaaaga tcccaacgaa aagagagacc 3181 acatggtcct tcttgagttt gtaacagctg ctgggattac acatggcatg gatgaactat 3241 acaaaaggcc tgctgcaaac gacgaaaact acgctttagt agcttaataa tactagagcc 3301 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt 3361 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc 3421 gtttata //