LOCUS       BBa_K824008             1068 bp    DNA     linear       18-May-2024
DEFINITION  Cadmium Promoter with GFP Reporter
ACCESSION   BBa_K824008
VERSION     BBa_K824008.1  
FEATURES             Location/Qualifiers
     misc_feature    9..26
                     /label=ArsR binding site
     promoter        1..26
                     /label=BBa_K174016
     promoter        45..178
                     /label=cadA promoter
     misc_feature    52..57
                     /label=sigA -35
     misc_feature    76..81
                     /label=sigA -10
     misc_feature    90..120
                     /label=CzrA binding site
     promoter        35..205
                     /label=BBa_K174017
     promoter        1..205
                     /label=BBa_K174015
     CDS             212..931
                     /label=GFP protein
     CDS             212..931
                     /label=BBa_E0040
     stem_loop       951..994
                     /label=stem_loop
     misc_feature    940..1019
                     /label=BBa_B0010
     terminator      940..1019
                     /label=BBa_B0010
     stem_loop       1035..1054
                     /label=T7 TE
     misc_feature    1061..1061
                     /label=stop
     polyA_site      1055..1068
                     /label=polya
     misc_feature    1028..1068
                     /label=BBa_B0012
     terminator      1028..1068
                     /label=BBa_B0012
     misc_feature    212..1068
                     /label=BBa_I13401
ORIGIN
        1 agtaatcaaa ataaattgat ttattttact agaggcgctt gctgtttttc attgacactt
       61 tcttggaaaa caacatataa taggtgtaac ttatatatga gtatatgctc atatatataa
      121 aataaataca atactcattg atacgctttg aagagggaac cctaaatcgg aaggtaagct
      181 aagaggagga actactatgg ctagctacta gatgcgtaaa ggagaagaac ttttcactgg
      241 agttgtccca attcttgttg aattagatgg tgatgttaat gggcacaaat tttctgtcag
      301 tggagagggt gaaggtgatg caacatacgg aaaacttacc cttaaattta tttgcactac
      361 tggaaaacta cctgttccat ggccaacact tgtcactact ttcggttatg gtgttcaatg
      421 ctttgcgaga tacccagatc atatgaaaca gcatgacttt ttcaagagtg ccatgcccga
      481 aggttatgta caggaaagaa ctatattttt caaagatgac gggaactaca agacacgtgc
      541 tgaagtcaag tttgaaggtg atacccttgt taatagaatc gagttaaaag gtattgattt
      601 taaagaagat ggaaacattc ttggacacaa attggaatac aactataact cacacaatgt
      661 atacatcatg gcagacaaac aaaagaatgg aatcaaagtt aacttcaaaa ttagacacaa
      721 cattgaagat ggaagcgttc aactagcaga ccattatcaa caaaatactc caattggcga
      781 tggccctgtc cttttaccag acaaccatta cctgtccaca caatctgccc tttcgaaaga
      841 tcccaacgaa aagagagacc acatggtcct tcttgagttt gtaacagctg ctgggattac
      901 acatggcatg gatgaactat acaaataata atactagagc caggcatcaa ataaaacgaa
      961 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct
     1021 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata
//