LOCUS BBa_K836009 3544 bp DNA linear 17-May-2024 DEFINITION Lysis device for R. opacus ACCESSION BBa_K836009 VERSION BBa_K836009.1 FEATURES Location/Qualifiers promoter 1..115 /label=nitA promoter promoter 1..115 /label=BBa_K836008 RBS 124..135 /label=SpoVG misc_feature 136..141 /label=Scar CDS 142..1470 /label=Lysozyme misc_feature 124..1470 /label=BBa_K836005 RBS 1479..1490 /label=SpoVG misc_feature 1491..1496 /label=Spacer CDS 1497..1811 /label=Holin misc_feature 1479..1811 /label=BBa_K836006 misc_feature 1820..1858 /label=B1006 stem_loop 1829..1850 /label=modified thr terminator polyA_site 1851..1858 /label=PolyA polyA_site 1820..1828 /label=PolyA terminator 1820..1858 /label=BBa_B1006 misc_feature 1867..1905 /label=B1006 stem_loop 1876..1897 /label=modified thr terminator polyA_site 1898..1905 /label=PolyA polyA_site 1867..1875 /label=PolyA terminator 1867..1905 /label=BBa_B1006 promoter 1976..1981 /label=Sigma A-35 promoter 1999..2004 /label=Sigma A -10 promoter 1914..2010 /label=BBa_K143012 RBS 2019..2029 /label=BBa_K090506 misc_feature 2030..2035 /label=Scar CDS 2036..2356 /label=Lambda antiholin misc_feature 2019..2356 /label=BBa_K836004 misc_feature 2365..2403 /label=B1006 stem_loop 2374..2395 /label=modified thr terminator polyA_site 2396..2403 /label=PolyA polyA_site 2365..2373 /label=PolyA terminator 2365..2403 /label=BBa_B1006 promoter 2474..2479 /label=Sigma A-35 promoter 2497..2502 /label=Sigma A -10 promoter 2412..2508 /label=BBa_K143012 RBS 2517..2528 /label=SpoVG misc_feature 2529..2534 /label=Spacer CDS 2535..3497 /label=Nitrilase regulator misc_feature 2517..3497 /label=BBa_K836007 misc_feature 3506..3544 /label=B1006 stem_loop 3515..3536 /label=modified thr terminator polyA_site 3537..3544 /label=PolyA polyA_site 3506..3514 /label=PolyA terminator 3506..3544 /label=BBa_B1006 ORIGIN 1 gcgaactccc ttatgcgggt ggcgcagaat gccaggaccc ttgtcattcc acgtcaattc 61 atgcgccttt tcacctcgta ctgtcctgcc aaacacaagc aacggaggta cggactacta 121 gagaaaggtg gtgaatacta gatggcacag cggctctggc cactcccgcg cgacggctac 181 gagctcacct ccggtttcgg accccgctgg ggcgcacagc attccgggct agacctcgcg 241 gccccggacg ggactccgtt ctacgcctgc caggcaggta ccgttcagtt catcggttcc 301 gcgacgggat acggccaatg gctggtgatc gactcgtctg acgacgaggg gtcgggttgt 361 gtcgagtacg gtcacatgtg gaatgcccga gcgaccggcc tctccgtggg agaccacgta 421 gaggccggac agctcatcgg ctacattggg tcgaacggac agtcgaccgg cccccacttg 481 cacatcacgg tttgggaacg tggctataac tcggtccgga tcgacccgga agtctggctg 541 gcaagcgcgg gctttcccgg ggaggcggtc gcggccccga agcccagtac cggcgagccg 601 atctggggaa tcgacgtgtc taaccaccag gggaacttcg acttcgcggc ggcaaaggcg 661 gaggggttcg tgttcgcgac ccataaggtg accgaggcga ccgggtaccg agacccctac 721 tgggcgaggg cccgccagga aatggaaaag catttccccg gccggtgggg aggatacgtg 781 ttctgccgaa cgaatacgga cccggtgacc gaagcgcgat atttctacga cgccgtggga 841 ggtgacacct ccgtgccggt ccaaatcgat tacgaagaca ccaccaacgg aggcagcggt 901 gatgacctct gcaaccgcgt ccgcgcgtat caagacctgg gcttccgcct gctacccatc 961 tatatcccgc gctggtactg gcgagaccac atgggatccc ccgatctctc tggcctgccc 1021 gtaccgatct ggaactcgca ctacgcccgg ggcgtggact acgcctcaaa tctgtacacg 1081 cagcatggcg agaaagccga agcatggtcc gacatgggcg ggaagccggt cagtattctc 1141 cagttttccg agaccgccat ggtagcaggc aaactcatcg acgtgaacgc cttccgcggg 1201 accgaagccg agctcgatga gctcttctcg gggcaagaac tcagcatgag cgccgtgcag 1261 gagattaagg actacattga cgtgcgtgtc accaacccga tcggctcgga cgtcaaggac 1321 ttgagggagc aggcctgcgg ccagggggcc cgcgatgcag gccagtataa cggctggccg 1381 cagttgggga accgcacaat tgccgacgcc ctcgcggtca tcggagttgc tctgaagata 1441 gagggcttct tcgatccgag gagcataggc tactagagaa aggtggtgaa tactagatgc 1501 cagaaaagca cgacctactc gcagccatcc tcgcggcaaa ggagcagggc attggggcga 1561 tcctagcttt cgcgatggcc tacctccggg gccgctacaa cggcggtgcg ttcaccaaga 1621 cagtcatcga cgccactatg tgcgccatca ttgcctggtt catccgagac ctcctggact 1681 tcgctggact ctcgtcgaac ctggcgtaca tcacctcagt cttcatagga tacatcggca 1741 ccgactcaat cgggtctctc atcaagcggt tcgcggccaa gaaggccggt gtcgaggatg 1801 ggcgcaacca atactagaga aaaaaaaacc ccgcccctga cagggcgggg ttttttttta 1861 ctagagaaaa aaaaaccccg cccctgacag ggcggggttt ttttttacta gagaattttg 1921 tcaaaataat tttattgaca acgtcttatt aacgttgata taatttaaat tttatttgac 1981 aaaaatgggc tcgtgttgta caataaatgt tactagagag aggtggtgtt actagatgaa 2041 aatgcctgag aaacatgacc tgctcgccgc aatcctggca gccaaggaac agggcattgg 2101 cgccatcctg gccttcgcga tggcgtatct ccgcggtcgg tacaacggcg gagccttcac 2161 caagaccgtc atcgacgcaa ccatgtgcgc gatcatcgca tggttcatca gagatctcct 2221 ggacttcgcc ggactgtcgt ccaatctcgc ctacatcaca agtgtgttca tcgggtacat 2281 cggcacggac tccatcggct cgctgatcaa gcggttcgcc gccaagaagg cgggggttga 2341 agacggtcgc aaccaatact agagaaaaaa aaaccccgcc cctgacaggg cggggttttt 2401 ttttactaga gaattttgtc aaaataattt tattgacaac gtcttattaa cgttgatata 2461 atttaaattt tatttgacaa aaatgggctc gtgttgtaca ataaatgtta ctagagaaag 2521 gtggtgaata ctagatgaac accttcttct cctccgacca ggtctcggcg cccgaccgcg 2581 tcgcgctctg gcacgacgtc atctgccgca gctacgtccc gctgaacatc accctgacca 2641 gcgagcagcc cttcatcggc accgtctcca ccggcaacct gggcaccgtc cgcatcgcca 2701 cgtcctcgtc cctgccccag cagatcaccc gcacccgccg cctgatccgc caggacgagc 2761 gcgagtacct catggtcggc gtgcagtccg ccggccacgc cctcgtgcag cagcacggcc 2821 gcaccgcccg cgtcggccgc ggcggcctgg tcttctggga cacccgccac ccgtacgaca 2881 tcctcttccc gaccgactgg cgcatgagcg tcttccagtt cccgcgctac tccttcggct 2941 tcaccgagga cttcatcggc cgcatgaccg ccgtcaacgt cggcggcgac cgcggcatcg 3001 gccgcgtggt ctcctccttc atgaccagca tcaacgacgc gaccgacgcc ggcgacctgg 3061 cggaggtcgc ctccctccac aacagcgccg tcgacctgct gtccgcggcg atccggaccg 3121 agctggccga ccaggccgcc gcctccgacg gcctgctcga gtgcgtgctc gcctacatcc 3181 gccagaacct ggccgacccg aacctgtgcg cctcccagat cgccgccgag cacaacgtct 3241 ccgtgcggac cctccaccgc ctgttctcgg ccaccggcca gggcgtggcc gagcacatcc 3301 gcaacctccg cctcgagcgc atcaagaccg agctggccga cccgaccagc cggcgctaca 3361 ccatcagcgc cctggcgcgc aagtggggct tcctcgaccc gtccaccttc tcccgcgcgt 3421 tcaaggacgc ctacggcatc accgcccgcg agtgggcggc ctccgcctcc gcctccccga 3481 ccgaggtctc gtaataatac tagagaaaaa aaaaccccgc ccctgacagg gcggggtttt 3541 tttt //