LOCUS       BBa_K844016             1113 bp    DNA     linear       19-May-2024
DEFINITION  Spider Silk Generator - 4x "B" Silk Construct with His Tag
ACCESSION   BBa_K844016
VERSION     BBa_K844016.1  
FEATURES             Location/Qualifiers
     RBS             209..220
                     /label=BBa_B0034
     promoter        1..200
                     /label=BBa_R0010
     promoter        1..220
                     /label=BBa_K208010
     misc_feature    227..229
                     /label=Start
     misc_feature    230..238
                     /label=Beta-helix
     misc_feature    230..313
                     /label=Elasticity Domain
     misc_feature    239..253
                     /label=Beta-spiral
     misc_feature    260..274
                     /label=Beta-spiral
     misc_feature    275..289
                     /label=Beta-spiral
     misc_feature    298..313
                     /label=Beta-spiral
     misc_feature    314..323
                     /label=Beta-helix
     misc_feature    314..397
                     /label=Elasticity Domain
     misc_feature    324..339
                     /label=Beta-spiral
     misc_feature    343..358
                     /label=Beta-sprial
     misc_feature    359..373
                     /label=Beta-spiral
     misc_feature    382..397
                     /label=Beta-spiral
     misc_feature    398..414
                     /label=Linker Domain
     misc_feature    415..433
                     /label=Strength Domain
     CDS             227..433
                     /label=BBa_K844008
     misc_feature    442..525
                     /label=Elasticity Domain
     misc_feature    442..450
                     /label=Beta-helix
     misc_feature    451..465
                     /label=Beta-spiral
     misc_feature    472..486
                     /label=Beta-spiral
     misc_feature    487..501
                     /label=Beta-spiral
     misc_feature    510..525
                     /label=Beta-spiral
     misc_feature    526..609
                     /label=Elasticity Domain
     misc_feature    526..535
                     /label=Beta-helix
     misc_feature    536..551
                     /label=Beta-spiral
     misc_feature    555..570
                     /label=Beta-spiral
     misc_feature    571..585
                     /label=Beta-spiral
     misc_feature    594..609
                     /label=Beta-spiral
     misc_feature    610..626
                     /label=Linker Domain
     misc_feature    627..645
                     /label=Strength Domain
     CDS             442..645
                     /label=BBa_K844004
     misc_feature    654..737
                     /label=Elasticity Domain
     misc_feature    654..662
                     /label=Beta-helix
     misc_feature    663..677
                     /label=Beta-spiral
     misc_feature    684..698
                     /label=Beta-spiral
     misc_feature    699..713
                     /label=Beta-spiral
     misc_feature    722..737
                     /label=Beta-spiral
     misc_feature    738..821
                     /label=Elasticity Domain
     misc_feature    738..747
                     /label=Beta-helix
     misc_feature    748..763
                     /label=Beta-spiral
     misc_feature    767..782
                     /label=Beta-spiral
     misc_feature    783..797
                     /label=Beta-spiral
     misc_feature    806..821
                     /label=Beta-spiral
     misc_feature    822..838
                     /label=Linker Domain
     misc_feature    839..857
                     /label=Strength Domain
     CDS             654..857
                     /label=BBa_K844004
     misc_feature    866..949
                     /label=Elasticity Domain
     misc_feature    866..874
                     /label=Beta-helix
     misc_feature    875..889
                     /label=Beta-spiral
     misc_feature    896..910
                     /label=Beta-spiral
     misc_feature    911..925
                     /label=Beta-spiral
     misc_feature    934..949
                     /label=Beta-spiral
     misc_feature    950..1033
                     /label=Elasticity Domain
     misc_feature    950..959
                     /label=Beta-helix
     misc_feature    960..975
                     /label=Beta-spiral
     misc_feature    979..994
                     /label=Beta-spiral
     misc_feature    995..1009
                     /label=Beta-spiral
     misc_feature    1018..1033
                     /label=Beta-spiral
     misc_feature    1034..1050
                     /label=Linker Domain
     misc_feature    1051..1069
                     /label=Strength Domain
     CDS             866..1069
                     /label=BBa_K844004
     misc_feature    1078..1107
                     /label=10x-Histidine Tag
     misc_feature    1108..1110
                     /label=Stop
     misc_feature    1111..1113
                     /label=Stop
     misc_feature    1078..1113
                     /label=BBa_K844000
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg gtggatatgg
      241 tcctggagca ggtcaacaag gaccaggtag tcaaggacct ggttctggag gtcaacaagg
      301 accaggtgga caaggtggat atggtcctgg agcaggtcaa caaggaccag gtagtcaagg
      361 acctggttct ggaggtcaac aaggaccagg tggacaaggt ccttatggac caagtgcagc
      421 agcagcagca gcatactaga gggtggatat ggtcctggag caggtcaaca aggaccaggt
      481 agtcaaggac ctggttctgg aggtcaacaa ggaccaggtg gacaaggtgg atatggtcct
      541 ggagcaggtc aacaaggacc aggtagtcaa ggacctggtt ctggaggtca acaaggacca
      601 ggtggacaag gtccttatgg accaagtgca gcagcagcag cagcatacta gagggtggat
      661 atggtcctgg agcaggtcaa caaggaccag gtagtcaagg acctggttct ggaggtcaac
      721 aaggaccagg tggacaaggt ggatatggtc ctggagcagg tcaacaagga ccaggtagtc
      781 aaggacctgg ttctggaggt caacaaggac caggtggaca aggtccttat ggaccaagtg
      841 cagcagcagc agcagcatac tagagggtgg atatggtcct ggagcaggtc aacaaggacc
      901 aggtagtcaa ggacctggtt ctggaggtca acaaggacca ggtggacaag gtggatatgg
      961 tcctggagca ggtcaacaag gaccaggtag tcaaggacct ggttctggag gtcaacaagg
     1021 accaggtgga caaggtcctt atggaccaag tgcagcagca gcagcagcat actagagcat
     1081 catcaccatc accaccatca tcaccattaa taa
//