BBa_B0030 1 BBa_B0030 RBS.1 (strong) -- modified from R. Weiss 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>. false true _44_46_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix (&quot;orig&quot; in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation1701 1 RBS-1\Strong range1701 1 1 15 annotation1702 1 RBS range1702 1 8 12 annotation7025 1 BBa_B0030 range7025 1 1 15 BBa_K861022 1 BBa_K861022 FadE with RBS and terminator 2012-09-05T11:00:00Z 2015-05-08T01:13:35Z E.coli str.K12 lab mutant DH5&#945; FadE was from e.coli DH5&#945;, catalyze the conversion of acyl-CoA to enoyl-CoA. false false _1121_ 0 12357 9 It's complicated false None false Kuanwei Sheng component2181849 1 BBa_B0030 component2181852 1 BBa_B0024 component2181851 1 BBa_K861020 annotation2181851 1 BBa_K861020 range2181851 1 22 2466 annotation2181849 1 BBa_B0030 range2181849 1 1 15 annotation2181852 1 BBa_B0024 range2181852 1 2475 2569 BBa_K861020 1 BBa_K861020 FadE ,acyl-CoA dehydrogenase 2012-09-05T11:00:00Z 2015-05-08T01:13:35Z E.coli str.K12 DH5&#945; The first step in the beta-oxidation pathway involves the conversion of acyl-CoA to enoyl-CoA through acyl-CoA dehydrogenase (FadE). Its action results in the introduction of a trans double-bond between C2 and C3 of the acyl-CoA thioester substrate. And it is repressed by FadR. false false _1121_ 0 12357 9 It's complicated false None false Kuanwei Sheng BBa_K861028 1 BBa_K861028 PfadR regulated FadE 2012-09-20T11:00:00Z 2015-05-08T01:13:35Z E.coli K12 DH5&#945; The first step in the beta-oxidation pathway involves the conversion of acyl-CoA to enoyl-CoA. This process is catalyzed by acyl-CoA dehydrogenase FadE. Its action results in the introduction of a trans double-bond between C2 and C3 of the acyl-CoA thioester substrate. We assebled FadE with PfadR (BBa_K861060) hoping it will only be overexpressed when fatty acids concentration is high. false false _1121_ 0 11007 9 It's complicated false None false Kuanwei Sheng component2190862 1 BBa_K861022 component2190856 1 BBa_K861060 annotation2190862 1 BBa_K861022 range2190862 1 85 2653 annotation2190856 1 BBa_K861060 range2190856 1 1 76 BBa_B0024 1 BBa_B0024 double terminator (B0012-B0011), reversed 2003-12-02T12:00:00Z 2015-08-31T04:07:20Z -- No description -- false false _1_ 0 24 7 In stock false true Caitlin Conboy BBa_K861060 1 PfadR PfadR, synthetic promoter with tandem FadR binding site 2012-09-07T11:00:00Z 2015-05-08T01:13:35Z Escherichia coli str. K-12 substr. MG1655 At least to our knowledge, there is no promoter exists in nature that can respond solely to FadR since those promoters are often regulated by glucose concentration or oxidative stress and many other factors. We decided to design an artificial promoter to fulfill this task. Specifically, a tandem FadR binding site in FadL promoter is place downstream of promoter J23110 with 3 base pair overlap. We test its function in M9 medium with oleic acid as sole carbon source in plasmid pSB6A1. false false _1121_ 0 12357 9 In stock true None false Kuanwei Sheng annotation2183423 1 FadR tandem binding site range2183423 1 33 76 BBa_B0024_sequence 1 aaataataaaaaagccggattaataatctggctttttatattctctctctagtatataaacgcagaaaggcccacccgaaggtgagccagtgtga BBa_K861060_sequence 1 tttacggctagctcagtcctaggtacaatgctagctggtccgacctatactctcgccactggtctgatttctaaga BBa_K861020_sequence 1 atgatgattttgagtattctcgctacggttgtcctgctcggcgcgttgttctatcaccgcgtgagcttatttatcagcagtctgattttgctcgcctggacagcctccctcggcgttgctggtctgtggtcggcgtgggtactggtgcctctggccattatcctcgtgccatttaactttgcgcctatgcgtaagtcgatgatttccgcgccggtatttcgcggtttccgtaaggtgatgccgccgatgtcgcgcactgagaaagaagcgattgatgcgggcaccacctggtgggagggcgacttgttccagggcaagccggactggaaaaagctgcataactatccgcagccgcgcctgaccgccgaagagcaagcgtttctcgacggcccggtagaagaagcctgccggatggcgaatgatttccagatcacccatgagctggcggatctgccgccggagttgtgggcgtaccttaaagagcatcgtttcttcgcgatgatcatcaaaaaagagtacggcgggctggagttctcggcttatgcccagtctcgcgtgctgcaaaaactctccggcgtgagcgggatcctggcgattaccgtcggcgtgccaaactcattaggcccgggcgaactgttgcaacattacggcactgacgagcagaaagatcactatctgccgcgtctggcgcgtggtcaggagatcccctgctttgcactgaccagcccggaagcgggttccgatgcgggcgcgattccggacaccgggattgtctgcatgggcgaatggcagggccagcaggtgctggggatgcgtctgacctggaacaaacgctacattacgctggcaccgattgcgactgtgcttgggctggcgtttaaactctccgacccggaaaagctgctgggtggcgcagaagatttaggtattacctgtgcgctgatcccgaccaccacgccgggcgtggaaattggtcgtcgccacttcccgctgaacgtaccgttccagaacggaccgacgcgcggtaaagatgtcttcgtaccgatcgattacatcatcggtgggccgaaaatggccgggcaaggctggcggatgctggtggagtgcctctcggtaggccgcggcatcaccctgccttccaactcaaccggcggcgtgaaatcggtagcgctggcaacgggcgcgtatgctcacattcgccgtcagttcaaaatctctatcggtaagatggaagggattgaagagccgctggcgcgtattgccggtaatgcctacgtgatggatgctgcggcatcgctgattacctacggcattatgctcggcgaaaaacctgccgtgctgtcggctatcgttaagtatcactgtacccaccgcgggcagcagtcgattattgatgcgatggatatcactggtggcaaaggcattatgctcgggcagagcaacttcctggcgcgtgcttaccagggcgcaccgattgccatcaccgttgaaggggccaacattctgacccgcagcatgatgatcttcggacaaggagcgattcgttgccatccgtacgtgctggaagagatggaagcagcgaagaacaatgacgtcaacgcgttcgataaactgttgttcaaacatatcggtcacgtcggtagcaacaaagttcgcagcttctggctgggcctgacgcgcggtttaaccagcagcacgccaaccggcgatgccactaaacgctactatcagcacctgaaccgcctgagcgccaacctcgccctgctttctgatgtctcgatggctgtgctgggcggcagcctgaaacgtcgtgagcgcatctcggcacgtctgggggatattttaagccagctttacctcgcctctgccgttctgaagcgttatgacgacgaaggccgtaatgaagccgacctgccgctggtgcactggggcgtacaagatgcgctgtatcaggctgaacaggcgatggatgatttactgcaaaacttcccgaaccgcgtggttgccgggctgctgaatgtggtgatcttcccgaccggacgtcattatctggcaccttctgacaagctggatcataaagtggcgaagattttacaagtgccgaacgccacccgttcccgcattggtcgcggtcagtacctgacgccgagcgagcataatccggttggcttgctggaagaggcgctggtggatgtgattgccgccgacccaattcatcagcggatctgtaaagagctgggtaaaaacctgccgtttacccgtctggatgaactggcgcacaacgcgctggcgaaggggctgattgataaagatgaagccgctattctggtgaaagctgaagaaagccgtctgcgcagtattaacgttgatgactttgatccggaagagctggcgacgaagccggtaaagttgccggagaaagtgcggaaagttgaagccgcgtaa BBa_K861028_sequence 1 tttacggctagctcagtcctaggtacaatgctagctggtccgacctatactctcgccactggtctgatttctaagatactagagattaaagaggagaaatactagatgatgattttgagtattctcgctacggttgtcctgctcggcgcgttgttctatcaccgcgtgagcttatttatcagcagtctgattttgctcgcctggacagcctccctcggcgttgctggtctgtggtcggcgtgggtactggtgcctctggccattatcctcgtgccatttaactttgcgcctatgcgtaagtcgatgatttccgcgccggtatttcgcggtttccgtaaggtgatgccgccgatgtcgcgcactgagaaagaagcgattgatgcgggcaccacctggtgggagggcgacttgttccagggcaagccggactggaaaaagctgcataactatccgcagccgcgcctgaccgccgaagagcaagcgtttctcgacggcccggtagaagaagcctgccggatggcgaatgatttccagatcacccatgagctggcggatctgccgccggagttgtgggcgtaccttaaagagcatcgtttcttcgcgatgatcatcaaaaaagagtacggcgggctggagttctcggcttatgcccagtctcgcgtgctgcaaaaactctccggcgtgagcgggatcctggcgattaccgtcggcgtgccaaactcattaggcccgggcgaactgttgcaacattacggcactgacgagcagaaagatcactatctgccgcgtctggcgcgtggtcaggagatcccctgctttgcactgaccagcccggaagcgggttccgatgcgggcgcgattccggacaccgggattgtctgcatgggcgaatggcagggccagcaggtgctggggatgcgtctgacctggaacaaacgctacattacgctggcaccgattgcgactgtgcttgggctggcgtttaaactctccgacccggaaaagctgctgggtggcgcagaagatttaggtattacctgtgcgctgatcccgaccaccacgccgggcgtggaaattggtcgtcgccacttcccgctgaacgtaccgttccagaacggaccgacgcgcggtaaagatgtcttcgtaccgatcgattacatcatcggtgggccgaaaatggccgggcaaggctggcggatgctggtggagtgcctctcggtaggccgcggcatcaccctgccttccaactcaaccggcggcgtgaaatcggtagcgctggcaacgggcgcgtatgctcacattcgccgtcagttcaaaatctctatcggtaagatggaagggattgaagagccgctggcgcgtattgccggtaatgcctacgtgatggatgctgcggcatcgctgattacctacggcattatgctcggcgaaaaacctgccgtgctgtcggctatcgttaagtatcactgtacccaccgcgggcagcagtcgattattgatgcgatggatatcactggtggcaaaggcattatgctcgggcagagcaacttcctggcgcgtgcttaccagggcgcaccgattgccatcaccgttgaaggggccaacattctgacccgcagcatgatgatcttcggacaaggagcgattcgttgccatccgtacgtgctggaagagatggaagcagcgaagaacaatgacgtcaacgcgttcgataaactgttgttcaaacatatcggtcacgtcggtagcaacaaagttcgcagcttctggctgggcctgacgcgcggtttaaccagcagcacgccaaccggcgatgccactaaacgctactatcagcacctgaaccgcctgagcgccaacctcgccctgctttctgatgtctcgatggctgtgctgggcggcagcctgaaacgtcgtgagcgcatctcggcacgtctgggggatattttaagccagctttacctcgcctctgccgttctgaagcgttatgacgacgaaggccgtaatgaagccgacctgccgctggtgcactggggcgtacaagatgcgctgtatcaggctgaacaggcgatggatgatttactgcaaaacttcccgaaccgcgtggttgccgggctgctgaatgtggtgatcttcccgaccggacgtcattatctggcaccttctgacaagctggatcataaagtggcgaagattttacaagtgccgaacgccacccgttcccgcattggtcgcggtcagtacctgacgccgagcgagcataatccggttggcttgctggaagaggcgctggtggatgtgattgccgccgacccaattcatcagcggatctgtaaagagctgggtaaaaacctgccgtttacccgtctggatgaactggcgcacaacgcgctggcgaaggggctgattgataaagatgaagccgctattctggtgaaagctgaagaaagccgtctgcgcagtattaacgttgatgactttgatccggaagagctggcgacgaagccggtaaagttgccggagaaagtgcggaaagttgaagccgcgtaatactagagaaataataaaaaagccggattaataatctggctttttatattctctctctagtatataaacgcagaaaggcccacccgaaggtgagccagtgtga BBa_K861022_sequence 1 attaaagaggagaaatactagatgatgattttgagtattctcgctacggttgtcctgctcggcgcgttgttctatcaccgcgtgagcttatttatcagcagtctgattttgctcgcctggacagcctccctcggcgttgctggtctgtggtcggcgtgggtactggtgcctctggccattatcctcgtgccatttaactttgcgcctatgcgtaagtcgatgatttccgcgccggtatttcgcggtttccgtaaggtgatgccgccgatgtcgcgcactgagaaagaagcgattgatgcgggcaccacctggtgggagggcgacttgttccagggcaagccggactggaaaaagctgcataactatccgcagccgcgcctgaccgccgaagagcaagcgtttctcgacggcccggtagaagaagcctgccggatggcgaatgatttccagatcacccatgagctggcggatctgccgccggagttgtgggcgtaccttaaagagcatcgtttcttcgcgatgatcatcaaaaaagagtacggcgggctggagttctcggcttatgcccagtctcgcgtgctgcaaaaactctccggcgtgagcgggatcctggcgattaccgtcggcgtgccaaactcattaggcccgggcgaactgttgcaacattacggcactgacgagcagaaagatcactatctgccgcgtctggcgcgtggtcaggagatcccctgctttgcactgaccagcccggaagcgggttccgatgcgggcgcgattccggacaccgggattgtctgcatgggcgaatggcagggccagcaggtgctggggatgcgtctgacctggaacaaacgctacattacgctggcaccgattgcgactgtgcttgggctggcgtttaaactctccgacccggaaaagctgctgggtggcgcagaagatttaggtattacctgtgcgctgatcccgaccaccacgccgggcgtggaaattggtcgtcgccacttcccgctgaacgtaccgttccagaacggaccgacgcgcggtaaagatgtcttcgtaccgatcgattacatcatcggtgggccgaaaatggccgggcaaggctggcggatgctggtggagtgcctctcggtaggccgcggcatcaccctgccttccaactcaaccggcggcgtgaaatcggtagcgctggcaacgggcgcgtatgctcacattcgccgtcagttcaaaatctctatcggtaagatggaagggattgaagagccgctggcgcgtattgccggtaatgcctacgtgatggatgctgcggcatcgctgattacctacggcattatgctcggcgaaaaacctgccgtgctgtcggctatcgttaagtatcactgtacccaccgcgggcagcagtcgattattgatgcgatggatatcactggtggcaaaggcattatgctcgggcagagcaacttcctggcgcgtgcttaccagggcgcaccgattgccatcaccgttgaaggggccaacattctgacccgcagcatgatgatcttcggacaaggagcgattcgttgccatccgtacgtgctggaagagatggaagcagcgaagaacaatgacgtcaacgcgttcgataaactgttgttcaaacatatcggtcacgtcggtagcaacaaagttcgcagcttctggctgggcctgacgcgcggtttaaccagcagcacgccaaccggcgatgccactaaacgctactatcagcacctgaaccgcctgagcgccaacctcgccctgctttctgatgtctcgatggctgtgctgggcggcagcctgaaacgtcgtgagcgcatctcggcacgtctgggggatattttaagccagctttacctcgcctctgccgttctgaagcgttatgacgacgaaggccgtaatgaagccgacctgccgctggtgcactggggcgtacaagatgcgctgtatcaggctgaacaggcgatggatgatttactgcaaaacttcccgaaccgcgtggttgccgggctgctgaatgtggtgatcttcccgaccggacgtcattatctggcaccttctgacaagctggatcataaagtggcgaagattttacaagtgccgaacgccacccgttcccgcattggtcgcggtcagtacctgacgccgagcgagcataatccggttggcttgctggaagaggcgctggtggatgtgattgccgccgacccaattcatcagcggatctgtaaagagctgggtaaaaacctgccgtttacccgtctggatgaactggcgcacaacgcgctggcgaaggggctgattgataaagatgaagccgctattctggtgaaagctgaagaaagccgtctgcgcagtattaacgttgatgactttgatccggaagagctggcgacgaagccggtaaagttgccggagaaagtgcggaaagttgaagccgcgtaatactagagaaataataaaaaagccggattaataatctggctttttatattctctctctagtatataaacgcagaaaggcccacccgaaggtgagccagtgtga BBa_B0030_sequence 1 attaaagaggagaaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z