BBa_B0030
1
BBa_B0030
RBS.1 (strong) -- modified from R. Weiss
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_44_46_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation1701
1
RBS-1\Strong
range1701
1
1
15
annotation1702
1
RBS
range1702
1
8
12
annotation7025
1
BBa_B0030
range7025
1
1
15
BBa_K861022
1
BBa_K861022
FadE with RBS and terminator
2012-09-05T11:00:00Z
2015-05-08T01:13:35Z
E.coli str.K12 lab mutant DH5α
FadE was from e.coli DH5α, catalyze the conversion of acyl-CoA to enoyl-CoA.
false
false
_1121_
0
12357
9
It's complicated
false
None
false
Kuanwei Sheng
component2181849
1
BBa_B0030
component2181852
1
BBa_B0024
component2181851
1
BBa_K861020
annotation2181851
1
BBa_K861020
range2181851
1
22
2466
annotation2181849
1
BBa_B0030
range2181849
1
1
15
annotation2181852
1
BBa_B0024
range2181852
1
2475
2569
BBa_K861020
1
BBa_K861020
FadE ,acyl-CoA dehydrogenase
2012-09-05T11:00:00Z
2015-05-08T01:13:35Z
E.coli str.K12 DH5α
The first step in the beta-oxidation pathway involves the conversion of acyl-CoA to enoyl-CoA through acyl-CoA dehydrogenase (FadE). Its action results in the introduction of a trans double-bond between C2 and C3 of the acyl-CoA thioester substrate. And it is repressed by FadR.
false
false
_1121_
0
12357
9
It's complicated
false
None
false
Kuanwei Sheng
BBa_K861028
1
BBa_K861028
PfadR regulated FadE
2012-09-20T11:00:00Z
2015-05-08T01:13:35Z
E.coli K12 DH5α
The first step in the beta-oxidation pathway involves the conversion of acyl-CoA to enoyl-CoA. This process is catalyzed by acyl-CoA dehydrogenase FadE. Its action results in the introduction of a trans double-bond between C2 and C3 of the acyl-CoA thioester substrate. We assebled FadE with PfadR (BBa_K861060) hoping it will only be overexpressed when fatty acids concentration is high.
false
false
_1121_
0
11007
9
It's complicated
false
None
false
Kuanwei Sheng
component2190862
1
BBa_K861022
component2190856
1
BBa_K861060
annotation2190862
1
BBa_K861022
range2190862
1
85
2653
annotation2190856
1
BBa_K861060
range2190856
1
1
76
BBa_B0024
1
BBa_B0024
double terminator (B0012-B0011), reversed
2003-12-02T12:00:00Z
2015-08-31T04:07:20Z
-- No description --
false
false
_1_
0
24
7
In stock
false
true
Caitlin Conboy
BBa_K861060
1
PfadR
PfadR, synthetic promoter with tandem FadR binding site
2012-09-07T11:00:00Z
2015-05-08T01:13:35Z
Escherichia coli str. K-12 substr. MG1655
At least to our knowledge, there is no promoter exists in nature that can respond solely to FadR since those promoters are often regulated by glucose concentration or oxidative stress and many other factors. We decided to design an artificial promoter to fulfill this task. Specifically, a tandem FadR binding site in FadL promoter is place downstream of promoter J23110 with 3 base pair overlap.
We test its function in M9 medium with oleic acid as sole carbon source in plasmid pSB6A1.
false
false
_1121_
0
12357
9
In stock
true
None
false
Kuanwei Sheng
annotation2183423
1
FadR tandem binding site
range2183423
1
33
76
BBa_B0024_sequence
1
aaataataaaaaagccggattaataatctggctttttatattctctctctagtatataaacgcagaaaggcccacccgaaggtgagccagtgtga
BBa_K861060_sequence
1
tttacggctagctcagtcctaggtacaatgctagctggtccgacctatactctcgccactggtctgatttctaaga
BBa_K861020_sequence
1
atgatgattttgagtattctcgctacggttgtcctgctcggcgcgttgttctatcaccgcgtgagcttatttatcagcagtctgattttgctcgcctggacagcctccctcggcgttgctggtctgtggtcggcgtgggtactggtgcctctggccattatcctcgtgccatttaactttgcgcctatgcgtaagtcgatgatttccgcgccggtatttcgcggtttccgtaaggtgatgccgccgatgtcgcgcactgagaaagaagcgattgatgcgggcaccacctggtgggagggcgacttgttccagggcaagccggactggaaaaagctgcataactatccgcagccgcgcctgaccgccgaagagcaagcgtttctcgacggcccggtagaagaagcctgccggatggcgaatgatttccagatcacccatgagctggcggatctgccgccggagttgtgggcgtaccttaaagagcatcgtttcttcgcgatgatcatcaaaaaagagtacggcgggctggagttctcggcttatgcccagtctcgcgtgctgcaaaaactctccggcgtgagcgggatcctggcgattaccgtcggcgtgccaaactcattaggcccgggcgaactgttgcaacattacggcactgacgagcagaaagatcactatctgccgcgtctggcgcgtggtcaggagatcccctgctttgcactgaccagcccggaagcgggttccgatgcgggcgcgattccggacaccgggattgtctgcatgggcgaatggcagggccagcaggtgctggggatgcgtctgacctggaacaaacgctacattacgctggcaccgattgcgactgtgcttgggctggcgtttaaactctccgacccggaaaagctgctgggtggcgcagaagatttaggtattacctgtgcgctgatcccgaccaccacgccgggcgtggaaattggtcgtcgccacttcccgctgaacgtaccgttccagaacggaccgacgcgcggtaaagatgtcttcgtaccgatcgattacatcatcggtgggccgaaaatggccgggcaaggctggcggatgctggtggagtgcctctcggtaggccgcggcatcaccctgccttccaactcaaccggcggcgtgaaatcggtagcgctggcaacgggcgcgtatgctcacattcgccgtcagttcaaaatctctatcggtaagatggaagggattgaagagccgctggcgcgtattgccggtaatgcctacgtgatggatgctgcggcatcgctgattacctacggcattatgctcggcgaaaaacctgccgtgctgtcggctatcgttaagtatcactgtacccaccgcgggcagcagtcgattattgatgcgatggatatcactggtggcaaaggcattatgctcgggcagagcaacttcctggcgcgtgcttaccagggcgcaccgattgccatcaccgttgaaggggccaacattctgacccgcagcatgatgatcttcggacaaggagcgattcgttgccatccgtacgtgctggaagagatggaagcagcgaagaacaatgacgtcaacgcgttcgataaactgttgttcaaacatatcggtcacgtcggtagcaacaaagttcgcagcttctggctgggcctgacgcgcggtttaaccagcagcacgccaaccggcgatgccactaaacgctactatcagcacctgaaccgcctgagcgccaacctcgccctgctttctgatgtctcgatggctgtgctgggcggcagcctgaaacgtcgtgagcgcatctcggcacgtctgggggatattttaagccagctttacctcgcctctgccgttctgaagcgttatgacgacgaaggccgtaatgaagccgacctgccgctggtgcactggggcgtacaagatgcgctgtatcaggctgaacaggcgatggatgatttactgcaaaacttcccgaaccgcgtggttgccgggctgctgaatgtggtgatcttcccgaccggacgtcattatctggcaccttctgacaagctggatcataaagtggcgaagattttacaagtgccgaacgccacccgttcccgcattggtcgcggtcagtacctgacgccgagcgagcataatccggttggcttgctggaagaggcgctggtggatgtgattgccgccgacccaattcatcagcggatctgtaaagagctgggtaaaaacctgccgtttacccgtctggatgaactggcgcacaacgcgctggcgaaggggctgattgataaagatgaagccgctattctggtgaaagctgaagaaagccgtctgcgcagtattaacgttgatgactttgatccggaagagctggcgacgaagccggtaaagttgccggagaaagtgcggaaagttgaagccgcgtaa
BBa_K861028_sequence
1
tttacggctagctcagtcctaggtacaatgctagctggtccgacctatactctcgccactggtctgatttctaagatactagagattaaagaggagaaatactagatgatgattttgagtattctcgctacggttgtcctgctcggcgcgttgttctatcaccgcgtgagcttatttatcagcagtctgattttgctcgcctggacagcctccctcggcgttgctggtctgtggtcggcgtgggtactggtgcctctggccattatcctcgtgccatttaactttgcgcctatgcgtaagtcgatgatttccgcgccggtatttcgcggtttccgtaaggtgatgccgccgatgtcgcgcactgagaaagaagcgattgatgcgggcaccacctggtgggagggcgacttgttccagggcaagccggactggaaaaagctgcataactatccgcagccgcgcctgaccgccgaagagcaagcgtttctcgacggcccggtagaagaagcctgccggatggcgaatgatttccagatcacccatgagctggcggatctgccgccggagttgtgggcgtaccttaaagagcatcgtttcttcgcgatgatcatcaaaaaagagtacggcgggctggagttctcggcttatgcccagtctcgcgtgctgcaaaaactctccggcgtgagcgggatcctggcgattaccgtcggcgtgccaaactcattaggcccgggcgaactgttgcaacattacggcactgacgagcagaaagatcactatctgccgcgtctggcgcgtggtcaggagatcccctgctttgcactgaccagcccggaagcgggttccgatgcgggcgcgattccggacaccgggattgtctgcatgggcgaatggcagggccagcaggtgctggggatgcgtctgacctggaacaaacgctacattacgctggcaccgattgcgactgtgcttgggctggcgtttaaactctccgacccggaaaagctgctgggtggcgcagaagatttaggtattacctgtgcgctgatcccgaccaccacgccgggcgtggaaattggtcgtcgccacttcccgctgaacgtaccgttccagaacggaccgacgcgcggtaaagatgtcttcgtaccgatcgattacatcatcggtgggccgaaaatggccgggcaaggctggcggatgctggtggagtgcctctcggtaggccgcggcatcaccctgccttccaactcaaccggcggcgtgaaatcggtagcgctggcaacgggcgcgtatgctcacattcgccgtcagttcaaaatctctatcggtaagatggaagggattgaagagccgctggcgcgtattgccggtaatgcctacgtgatggatgctgcggcatcgctgattacctacggcattatgctcggcgaaaaacctgccgtgctgtcggctatcgttaagtatcactgtacccaccgcgggcagcagtcgattattgatgcgatggatatcactggtggcaaaggcattatgctcgggcagagcaacttcctggcgcgtgcttaccagggcgcaccgattgccatcaccgttgaaggggccaacattctgacccgcagcatgatgatcttcggacaaggagcgattcgttgccatccgtacgtgctggaagagatggaagcagcgaagaacaatgacgtcaacgcgttcgataaactgttgttcaaacatatcggtcacgtcggtagcaacaaagttcgcagcttctggctgggcctgacgcgcggtttaaccagcagcacgccaaccggcgatgccactaaacgctactatcagcacctgaaccgcctgagcgccaacctcgccctgctttctgatgtctcgatggctgtgctgggcggcagcctgaaacgtcgtgagcgcatctcggcacgtctgggggatattttaagccagctttacctcgcctctgccgttctgaagcgttatgacgacgaaggccgtaatgaagccgacctgccgctggtgcactggggcgtacaagatgcgctgtatcaggctgaacaggcgatggatgatttactgcaaaacttcccgaaccgcgtggttgccgggctgctgaatgtggtgatcttcccgaccggacgtcattatctggcaccttctgacaagctggatcataaagtggcgaagattttacaagtgccgaacgccacccgttcccgcattggtcgcggtcagtacctgacgccgagcgagcataatccggttggcttgctggaagaggcgctggtggatgtgattgccgccgacccaattcatcagcggatctgtaaagagctgggtaaaaacctgccgtttacccgtctggatgaactggcgcacaacgcgctggcgaaggggctgattgataaagatgaagccgctattctggtgaaagctgaagaaagccgtctgcgcagtattaacgttgatgactttgatccggaagagctggcgacgaagccggtaaagttgccggagaaagtgcggaaagttgaagccgcgtaatactagagaaataataaaaaagccggattaataatctggctttttatattctctctctagtatataaacgcagaaaggcccacccgaaggtgagccagtgtga
BBa_K861022_sequence
1
attaaagaggagaaatactagatgatgattttgagtattctcgctacggttgtcctgctcggcgcgttgttctatcaccgcgtgagcttatttatcagcagtctgattttgctcgcctggacagcctccctcggcgttgctggtctgtggtcggcgtgggtactggtgcctctggccattatcctcgtgccatttaactttgcgcctatgcgtaagtcgatgatttccgcgccggtatttcgcggtttccgtaaggtgatgccgccgatgtcgcgcactgagaaagaagcgattgatgcgggcaccacctggtgggagggcgacttgttccagggcaagccggactggaaaaagctgcataactatccgcagccgcgcctgaccgccgaagagcaagcgtttctcgacggcccggtagaagaagcctgccggatggcgaatgatttccagatcacccatgagctggcggatctgccgccggagttgtgggcgtaccttaaagagcatcgtttcttcgcgatgatcatcaaaaaagagtacggcgggctggagttctcggcttatgcccagtctcgcgtgctgcaaaaactctccggcgtgagcgggatcctggcgattaccgtcggcgtgccaaactcattaggcccgggcgaactgttgcaacattacggcactgacgagcagaaagatcactatctgccgcgtctggcgcgtggtcaggagatcccctgctttgcactgaccagcccggaagcgggttccgatgcgggcgcgattccggacaccgggattgtctgcatgggcgaatggcagggccagcaggtgctggggatgcgtctgacctggaacaaacgctacattacgctggcaccgattgcgactgtgcttgggctggcgtttaaactctccgacccggaaaagctgctgggtggcgcagaagatttaggtattacctgtgcgctgatcccgaccaccacgccgggcgtggaaattggtcgtcgccacttcccgctgaacgtaccgttccagaacggaccgacgcgcggtaaagatgtcttcgtaccgatcgattacatcatcggtgggccgaaaatggccgggcaaggctggcggatgctggtggagtgcctctcggtaggccgcggcatcaccctgccttccaactcaaccggcggcgtgaaatcggtagcgctggcaacgggcgcgtatgctcacattcgccgtcagttcaaaatctctatcggtaagatggaagggattgaagagccgctggcgcgtattgccggtaatgcctacgtgatggatgctgcggcatcgctgattacctacggcattatgctcggcgaaaaacctgccgtgctgtcggctatcgttaagtatcactgtacccaccgcgggcagcagtcgattattgatgcgatggatatcactggtggcaaaggcattatgctcgggcagagcaacttcctggcgcgtgcttaccagggcgcaccgattgccatcaccgttgaaggggccaacattctgacccgcagcatgatgatcttcggacaaggagcgattcgttgccatccgtacgtgctggaagagatggaagcagcgaagaacaatgacgtcaacgcgttcgataaactgttgttcaaacatatcggtcacgtcggtagcaacaaagttcgcagcttctggctgggcctgacgcgcggtttaaccagcagcacgccaaccggcgatgccactaaacgctactatcagcacctgaaccgcctgagcgccaacctcgccctgctttctgatgtctcgatggctgtgctgggcggcagcctgaaacgtcgtgagcgcatctcggcacgtctgggggatattttaagccagctttacctcgcctctgccgttctgaagcgttatgacgacgaaggccgtaatgaagccgacctgccgctggtgcactggggcgtacaagatgcgctgtatcaggctgaacaggcgatggatgatttactgcaaaacttcccgaaccgcgtggttgccgggctgctgaatgtggtgatcttcccgaccggacgtcattatctggcaccttctgacaagctggatcataaagtggcgaagattttacaagtgccgaacgccacccgttcccgcattggtcgcggtcagtacctgacgccgagcgagcataatccggttggcttgctggaagaggcgctggtggatgtgattgccgccgacccaattcatcagcggatctgtaaagagctgggtaaaaacctgccgtttacccgtctggatgaactggcgcacaacgcgctggcgaaggggctgattgataaagatgaagccgctattctggtgaaagctgaagaaagccgtctgcgcagtattaacgttgatgactttgatccggaagagctggcgacgaagccggtaaagttgccggagaaagtgcggaaagttgaagccgcgtaatactagagaaataataaaaaagccggattaataatctggctttttatattctctctctagtatataaacgcagaaaggcccacccgaaggtgagccagtgtga
BBa_B0030_sequence
1
attaaagaggagaaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z