LOCUS       BBa_K861175              905 bp    DNA     linear       27-May-2024
DEFINITION  Efficacy testing RFP generator of BBa_K861170 (PI)
ACCESSION   BBa_K861175
VERSION     BBa_K861175.1  
FEATURES             Location/Qualifiers
     misc_feature    15..36
                     /label=Modified Consensus Crp binding site
     promoter        1..36
                     /label=BBa_K861170
     misc_feature    49..52
                     /label=conserved
     RBS             45..56
                     /label=BBa_B0034
     CDS             63..737
                     /label=mrfp1
     misc_feature    744..768
                     /label=Help:Barcodes
     CDS             63..743
                     /label=BBa_E1010
     stem_loop       788..831
                     /label=stem_loop
     misc_feature    777..856
                     /label=BBa_B0010
     terminator      777..856
                     /label=BBa_B0010
     stem_loop       872..891
                     /label=T7 TE
     misc_feature    898..898
                     /label=stop
     polyA_site      892..905
                     /label=polya
     misc_feature    865..905
                     /label=BBa_B0012
     terminator      865..905
                     /label=BBa_B0012
     misc_feature    45..905
                     /label=BBa_I13507
ORIGIN
        1 ttgacagcta gctcaaatgt gattataatc acattttact agagaaagag gagaaatact
       61 agatggcttc ctccgaagac gttatcaaag agttcatgcg tttcaaagtt cgtatggaag
      121 gttccgttaa cggtcacgag ttcgaaatcg aaggtgaagg tgaaggtcgt ccgtacgaag
      181 gtacccagac cgctaaactg aaagttacca aaggtggtcc gctgccgttc gcttgggaca
      241 tcctgtcccc gcagttccag tacggttcca aagcttacgt taaacacccg gctgacatcc
      301 cggactacct gaaactgtcc ttcccggaag gtttcaaatg ggaacgtgtt atgaacttcg
      361 aagacggtgg tgttgttacc gttacccagg actcctccct gcaagacggt gagttcatct
      421 acaaagttaa actgcgtggt accaacttcc cgtccgacgg tccggttatg cagaaaaaaa
      481 ccatgggttg ggaagcttcc accgaacgta tgtacccgga agacggtgct ctgaaaggtg
      541 aaatcaaaat gcgtctgaaa ctgaaagacg gtggtcacta cgacgctgaa gttaaaacca
      601 cctacatggc taaaaaaccg gttcagctgc cgggtgctta caaaaccgac atcaaactgg
      661 acatcacctc ccacaacgaa gactacacca tcgttgaaca gtacgaacgt gctgaaggtc
      721 gtcactccac cggtgcttaa taacgctgat agtgctagtg tagatcgcta ctagagccag
      781 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
      841 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt
      901 ttata
//