BBa_K861041 1 BBa_K861041 FadI, gene for fatty acid degradation from <i>E.coli str. K12</i> 2012-09-05T11:00:00Z 2015-05-08T01:13:35Z E.coli str.K12 lab mutant DH5&#945; FadI is the anaerobic counterpart of FadA. It is shown to have better degradation of medium and short length fatty acid together with FadJ false false _1121_ 0 12357 9 It's complicated false None false Kuanwei Sheng BBa_K861347 1 BBa_K861347 IPTG induced promoter with FadI, intermediate part for BBa_K861037 2012-09-20T11:00:00Z 2015-05-08T01:13:36Z E.coli K12 DH5&#945; To find the optimal combination of those fatty acid degradation enzymes, we used IPTG induced promoter BBa_R0011 to express and extract those proteins, put them in different combination with substrates needed for fatty acid oxidation in a test tube to look for the combination that have best degradation capability. This is a intermediate part for BBa_K861037. false false _1121_ 0 11007 9 It's complicated false None false Kuanwei Sheng component2191310 1 BBa_K861044 component2191301 1 BBa_R0011 annotation2191301 1 BBa_R0011 range2191301 1 1 54 annotation2191310 1 BBa_K861044 range2191310 1 64 1395 BBa_K861044 1 BBa_K861044 FadI with a RBS 2012-09-05T11:00:00Z 2015-05-08T01:13:35Z E.coli str.K12 lab mutant DH5&#945; FadI is the anaerobic counterpart of FadA. It is shown to have better degradation of medium and short length fatty acid together with FadJ.This device contains FadI with a RBS and a terminator. false false _1121_ 0 12357 9 It's complicated false None false Kuanwei Sheng component2182628 1 BBa_K861041 component2182626 1 BBa_B0030 annotation2182626 1 BBa_B0030 range2182626 1 1 15 annotation2182628 1 BBa_K861041 range2182628 1 22 1332 BBa_R0011 1 lacI+pL Promoter (lacI regulated, lambda pL hybrid) 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z represillator of Elowitz and Leibler (2000) Released HQ 2013 Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bb_part>BBa_C0010</bb_part>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference). false true _1_ 0 24 7 In stock false <P> <P>hybrid promoter design to create strong promoter that is, at the same time, highly repressible. note that the upstream operator installed in this hybrid is slightly different than the one in the original source (Lutz and Bujard, 1997). the most upstream operator region is slightly truncated in the represillator version, so that both operators in the hybrid are the same sequence. see references for details. also, the sequence has been truncated after the transcriptional start site.<P>LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and increase transcription. This part is incompatible with environments containing lactose or lactose analogs. true Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton annotation2001 1 lac O1 range2001 1 26 42 annotation7064 1 BBa_R0011 range7064 1 1 54 annotation1999 1 lac O1 range1999 1 3 19 annotation2000 1 -35 range2000 1 20 25 annotation2002 1 -10 range2002 1 43 48 BBa_B0030 1 BBa_B0030 RBS.1 (strong) -- modified from R. Weiss 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>. false true _44_46_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix (&quot;orig&quot; in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation7025 1 BBa_B0030 range7025 1 1 15 annotation1702 1 RBS range1702 1 8 12 annotation1701 1 RBS-1\Strong range1701 1 1 15 BBa_K861347_sequence 1 aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagattaaagaggagaaatactagatgggtcaggttttaccgctggttacccgccagggcgatcgtatcgccattgttagcggtttacgtacgccttttgcccgtcaggcgacggcttttcatggcattcccgcggttgatttagggaagatggtggtaggcgaactgctggcacgcagcgagatccccgccgaagtgattgaacaactggtctttggtcaggtcgtacaaatgcctgaagcccccaacattgcgcgtgaaattgttctcggtacgggaatgaatgtacataccgatgcttacagcgtcagccgcgcttgcgctaccagtttccaggcagttgcaaacgtcgcagaaagcctgatggcgggaactattcgagcggggattgccggtggggcagattcctcttcggtattgccaattggcgtcagtaaaaaactggcgcgcgtgctggttgatgtcaacaaagctcgtaccatgagccagcgactgaaactcttctctcgcctgcgtttgcgcgacttaatgcccgtaccacctgcggtagcagaatattctaccggcttgcggatgggcgacaccgcagagcaaatggcgaaaacctacggcatcacccgagaacagcaagatgcattagcgcaccgttcgcatcagcgtgccgctcaggcatggtcagacggaaaactcaaagaagaggtgatgactgcctttatccctccttataaacaaccgcttgtcgaagacaacaatattcgcggtaattcctcgcttgccgattacgcaaagctgcgcccggcgtttgatcgcaaacacggaacggtaacggcggcaaacagtacgccgctgaccgatggcgcggcagcggtgatcctgatgactgaatcccgggcgaaagaattagggctggtgccgctggggtatctgcgcagctacgcatttactgcgattgatgtctggcaggacatgttgctcggtccagcctggtcaacaccgctggcgctggagcgtgccggtttgacgatgagcgatctgacattgatcgatatgcacgaagcctttgcagctcagacgctggcgaatattcagttgctgggtagtgaacgttttgctcgtgaagcactggggcgtgcacatgccactggcgaagtggacgatagcaaatttaacgtgcttggcggttcgattgcttacgggcatcccttcgcggcgaccggcgcgcggatgattacccagacattgcatgaacttcgccgtcgcggcggtggatttggtttagttaccgcctgtgctgccggtgggcttggcgcggcaatggttctggaggcggaataa BBa_K861041_sequence 1 atgggtcaggttttaccgctggttacccgccagggcgatcgtatcgccattgttagcggtttacgtacgccttttgcccgtcaggcgacggcttttcatggcattcccgcggttgatttagggaagatggtggtaggcgaactgctggcacgcagcgagatccccgccgaagtgattgaacaactggtctttggtcaggtcgtacaaatgcctgaagcccccaacattgcgcgtgaaattgttctcggtacgggaatgaatgtacataccgatgcttacagcgtcagccgcgcttgcgctaccagtttccaggcagttgcaaacgtcgcagaaagcctgatggcgggaactattcgagcggggattgccggtggggcagattcctcttcggtattgccaattggcgtcagtaaaaaactggcgcgcgtgctggttgatgtcaacaaagctcgtaccatgagccagcgactgaaactcttctctcgcctgcgtttgcgcgacttaatgcccgtaccacctgcggtagcagaatattctaccggcttgcggatgggcgacaccgcagagcaaatggcgaaaacctacggcatcacccgagaacagcaagatgcattagcgcaccgttcgcatcagcgtgccgctcaggcatggtcagacggaaaactcaaagaagaggtgatgactgcctttatccctccttataaacaaccgcttgtcgaagacaacaatattcgcggtaattcctcgcttgccgattacgcaaagctgcgcccggcgtttgatcgcaaacacggaacggtaacggcggcaaacagtacgccgctgaccgatggcgcggcagcggtgatcctgatgactgaatcccgggcgaaagaattagggctggtgccgctggggtatctgcgcagctacgcatttactgcgattgatgtctggcaggacatgttgctcggtccagcctggtcaacaccgctggcgctggagcgtgccggtttgacgatgagcgatctgacattgatcgatatgcacgaagcctttgcagctcagacgctggcgaatattcagttgctgggtagtgaacgttttgctcgtgaagcactggggcgtgcacatgccactggcgaagtggacgatagcaaatttaacgtgcttggcggttcgattgcttacgggcatcccttcgcggcgaccggcgcgcggatgattacccagacattgcatgaacttcgccgtcgcggcggtggatttggtttagttaccgcctgtgctgccggtgggcttggcgcggcaatggttctggaggcggaataa BBa_K861044_sequence 1 attaaagaggagaaatactagatgggtcaggttttaccgctggttacccgccagggcgatcgtatcgccattgttagcggtttacgtacgccttttgcccgtcaggcgacggcttttcatggcattcccgcggttgatttagggaagatggtggtaggcgaactgctggcacgcagcgagatccccgccgaagtgattgaacaactggtctttggtcaggtcgtacaaatgcctgaagcccccaacattgcgcgtgaaattgttctcggtacgggaatgaatgtacataccgatgcttacagcgtcagccgcgcttgcgctaccagtttccaggcagttgcaaacgtcgcagaaagcctgatggcgggaactattcgagcggggattgccggtggggcagattcctcttcggtattgccaattggcgtcagtaaaaaactggcgcgcgtgctggttgatgtcaacaaagctcgtaccatgagccagcgactgaaactcttctctcgcctgcgtttgcgcgacttaatgcccgtaccacctgcggtagcagaatattctaccggcttgcggatgggcgacaccgcagagcaaatggcgaaaacctacggcatcacccgagaacagcaagatgcattagcgcaccgttcgcatcagcgtgccgctcaggcatggtcagacggaaaactcaaagaagaggtgatgactgcctttatccctccttataaacaaccgcttgtcgaagacaacaatattcgcggtaattcctcgcttgccgattacgcaaagctgcgcccggcgtttgatcgcaaacacggaacggtaacggcggcaaacagtacgccgctgaccgatggcgcggcagcggtgatcctgatgactgaatcccgggcgaaagaattagggctggtgccgctggggtatctgcgcagctacgcatttactgcgattgatgtctggcaggacatgttgctcggtccagcctggtcaacaccgctggcgctggagcgtgccggtttgacgatgagcgatctgacattgatcgatatgcacgaagcctttgcagctcagacgctggcgaatattcagttgctgggtagtgaacgttttgctcgtgaagcactggggcgtgcacatgccactggcgaagtggacgatagcaaatttaacgtgcttggcggttcgattgcttacgggcatcccttcgcggcgaccggcgcgcggatgattacccagacattgcatgaacttcgccgtcgcggcggtggatttggtttagttaccgcctgtgctgccggtgggcttggcgcggcaatggttctggaggcggaataa BBa_B0030_sequence 1 attaaagaggagaaa BBa_R0011_sequence 1 aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z