LOCUS       BBa_K863005             1605 bp    DNA     linear       08-May-2024
DEFINITION  ecol laccase from E. coli with T7 promoter, RBS and His-tag
ACCESSION   BBa_K863005
VERSION     BBa_K863005.1  
FEATURES             Location/Qualifiers
     promoter        1..23
                     /label=T7 promoter
     misc_feature    34..36
                     /label=ATG
     misc_feature    1603..1605
                     /label=TAA
     misc_feature    1600..1602
                     /label=TAA
     misc_feature    1582..1599
                     /label=6x HIS 
     CDS             34..1581
                     /label=ecol
     RBS             25..30
                     /label=RBS
ORIGIN
        1 taatacgact cactataggg agagaggaga aaaatgcaac gtcgtgattt cttaaaatat
       61 tccgtcgcgc tgggtgtggc ttcggctttg ccgctgtgga accgcgcagt atttgcggca
      121 gaacgcccaa cgttaccgat ccctgatttg ctcacgaccg atgcccgtaa tcgcattcag
      181 ttaactattg gcgcaggcca gtccaccttt ggcgggaaaa ctgcaactac ctggggctat
      241 aacggcaatc tgctgggacc ggcggtgaaa ttacagcgcg gcaaagcggt aacggttgat
      301 atctacaacc aactgacgga agagacaacg ttgcactggc acgggctgga agtaccgggt
      361 gaagtcgacg gcggcccgca gggaattatt ccgccaggtg gcaagcgctc ggtgacgttg
      421 aacgttgatc aacctgccgc tacctgctgg ttccatccgc atcagcacgg caaaaccggg
      481 cgacaggtgg cgatggggct ggctgggctg gtggtgattg aagatgacga gatcctgaaa
      541 ttaatgctgc caaaacagtg gggtatcgat gatgttccgg tgatcgttca ggataagaaa
      601 tttagcgccg acgggcagat tgattatcaa ctggatgtga tgaccgccgc cgtgggctgg
      661 tttggcgata cgttgctgac caacggtgca atctacccgc aacacgctgc cccgcgtggt
      721 tggctgcgcc tgcgtttgct caatggctgt aatgcccgtt cgctcaattt cgccaccagc
      781 gacaatcgcc cgctgtatgt gattgccagc gacggtggtc tgctacctga accagtgaag
      841 gtgagcgaac tgccggtgct gatgggcgag cgttttgaag tgctggtgga ggttaacgat
      901 aacaaaccct ttgacctggt gacgctgccg gtcagccaga tggggatggc gattgcgccg
      961 tttgataagc ctcatccggt aatgcggatt cagccgattg ctattagtgc ctccggtgct
     1021 ttgccagaca cattaagtag cctgcctgcg ttaccttcgc tggaagggct gacggtacgc
     1081 aagctgcaac tctctatgga cccgatgctc gatatgatgg ggatgcagat gctaatggag
     1141 aaatatggcg atcaggcgat ggccgggatg gatcacagcc agatgatggg ccatatgggg
     1201 cacggcaata tgaatcatat gaaccacggc gggaagttcg atttccacca tgccaacaaa
     1261 atcaacggtc aggcgtttga tatgaacaag ccgatgtttg cggcggcgaa agggcaatac
     1321 gaacgttggg ttatctctgg cgtgggcgac atgatgctgc atccgttcca tatccacggc
     1381 acgcagttcc gtatcttgtc agaaaatggc aaaccgccag cggctcatcg cgcgggctgg
     1441 aaagataccg ttaaggtaga aggtaatgtc agcgaagtgc tggtgaagtt taatcacgat
     1501 gcaccgaaag aacatgctta tatggcgcac tgccatctgc tggagcatga agatacgggg
     1561 atgatgttag ggtttacggt acatcaccat caccatcact aataa
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