LOCUS       BBa_K864120             1751 bp    DNA     linear       10-May-2024
DEFINITION  pMB1 ori - RFP cassette
ACCESSION   BBa_K864120
VERSION     BBa_K864120.1  
FEATURES             Location/Qualifiers
     misc_feature    16..630
                     /label=rep (pMB1)
     promoter        27..32
                     /label=RNAII transcript promoter -35 sequence
     misc_feature    27..615
                     /label=origin of replication
     promoter        48..53
                     /label=RNAII transcript promoter -10 sequence
     misc_feature    63..615
                     /label=RNAII transcript
     misc_feature    66..173
                     /label=RNAI transcript
     promoter        182..187
                     /label=RNAI transcript promoter -10
     promoter        204..209
                     /label=RNAI transcript promoter -35
     misc_feature    615..615
                     /label=ORI
     misc_feature    1..674
                     /label=BBa_I50022
     CDS             683..770
                     /label=end of LacI coding region (inactive)
     misc_feature    683..882
                     /label=BBa_R0010
     misc_feature    771..808
                     /label=CAP binding site
     promoter        819..824
                     /label=-35
     promoter        843..848
                     /label=-10
     misc_feature    848..882
                     /label=LacI binding site
     misc_feature    855..855
                     /label=start
     promoter        683..882
                     /label=BBa_R0010
     misc_feature    895..898
                     /label=conserved
     RBS             891..902
                     /label=BBa_B0034
     CDS             909..1583
                     /label=mrfp1
     misc_feature    1590..1614
                     /label=Help:Barcodes
     CDS             909..1589
                     /label=BBa_E1010
     stem_loop       1634..1677
                     /label=stem_loop
     misc_feature    1623..1702
                     /label=BBa_B0010
     terminator      1623..1702
                     /label=BBa_B0010
     stem_loop       1718..1737
                     /label=T7 TE
     misc_feature    1744..1744
                     /label=stop
     polyA_site      1738..1751
                     /label=polya
     misc_feature    1711..1751
                     /label=BBa_B0012
     terminator      1711..1751
                     /label=BBa_B0012
     misc_feature    683..1751
                     /label=BBa_J04450
ORIGIN
        1 cccgtagaaa agatcaaaag atcttcttga gatccttttt ttctgcgcgt aatctgctac
       61 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
      121 actctttttc cgaaggtaac tggctttagc agagcgcaga taccaaatac tgtccttcta
      181 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
      241 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
      301 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
      361 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
      421 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
      481 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
      541 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
      601 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
      661 ccttttgctc acattactag agcaatacgc aaaccgcctc tccccgcgcg ttggccgatt
      721 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
      781 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
      841 cgtatgttgt gtggaattgt gagcggataa caatttcaca catactagag aaagaggaga
      901 aatactagat ggcttcctcc gaagacgtta tcaaagagtt catgcgtttc aaagttcgta
      961 tggaaggttc cgttaacggt cacgagttcg aaatcgaagg tgaaggtgaa ggtcgtccgt
     1021 acgaaggtac ccagaccgct aaactgaaag ttaccaaagg tggtccgctg ccgttcgctt
     1081 gggacatcct gtccccgcag ttccagtacg gttccaaagc ttacgttaaa cacccggctg
     1141 acatcccgga ctacctgaaa ctgtccttcc cggaaggttt caaatgggaa cgtgttatga
     1201 acttcgaaga cggtggtgtt gttaccgtta cccaggactc ctccctgcaa gacggtgagt
     1261 tcatctacaa agttaaactg cgtggtacca acttcccgtc cgacggtccg gttatgcaga
     1321 aaaaaaccat gggttgggaa gcttccaccg aacgtatgta cccggaagac ggtgctctga
     1381 aaggtgaaat caaaatgcgt ctgaaactga aagacggtgg tcactacgac gctgaagtta
     1441 aaaccaccta catggctaaa aaaccggttc agctgccggg tgcttacaaa accgacatca
     1501 aactggacat cacctcccac aacgaagact acaccatcgt tgaacagtac gaacgtgctg
     1561 aaggtcgtca ctccaccggt gcttaataac gctgatagtg ctagtgtaga tcgctactag
     1621 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg
     1681 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt
     1741 ctgcgtttat a
//