LOCUS       BBa_K876000              891 bp    DNA     linear       12-May-2024
DEFINITION  pLac-TetR
ACCESSION   BBa_K876000
VERSION     BBa_K876000.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     CDS             210..826
                     /label=tetR
     misc_feature    827..860
                     /label=SsrA
     misc_feature    867..891
                     /label=Help:Barcodes
     CDS             207..891
                     /label=BBa_C0040
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagatgt ccagattaga taaaagtaaa gtgattaaca
      241 gcgcattaga gctgcttaat gaggtcggaa tcgaaggttt aacaacccgt aaactcgccc
      301 agaagctagg tgtagagcag cctacattgt attggcatgt aaaaaataag cgggctttgc
      361 tcgacgcctt agccattgag atgttagata ggcaccatac tcacttttgc cctttagaag
      421 gggaaagctg gcaagatttt ttacgtaata acgctaaaag ttttagatgt gctttactaa
      481 gtcatcgcga tggagcaaaa gtacatttag gtacacggcc tacagaaaaa cagtatgaaa
      541 ctctcgaaaa tcaattagcc tttttatgcc aacaaggttt ttcactagag aatgcattat
      601 atgcactcag cgctgtgggg cattttactt taggttgcgt attggaagat caagagcatc
      661 aagtcgctaa agaagaaagg gaaacaccta ctactgatag tatgccgcca ttattacgac
      721 aagctatcga attatttgat caccaaggtg cagagccagc cttcttattc ggccttgaat
      781 tgatcatatg cggattagaa aaacaactta aatgtgaaag tgggtccgct gcaaacgacg
      841 aaaactacgc tttagtagct taataacact gatagtgcta gtgtagatca c
//