LOCUS       BBa_K876004             1749 bp    DNA     linear       12-May-2024
DEFINITION  pTet-RBS-GFP-T-pLac-TetR
ACCESSION   BBa_K876004
VERSION     BBa_K876004.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     RBS             63..77
                     /label=RBS-1\Strong
     RBS             70..74
                     /label=RBS
     misc_feature    63..77
                     /label=BBa_B0030
     RBS             63..77
                     /label=BBa_B0030
     CDS             84..803
                     /label=GFP protein
     CDS             84..803
                     /label=BBa_E0040
     misc_feature    812..850
                     /label=B1006
     stem_loop       821..842
                     /label=modified thr terminator
     polyA_site      843..850
                     /label=PolyA
     polyA_site      812..820
                     /label=PolyA
     terminator      812..850
                     /label=BBa_B1006
     misc_feature    63..850
                     /label=BBa_K081012
     CDS             859..946
                     /label=end of LacI coding region (inactive)
     misc_feature    859..1058
                     /label=BBa_R0010
     misc_feature    947..984
                     /label=CAP binding site
     promoter        995..1000
                     /label=-35
     promoter        1019..1024
                     /label=-10
     misc_feature    1024..1058
                     /label=LacI binding site
     misc_feature    1031..1031
                     /label=start
     promoter        859..1058
                     /label=BBa_R0010
     CDS             1068..1684
                     /label=tetR
     misc_feature    1685..1718
                     /label=SsrA
     misc_feature    1725..1749
                     /label=Help:Barcodes
     CDS             1065..1749
                     /label=BBa_C0040
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agattaaaga ggagaaatac tagatgcgta aaggagaaga acttttcact ggagttgtcc
      121 caattcttgt tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg
      181 gtgaaggtga tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac
      241 tacctgttcc atggccaaca cttgtcacta ctttcggtta tggtgttcaa tgctttgcga
      301 gatacccaga tcatatgaaa cagcatgact ttttcaagag tgccatgccc gaaggttatg
      361 tacaggaaag aactatattt ttcaaagatg acgggaacta caagacacgt gctgaagtca
      421 agtttgaagg tgataccctt gttaatagaa tcgagttaaa aggtattgat tttaaagaag
      481 atggaaacat tcttggacac aaattggaat acaactataa ctcacacaat gtatacatca
      541 tggcagacaa acaaaagaat ggaatcaaag ttaacttcaa aattagacac aacattgaag
      601 atggaagcgt tcaactagca gaccattatc aacaaaatac tccaattggc gatggccctg
      661 tccttttacc agacaaccat tacctgtcca cacaatctgc cctttcgaaa gatcccaacg
      721 aaaagagaga ccacatggtc cttcttgagt ttgtaacagc tgctgggatt acacatggca
      781 tggatgaact atacaaataa taatactaga gaaaaaaaaa ccccgcccct gacagggcgg
      841 ggtttttttt tactagagca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
      901 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
      961 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     1021 tgttgtgtgg aattgtgagc ggataacaat ttcacacata ctagatgtcc agattagata
     1081 aaagtaaagt gattaacagc gcattagagc tgcttaatga ggtcggaatc gaaggtttaa
     1141 caacccgtaa actcgcccag aagctaggtg tagagcagcc tacattgtat tggcatgtaa
     1201 aaaataagcg ggctttgctc gacgccttag ccattgagat gttagatagg caccatactc
     1261 acttttgccc tttagaaggg gaaagctggc aagatttttt acgtaataac gctaaaagtt
     1321 ttagatgtgc tttactaagt catcgcgatg gagcaaaagt acatttaggt acacggccta
     1381 cagaaaaaca gtatgaaact ctcgaaaatc aattagcctt tttatgccaa caaggttttt
     1441 cactagagaa tgcattatat gcactcagcg ctgtggggca ttttacttta ggttgcgtat
     1501 tggaagatca agagcatcaa gtcgctaaag aagaaaggga aacacctact actgatagta
     1561 tgccgccatt attacgacaa gctatcgaat tatttgatca ccaaggtgca gagccagcct
     1621 tcttattcgg ccttgaattg atcatatgcg gattagaaaa acaacttaaa tgtgaaagtg
     1681 ggtccgctgc aaacgacgaa aactacgctt tagtagctta ataacactga tagtgctagt
     1741 gtagatcac
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