LOCUS       BBa_K876049             1605 bp    DNA     linear       14-May-2024
DEFINITION  plac-lasI-RBS-RFP
ACCESSION   BBa_K876049
VERSION     BBa_K876049.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     CDS             207..809
                     /label=LasI
     misc_feature    810..842
                     /label=LVA
     misc_feature    849..873
                     /label=Help:Barcodes
     CDS             207..873
                     /label=BBa_C0078
     misc_feature    886..889
                     /label=conserved
     RBS             882..893
                     /label=BBa_B0034
     CDS             900..1574
                     /label=mrfp1
     misc_feature    1581..1605
                     /label=Help:Barcodes
     CDS             900..1605
                     /label=BBa_E1010
     misc_feature    882..1605
                     /label=BBa_K093005
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagatga tcgttcagat cggtcgtcgt gaagagttcg
      241 acaaaaaact gctgggtgaa atgcacaaac tgcgtgctca ggttttcaaa gaacgtaaag
      301 gttgggacgt ttccgttatc gacgaaatgg aaatcgacgg ttacgacgct ctgtccccgt
      361 actacatgct gatccaggaa gacaccccgg aagctcaggt tttcggttgc tggcgtatct
      421 tcgacaccac cggtccgtac atgctgaaaa acaccttccc ggaactgctg cacggtaaag
      481 aagctccgtg ctccccgcac atctgggaac tgtcccgttt cgctatcaac tccggtcaga
      541 aaggttccct gggtttctcc gactgcaccc tggaagctat gcgtgctctg gctcgttact
      601 ccttgcagaa cgacatccag accctggtta ccgttaccac cgttggtgtt gaaaaaatga
      661 tgatccgtgc tggtctggac gtttcccgtt tcggtccgca cctgaaaatc ggtatcgaac
      721 gtgctgttgc tctgcgtatc gaactgaacg ctaaaaccca gatcgctctg tacggtggtg
      781 ttctggttga acagcgtctg gctgtttccg ctgctaacga cgaaaactac gctctggttg
      841 cttaataact ctgatagtgc tagtgtagat ctctactaga gaaagaggag aaatactaga
      901 tggcttcctc cgaagacgtt atcaaagagt tcatgcgttt caaagttcgt atggaaggtt
      961 ccgttaacgg tcacgagttc gaaatcgaag gtgaaggtga aggtcgtccg tacgaaggta
     1021 cccagaccgc taaactgaaa gttaccaaag gtggtccgct gccgttcgct tgggacatcc
     1081 tgtccccgca gttccagtac ggttccaaag cttacgttaa acacccggct gacatcccgg
     1141 actacctgaa actgtccttc ccggaaggtt tcaaatggga acgtgttatg aacttcgaag
     1201 acggtggtgt tgttaccgtt acccaggact cctccctgca agacggtgag ttcatctaca
     1261 aagttaaact gcgtggtacc aacttcccgt ccgacggtcc ggttatgcag aaaaaaacca
     1321 tgggttggga agcttccacc gaacgtatgt acccggaaga cggtgctctg aaaggtgaaa
     1381 tcaaaatgcg tctgaaactg aaagacggtg gtcactacga cgctgaagtt aaaaccacct
     1441 acatggctaa aaaaccggtt cagctgccgg gtgcttacaa aaccgacatc aaactggaca
     1501 tcacctccca caacgaagac tacaccatcg ttgaacagta cgaacgtgct gaaggtcgtc
     1561 actccaccgg tgcttaataa cgctgatagt gctagtgtag atcgc
//