LOCUS       BBa_K876061             1597 bp    DNA     linear       14-May-2024
DEFINITION  Term-Term-pluxR-LasI-RBS-RFP
ACCESSION   BBa_K876061
VERSION     BBa_K876061.1  
FEATURES             Location/Qualifiers
     stem_loop       12..55
                     /label=stem_loop
     misc_feature    1..80
                     /label=BBa_B0010
     terminator      1..80
                     /label=BBa_B0010
     stem_loop       96..115
                     /label=T7 TE
     misc_feature    122..122
                     /label=stop
     polyA_site      116..129
                     /label=polya
     misc_feature    89..129
                     /label=BBa_B0012
     terminator      89..129
                     /label=BBa_B0012
     terminator      1..129
                     /label=BBa_B0015
     misc_feature    138..157
                     /label=LuxR/HSL
     promoter        157..162
                     /label=-35
     promoter        179..184
                     /label=-10
     misc_feature    190..190
                     /label=start
     misc_feature    138..192
                     /label=BBa_R0062
     promoter        138..192
                     /label=BBa_R0062
     CDS             199..801
                     /label=LasI
     misc_feature    802..834
                     /label=LVA
     misc_feature    841..865
                     /label=Help:Barcodes
     CDS             199..865
                     /label=BBa_C0078
     misc_feature    878..881
                     /label=conserved
     RBS             874..885
                     /label=BBa_B0034
     CDS             892..1566
                     /label=mrfp1
     misc_feature    1573..1597
                     /label=Help:Barcodes
     CDS             892..1597
                     /label=BBa_E1010
     misc_feature    874..1597
                     /label=BBa_K093005
ORIGIN
        1 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
       61 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
      121 gcgtttatat actagagacc tgtaggatcg tacaggttta cgcaagaaaa tggtttgtta
      181 tagtcgaata aatactagat gatcgttcag atcggtcgtc gtgaagagtt cgacaaaaaa
      241 ctgctgggtg aaatgcacaa actgcgtgct caggttttca aagaacgtaa aggttgggac
      301 gtttccgtta tcgacgaaat ggaaatcgac ggttacgacg ctctgtcccc gtactacatg
      361 ctgatccagg aagacacccc ggaagctcag gttttcggtt gctggcgtat cttcgacacc
      421 accggtccgt acatgctgaa aaacaccttc ccggaactgc tgcacggtaa agaagctccg
      481 tgctccccgc acatctggga actgtcccgt ttcgctatca actccggtca gaaaggttcc
      541 ctgggtttct ccgactgcac cctggaagct atgcgtgctc tggctcgtta ctccttgcag
      601 aacgacatcc agaccctggt taccgttacc accgttggtg ttgaaaaaat gatgatccgt
      661 gctggtctgg acgtttcccg tttcggtccg cacctgaaaa tcggtatcga acgtgctgtt
      721 gctctgcgta tcgaactgaa cgctaaaacc cagatcgctc tgtacggtgg tgttctggtt
      781 gaacagcgtc tggctgtttc cgctgctaac gacgaaaact acgctctggt tgcttaataa
      841 ctctgatagt gctagtgtag atctctacta gagaaagagg agaaatacta gatggcttcc
      901 tccgaagacg ttatcaaaga gttcatgcgt ttcaaagttc gtatggaagg ttccgttaac
      961 ggtcacgagt tcgaaatcga aggtgaaggt gaaggtcgtc cgtacgaagg tacccagacc
     1021 gctaaactga aagttaccaa aggtggtccg ctgccgttcg cttgggacat cctgtccccg
     1081 cagttccagt acggttccaa agcttacgtt aaacacccgg ctgacatccc ggactacctg
     1141 aaactgtcct tcccggaagg tttcaaatgg gaacgtgtta tgaacttcga agacggtggt
     1201 gttgttaccg ttacccagga ctcctccctg caagacggtg agttcatcta caaagttaaa
     1261 ctgcgtggta ccaacttccc gtccgacggt ccggttatgc agaaaaaaac catgggttgg
     1321 gaagcttcca ccgaacgtat gtacccggaa gacggtgctc tgaaaggtga aatcaaaatg
     1381 cgtctgaaac tgaaagacgg tggtcactac gacgctgaag ttaaaaccac ctacatggct
     1441 aaaaaaccgg ttcagctgcc gggtgcttac aaaaccgaca tcaaactgga catcacctcc
     1501 cacaacgaag actacaccat cgttgaacag tacgaacgtg ctgaaggtcg tcactccacc
     1561 ggtgcttaat aacgctgata gtgctagtgt agatcgc
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