LOCUS       BBa_K876065             2717 bp    DNA     linear       18-May-2024
DEFINITION  pTetR-lasR-TT-plasR-luxI-RBS-YFP-Term-Term
ACCESSION   BBa_K876065
VERSION     BBa_K876065.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    61..62
                     /label=prefix
     misc_feature    234..234
                     /label=T
     misc_feature    552..552
                     /label=A
     CDS             61..810
                     /label=luxR
     misc_feature    61..816
                     /label=BBa_C0062
     misc_feature    817..841
                     /label=Help:Barcodes
     CDS             61..816
                     /label=BBa_C0062
     stem_loop       861..904
                     /label=stem_loop
     misc_feature    850..929
                     /label=BBa_B0010
     terminator      850..929
                     /label=BBa_B0010
     stem_loop       945..964
                     /label=T7 TE
     misc_feature    971..971
                     /label=stop
     polyA_site      965..978
                     /label=polya
     misc_feature    938..978
                     /label=BBa_B0012
     terminator      938..978
                     /label=BBa_B0012
     terminator      850..978
                     /label=BBa_B0015
     misc_feature    1032..1049
                     /label=OP2
     misc_feature    1092..1111
                     /label=OP1
     promoter        1103..1108
                     /label=-35
     promoter        1126..1131
                     /label=-10
     promoter        987..1143
                     /label=BBa_R0079
     misc_feature    1729..1760
                     /label=LVA
     CDS             1150..1728
                     /label=luxI
     misc_feature    1150..1767
                     /label=BBa_C0061
     misc_feature    1768..1792
                     /label=Help:Barcodes
     CDS             1150..1767
                     /label=BBa_C0061
     misc_feature    1805..1808
                     /label=conserved
     RBS             1801..1812
                     /label=BBa_B0034
     CDS             1819..2580
                     /label=YFP (LVA)
     misc_feature    2575..2580
                     /label=2
     misc_feature    2536..2574
                     /label=SsrA
     misc_feature    2060..2060
                     /label=A (Q->L)
     misc_feature    1819..2580
                     /label=BBa_E0032
     misc_feature    1819..2580
                     /label=BBa_E0032
     stem_loop       2600..2643
                     /label=stem_loop
     misc_feature    2589..2668
                     /label=BBa_B0010
     terminator      2589..2668
                     /label=BBa_B0010
     stem_loop       2684..2703
                     /label=T7 TE
     misc_feature    2710..2710
                     /label=stop
     polyA_site      2704..2717
                     /label=polya
     misc_feature    2677..2717
                     /label=BBa_B0012
     terminator      2677..2717
                     /label=BBa_B0012
     misc_feature    1801..2717
                     /label=BBa_E0432
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 atgaaaaaca taaatgccga cgacacatac agaataatta ataaaattaa agcttgtaga
      121 agcaataatg atattaatca atgcttatct gatatgacta aaatggtaca ttgtgaatat
      181 tatttactcg cgatcattta tcctcattct atggttaaat ctgatatttc aatcctagat
      241 aattacccta aaaaatggag gcaatattat gatgacgcta atttaataaa atatgatcct
      301 atagtagatt attctaactc caatcattca ccaattaatt ggaatatatt tgaaaacaat
      361 gctgtaaata aaaaatctcc aaatgtaatt aaagaagcga aaacatcagg tcttatcact
      421 gggtttagtt tccctattca tacggctaac aatggcttcg gaatgcttag ttttgcacat
      481 tcagaaaaag acaactatat agatagttta tttttacatg cgtgtatgaa cataccatta
      541 attgttcctt ctctagttga taattatcga aaaataaata tagcaaataa taaatcaaac
      601 aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg catgcgaagg aaaaagctct
      661 tgggatattt caaaaatatt aggttgcagt gagcgtactg tcactttcca tttaaccaat
      721 gcgcaaatga aactcaatac aacaaaccgc tgccaaagta tttctaaagc aattttaaca
      781 ggagcaattg attgcccata ctttaaaaat taataacact gatagtgcta gtgtagatca
      841 ctactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
      901 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg
      961 ggcctttctg cgtttatata ctagaggccc ctcgctgagc gcgtcccgga gctgggggca
     1021 acctagctgc cacctgcttt tctgctagct attccagcga aaacatacag atttccggcg
     1081 aaatcaaggc tacctgccag ttctggcagg tttggccgcg ggttcttttt ggtacacgaa
     1141 agctactaga tgactataat gataaaaaaa tcggattttt tggcaattcc atcggaggag
     1201 tataaaggta ttctaagtct tcgttatcaa gtgtttaagc aaagacttga gtgggactta
     1261 gttgtagaaa ataaccttga atcagatgag tatgataact caaatgcaga atatatttat
     1321 gcttgtgatg atactgaaaa tgtaagtgga tgctggcgtt tattacctac aacaggtgat
     1381 tatatgctga aaagtgtttt tcctgaattg cttggtcaac agagtgctcc caaagatcct
     1441 aatatagtcg aattaagtcg ttttgctgta ggtaaaaata gctcaaagat aaataactct
     1501 gctagtgaaa ttacaatgaa actatttgaa gctatatata aacacgctgt tagtcaaggt
     1561 attacagaat atgtaacagt aacatcaaca gcaatagagc gatttttaaa gcgtattaaa
     1621 gttccttgtc atcgtattgg agacaaagaa attcatgtat taggtgatac taaatcggtt
     1681 gtattgtcta tgcctattaa tgaacagttt aaaaaagcag tcttaaatgc tgcaaacgac
     1741 gaaaactacg ctttagtagc ttaataactc tgatagtgct agtgtagatc tctactagag
     1801 aaagaggaga aatactagat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
     1861 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
     1921 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
     1981 cccgtgccct ggcccaccct cgtgaccacc ttcggctacg gcctgcaatg cttcgcccgc
     2041 taccccgacc acatgaagct gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
     2101 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
     2161 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
     2221 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg
     2281 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac
     2341 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
     2401 ctgctgcccg acaaccacta cctgagctac cagtccgccc tgagcaaaga ccccaacgag
     2461 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
     2521 gacgagctgt acaagaggcc tgctgcaaac gacgaaaact acgctttagt agcttaataa
     2581 tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
     2641 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg
     2701 gcctttctgc gtttata
//