LOCUS       BBa_K880002             1219 bp    DNA     linear       13-May-2024
DEFINITION  GFP fluorescence reporter to assay the activity of FimE K137007 
            and HbiF K880000 recombinases.
ACCESSION   BBa_K880002
VERSION     BBa_K880002.1  
FEATURES             Location/Qualifiers
     misc_feature    1..17
                     /label=IRR out
     misc_feature    19..35
                     /label=IRR in
     misc_feature    1..35
                     /label=BBa_K137008
     promoter        44..97
                     /label=tetR promoter
     promoter        44..293
                     /label=BBa_K137047
     misc_feature    302..318
                     /label=IRL in
     misc_feature    320..336
                     /label=IRL out
     misc_feature    302..336
                     /label=BBa_K137010
     misc_feature    349..352
                     /label=conserved
     RBS             345..356
                     /label=BBa_B0034
     CDS             363..1082
                     /label=GFP protein
     CDS             363..1082
                     /label=BBa_E0040
     stem_loop       1102..1145
                     /label=stem_loop
     misc_feature    1091..1170
                     /label=BBa_B0010
     terminator      1091..1170
                     /label=BBa_B0010
     stem_loop       1186..1205
                     /label=T7 TE
     misc_feature    1212..1212
                     /label=stop
     polyA_site      1206..1219
                     /label=polya
     misc_feature    1179..1219
                     /label=BBa_B0012
     terminator      1179..1219
                     /label=BBa_B0012
     misc_feature    345..1219
                     /label=BBa_I13504
ORIGIN
        1 aagatgaaac atttggggcc aaactgtcca tattatacta gaggtgctca gtatctctat
       61 cactgatagg gatgtcaatc tctatcactg atagggactc tagtatataa acgcagaaag
      121 gcccacccga aggtgagcca gtgtgactct agtagagagc gttcaccgac aaacaacaga
      181 taaaacgaaa ggcccagtct ttcgactgag cctttcgttt tatttgatgc ctggctctag
      241 tagtgatcta cactagcact atcagtgtta ttaagctact aaagcgtagt ttttactaga
      301 gtctatgagt caaaatggcc ccaattgtct tgtatttact agagaaagag gagaaatact
      361 agatgcgtaa aggagaagaa cttttcactg gagttgtccc aattcttgtt gaattagatg
      421 gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat gcaacatacg
      481 gaaaacttac ccttaaattt atttgcacta ctggaaaact acctgttcca tggccaacac
      541 ttgtcactac tttcggttat ggtgttcaat gctttgcgag atacccagat catatgaaac
      601 agcatgactt tttcaagagt gccatgcccg aaggttatgt acaggaaaga actatatttt
      661 tcaaagatga cgggaactac aagacacgtg ctgaagtcaa gtttgaaggt gatacccttg
      721 ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt cttggacaca
      781 aattggaata caactataac tcacacaatg tatacatcat ggcagacaaa caaaagaatg
      841 gaatcaaagt taacttcaaa attagacaca acattgaaga tggaagcgtt caactagcag
      901 accattatca acaaaatact ccaattggcg atggccctgt ccttttacca gacaaccatt
      961 acctgtccac acaatctgcc ctttcgaaag atcccaacga aaagagagac cacatggtcc
     1021 ttcttgagtt tgtaacagct gctgggatta cacatggcat ggatgaacta tacaaataat
     1081 aatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
     1141 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt
     1201 gggcctttct gcgtttata
//