LOCUS BBa_K887004 8924 bp DNA linear 19-May-2024 DEFINITION Plac+alsS+ilvC+ilvD(each preceded by own zinc-finger and RBS)+Ptet+B0032+kivD+B0015 ACCESSION BBa_K887004 VERSION BBa_K887004.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 misc_feature 213..216 /label=conserved RBS 209..220 /label=BBa_B0034 misc_feature 229..522 /label=BBa_K323105 CDS 529..2244 /label=AlsS CDS 529..2244 /label=BBa_K539713 misc_feature 2257..2260 /label=conserved RBS 2253..2264 /label=BBa_B0034 misc_feature 2273..2560 /label=BBa_K323107 CDS 2567..4042 /label=ilvC coding sequence CDS 2567..4042 /label=BBa_K539711 misc_feature 4055..4058 /label=conserved RBS 4051..4062 /label=BBa_B0034 misc_feature 4071..4322 /label=BBa_K165006 CDS 4329..6179 /label=ilvD CDS 4329..6179 /label=BBa_K539712 RBS 6234..6239 /label=rbs misc_feature 6210..6215 /label=scar adaptation stem_loop 6188..6205 /label=stem_loop stem_loop 6211..6239 /label=predicted stem-loop extending to one position before start codon misc_feature 6188..6239 /label=BBa_K115002 CDS 6249..6865 /label=tetR misc_feature 6866..6899 /label=SsrA misc_feature 6906..6930 /label=Help:Barcodes CDS 6246..6930 /label=BBa_C0040 terminator 6939..7051 /label=BBa_J61048 misc_feature 7060..7078 /label=TetR 1 misc_feature 7060..7113 /label=BBa_R0040 promoter 7079..7084 /label=-35 misc_feature 7085..7103 /label=TetR 2 promoter 7102..7107 /label=-10 promoter 7060..7113 /label=BBa_R0040 RBS 7122..7134 /label=RBS-3\Weak RBS 7128..7131 /label=RBS misc_feature 7122..7134 /label=BBa_B0032 RBS 7122..7134 /label=BBa_B0032 CDS 7141..8787 /label=kivd coding sequence CDS 7141..8787 /label=BBa_K539714 stem_loop 8807..8850 /label=stem_loop misc_feature 8796..8875 /label=BBa_B0010 terminator 8796..8875 /label=BBa_B0010 stem_loop 8891..8910 /label=T7 TE misc_feature 8917..8917 /label=stop polyA_site 8911..8924 /label=polya misc_feature 8884..8924 /label=BBa_B0012 terminator 8884..8924 /label=BBa_B0012 terminator 8796..8924 /label=BBa_B0015 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagagcc gggtgaaaaa 241 ccgtatgcat gtccggttga aagctgtgat cgtcgtttta gccgtagtga tgaactgacc 301 cgtcatattc gtattcatac aggtcagaaa ccgtttcagt gtcgtatttg catgcgtaat 361 tttagccgtt cagatcatct gaccacccat attcgtaccc atactggcga aaaaccgttt 421 gcctgtgata tttgtggtcg taaatttgca cgttccgatg aacgtaaacg ccataccaaa 481 attcatacgg gtggtggtag cggtggtggt tcaggtggta gttactagat gttgacaaaa 541 gcaacaaaag aacaaaaatc ccttgtgaaa aacagagggg cggagcttgt tgttgattgc 601 ttagtggagc aaggtgtcac acatgtattt ggcattccag gtgcaaaaat tgatgcggta 661 tttgacgctt tacaagataa aggacctgaa attatcgttg cccggcacga acaaaacgca 721 gcattcatgg cccaagcagt cggccgttta actggaaaac cgggagtcgt gttagtcaca 781 tcaggaccgg gtgcctctaa cttggcaaca ggcctgctga cagcgaacac tgaaggagac 841 cctgtcgttg cgcttgctgg aaacgtgatc cgtgcagatc gtttaaaacg gacacatcaa 901 tctttggata atgcggcgct attccagccg attacaaaat acagtgtaga agttcaagat 961 gtaaaaaata taccggaagc tgttacaaat gcatttagga tagcgtcagc agggcaggct 1021 ggggccgctt ttgtgagctt tccgcaagat gttgtgaatg aagtcacaaa tacgaaaaac 1081 gtgcgtgctg ttgcagcgcc aaaactcggt ccagcagcag atgatgcaat cagtgcggcc 1141 atagcaaaaa tccaaacagc aaaacttcct gtcgttttgg tcggcatgaa aggcggaaga 1201 ccggaagcaa ttaaggcggt tcgcaagctt ttgaaaaagg ttcagcttcc atttgttgaa 1261 acatatcaag ctgccggtac cctttccaga gatttagagg atcaatattt tggccgtatc 1321 ggtttgttcc gcaaccagcc tggcgattta ctgctagagc aggcagatgt tgttctgacg 1381 atcggctatg acccgattga atatgatccg aaattctgga atatcaatgg agaccggaca 1441 attatccatt tagacgagat tatcgctgac attgatcatg cttaccagcc tgatcttgaa 1501 ttgatcggtg acattccgtc cacgatcaat catatcgaac acgatgctgt gaaagtggaa 1561 tttgcagagc gtgagcagaa aatcctttct gatttaaaac aatatatgca tgaaggtgag 1621 caggtgccag cagattggaa atcagacaga gcgcaccctc ttgaaatcgt taaagagttg 1681 cgtaatgcag tcgatgatca tgttacagta acttgcgata tcggttcgca cgccatttgg 1741 atgtcacgtt atttccgcag ctacgagccg ttaacattaa tgatcagtaa cggtatgcaa 1801 acactcggcg ttgcgcttcc ttgggcaatc ggcgcttcat tggtgaaacc gggagaaaaa 1861 gtggtttctg tctctggtga cggcggtttc ttattctcag caatggaatt agagacagca 1921 gttcgactaa aagcaccaat tgtacacatt gtatggaacg acagcacata tgacatggtt 1981 gcattccagc aattgaaaaa atataaccgt acatctgcgg tcgatttcgg aaatatcgat 2041 atcgtgaaat atgcggaaag cttcggagca actggcttgc gcgtagaatc accagaccag 2101 ctggcagatg ttctgcgtca aggcatgaac gctgaaggtc ctgtcatcat cgatgtcccg 2161 gttgactaca gtgataacat taatttagca agtgacaagc ttccgaaaga atttggggaa 2221 ctcatgaaaa cgaaagctct ctagtactag agaaagagga gaaatactag agccgggtga 2281 aaaaccgtat gcatgtcctg aatgtggtaa aagctttagc cagcgtgcaa atctgcgtgc 2341 acatcagcgt acccatacag gtgaaaagcc ttataaatgc ccagaatgcg gcaaaagctt 2401 ttcacgtagc gatcatctga ccacccatca gcgcacacat actggcgaga aaccttacaa 2461 atgtccagag tgtggtaaat catttagccg tagtgatgtt ctggttcgtc atcagagaac 2521 ccacacgggt ggtggtagcg gtggtggttc aggtggtagt tactagatgg ctaactactt 2581 caatacactg aatctgcgcc agcagctggc acagctgggc aaatgtcgct ttatgggccg 2641 cgatgaattt gccgatggcg cgagctacct tcagggtaaa aaagtagtca tcgtcggctg 2701 tggcgcacag ggtctgaacc agggcctgaa catgcgtgat tctggtctcg atatctccta 2761 cgctctgcgt aaagaagcga ttgccgagaa gcgcgcgtcc tggcgtaaag cgaccgaaaa 2821 tggttttaaa gtgggtactt acgaagaact gatcccacag gcggatctgg tgattaacct 2881 gacgccggac aagcagcact ctgatgtagt gcgcaccgta cagccactga tgaaagacgg 2941 cgcggcgctg ggctactcgc acggtttcaa catcgtcgaa gtgggcgagc agatccgtaa 3001 agatatcacc gtagtgatgg ttgcgccgaa atgcccaggc accgaagtgc gtgaagagta 3061 caaacgtggg ttcggcgtac cgacgctgat tgccgttcac ccggaaaacg atccgaaagg 3121 cgaaggcatg gcgattgcca aagcctgggc ggctgcaacc ggtggtcacc gtgcgggtgt 3181 gctggaatcg tccttcgttg cggaagtgaa atctgatctg atgggcgagc aaaccatcct 3241 gtgcggtatg ttgcaggctg gctctctgct gtgcttcgac aagctggtgg aagaaggtac 3301 cgatccagca tacgcagaaa aactgattca gttcggttgg gaaaccatca ccgaagcact 3361 gaaacagggc ggcatcaccc tgatgatgga ccgtctctct aacccggcga aactgcgtgc 3421 ttatgcgctt tctgaacagc tgaaagagat catggcaccc ctgttccaga aacatatgga 3481 cgacatcatc tccggcgaat tttcttccgg tatgatggcg gactgggcca acgatgataa 3541 gaaactgctg acctggcgtg aagagaccgg caaaaccgcg tttgaaaccg cgccgcagta 3601 tgaaggcaaa atcggcgagc aggagtactt cgataaaggc gtactgatga ttgcgatggt 3661 gaaagcgggc gttgaactgg cgttcgaaac catggtcgat tccggcatca ttgaagagtc 3721 tgcatattat gaatcactgc acgagctgcc gctgattgcc aacaccatcg cccgtaagcg 3781 tctgtacgaa atgaacgtgg ttatctctga taccgctgag tacggtaact atctgttctc 3841 ttacgcttgt gtgccgttgc tgaaaccgtt tatggcagag ctgcaaccgg gcgacctggg 3901 taaagctatt ccggaaggcg cggtagataa cgggcaactg cgtgatgtga acgaagcgat 3961 tcgcagccat gcgattgagc aggtaggtaa gaaactgcgc ggctatatga cagatatgaa 4021 acgtattgct gttgcgggtt aatactagag aaagaggaga aatactagag cccttccagt 4081 gtcgcatttg catgcggaac ttttcgctca ggacggacct tgacaggcat acccgtactc 4141 ataccggtga aaaaccgttt cagtgtcgga tctgtatgcg aaatttctcc ctcagccaga 4201 ccttgcgccg ccatctacgt acgcacaccg gcgagaagcc attccaatgc cgaatatgca 4261 tgcgcaactt cagtctcagg agcaacctgg ggcggcacct aaaaacccac acaggagaaa 4321 aatactagat gcctaagtac cgttccgcca ccaccactca tggtcgtaat atggcgggtg 4381 ctcgtgcgct gtggcgcgcc accggaatga ccgacgccga tttcggtaag ccgattatcg 4441 cggttgtgaa ctcgttcacc caatttgtac cgggtcacgt ccatctgcgc gatctcggta 4501 aactggtcgc cgaacaaatt gaagcggctg gcggcgttgc caaagagttc aacaccattg 4561 cggtggatga tgggattgcc atgggccacg gggggatgct ttattcactg ccatctcgcg 4621 aactgatcgc tgattccgtt gagtatatgg tcaacgccca ctgcgccgac gccatggtct 4681 gcatctctaa ctgcgacaaa atcaccccgg ggatgctgat ggcttccctg cgcctgaata 4741 ttccggtgat ctttgtttcc ggcggcccga tggaggccgg gaaaaccaaa ctttccgatc 4801 agatcatcaa gctcgatctg gtcgatgcga tgatccaggg cgcagacccg aaagtatctg 4861 actcccagag cgatcaggtt gaacgttccg cgtgtccgac ctgcggttcc tgctccggga 4921 tgtttaccgc taactcaatg aactgcctga ccgaagcgct gggcctgtcg cagccgggca 4981 acggctcgct gctggcaacc cacgccgacc gtaagcagct gttccttaat gctggtaaac 5041 gcattgttga attgaccaaa cgttattacg agcaaaacga cgaaagtgca ctgccgcgta 5101 atatcgccag taaggcggcg tttgaaaacg ccatgacgct ggatatcgcg atgggtggat 5161 cgactaacac cgtacttcac ctgctggcgg cggcgcagga agcggaaatc gacttcacca 5221 tgagtgatat cgataagctt tcccgcaagg ttccacagct gtgtaaagtt gcgccgagca 5281 cccagaaata ccatatggaa gatgttcacc gtgctggtgg tgttatcggt attctcggcg 5341 aactggatcg cgcggggtta ctgaaccgtg atgtgaaaaa cgtacttggc ctgacgttgc 5401 cgcaaacgct ggaacaatac gacgttatgc tgacccagga tgacgcggta aaaaatatgt 5461 tccgcgcagg tccagcaggc attcgtacca cacaggcatt ctcgcaagat tgccgttggg 5521 atacgctgga cgacgatcgc gccaatggct gtatccgctc gctggaacac gcctacagca 5581 aagacggcgg cctggcggtg ctctacggta actttgcgga aaacggctgc atcgtgaaaa 5641 cggcaggcgt cgatgacagc atcctcaaat tcaccggccc ggcgaaagtg tacgaaagcc 5701 aggacgatgc ggtagaagcg attctcggcg gtaaagttgt cgccggagat gtggtagtaa 5761 ttcgctatga aggcccgaaa ggcggtccgg ggatgcagga aatgctctac ccaaccagct 5821 tcctgaaatc aatgggtctc ggcaaagcct gtgcgctgat caccgacggt cgtttctctg 5881 gtggcacctc tggtctttcc atcggccacg tctcaccgga agcggcaagc ggcggcagca 5941 ttggcctgat tgaagatggt gacctgatcg ctatcgacat cccgaaccgt ggcattcagt 6001 tacaggtaag cgatgccgaa ctggcggcgc gtcgtgaagc gcaggacgct cgaggtgaca 6061 aagcctggac gccgaaaaat cgtgaacgtc aggtctcctt tgccctgcgt gcttatgcca 6121 gcctggcaac cagcgccgac aaaggcgcgg tgcgcgataa atcgaaactg gggggttaat 6181 actagaggga caagcaatgc ttgccttgaa tagtaacttt tgaatagtga ttcaggaggt 6241 actagatgtc cagattagat aaaagtaaag tgattaacag cgcattagag ctgcttaatg 6301 aggtcggaat cgaaggttta acaacccgta aactcgccca gaagctaggt gtagagcagc 6361 ctacattgta ttggcatgta aaaaataagc gggctttgct cgacgcctta gccattgaga 6421 tgttagatag gcaccatact cacttttgcc ctttagaagg ggaaagctgg caagattttt 6481 tacgtaataa cgctaaaagt tttagatgtg ctttactaag tcatcgcgat ggagcaaaag 6541 tacatttagg tacacggcct acagaaaaac agtatgaaac tctcgaaaat caattagcct 6601 ttttatgcca acaaggtttt tcactagaga atgcattata tgcactcagc gctgtggggc 6661 attttacttt aggttgcgta ttggaagatc aagagcatca agtcgctaaa gaagaaaggg 6721 aaacacctac tactgatagt atgccgccat tattacgaca agctatcgaa ttatttgatc 6781 accaaggtgc agagccagcc ttcttattcg gccttgaatt gatcatatgc ggattagaaa 6841 aacaacttaa atgtgaaagt gggtccgctg caaacgacga aaactacgct ttagtagctt 6901 aataacactg atagtgctag tgtagatcac tactagagcc ggcttatcgg tcagtttcac 6961 ctgatttacg taaaaacccg cttcggcggg tttttgcttt tggaggggca gaaagatgaa 7021 tgactgtcca cgacgctata cccaaaagaa atactagagt ccctatcagt gatagagatt 7081 gacatcccta tcagtgatag agatactgag cactactaga gtcacacagg aaagtactag 7141 atgtatacag taggagatta cctattagac cgattacacg agttaggaat tgaagaaatt 7201 tttggagtcc ctggagacta taacttacaa tttttagatc aaattatttc ccgcaaggat 7261 atgaaatggg tcggaaatgc taatgaatta aatgcttcat atatggctga tggctatgct 7321 cgtactaaaa aagctgccgc atttcttaca acctttggag taggtgaatt gagtgcagtt 7381 aatggattag caggaagtta cgccgaaaat ttaccagtag tagaaatagt gggatcacct 7441 acatcaaaag ttcaaaatga aggaaaattt gttcatcata cgctggctga cggtgatttt 7501 aaacacttta tgaaaatgca cgaacctgtt acagcagctc gaactttact gacagcagaa 7561 aatgcaaccg ttgaaattga ccgagtactt tctgcactat taaaagaaag aaaacctgtc 7621 tatatcaact taccagttga tgttgctgct gcaaaagcag agaaaccctc actccctttg 7681 aaaaaagaaa actcaacttc aaatacaagt gaccaagaga tcttgaacaa aattcaagaa 7741 agcttgaaaa atgccaaaaa accaatcgtg attacaggac atgaaataat tagttttggc 7801 ttagaaaaaa cagtctctca atttatttca aagacaaaac tacctattac gacattaaac 7861 tttggaaaaa gttcagttga tgaagctctc ccttcatttt taggaatcta taatggtaaa 7921 ctctcagagc ctaatcttaa agtattcgtg gaatcagccg acttcatcct gatgcttgga 7981 gttaaactca cagactcttc aacaggagcc ttcactcatc atttaaatga aaataaaatg 8041 atttcactga atatagatga aggaaaaata tttaacgaaa gcatccaaaa ttttgatttt 8101 gaatccctca tctcctctct cttagaccta agcgaaatag aatacaaagg aaaatatatc 8161 gataaaaagc aagaagactt tgttccatca aatgcgcttt tatcacaaga ccgcctatgg 8221 caagcagttg aaaacctaac tcaaagcaat gaaacaatcg ttgctgaaca agggacatca 8281 ttctttggcg cttcatcaat tttcttaaaa ccaaagagtc attttattgg tcaaccctta 8341 tggggatcaa ttggatatac attcccagca gcattaggaa gccaaattgc agataaagaa 8401 agcagacacc ttttatttat tggtgatggt tcacttcaac ttacggtgca agaattagga 8461 ttagcaatca gagaaaaaat taatccaatt tgctttatta tcaataatga tggttataca 8521 gtcgaaagag aaattcatgg accaaatcaa agctacaatg atattccaat gtggaattac 8581 tcaaaattac cagaatcatt tggagcaaca gaagaacgag tagtctcgaa aatcgttaga 8641 actgaaaatg aatttgtgtc tgtcatgaaa gaagctcaag cagatccaaa tagaatgtac 8701 tggattgagt taattttggc aaaagaagat gcaccaaaag tactgaaaaa aatgggcaaa 8761 ctatttgctg aacaaaataa atcataatac tagagccagg catcaaataa aacgaaaggc 8821 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta 8881 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata //