LOCUS       BBa_K887060             3001 bp    DNA     linear       19-May-2024
DEFINITION  B0034(RBS)+HivC(zinc finger)+ilvD+37
ACCESSION   BBa_K887060
VERSION     BBa_K887060.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     misc_feature    21..272
                     /label=BBa_K165006
     CDS             279..2129
                     /label=ilvD
     CDS             279..2129
                     /label=BBa_K539712
     RBS             2184..2189
                     /label=rbs
     misc_feature    2160..2165
                     /label=scar adaptation
     stem_loop       2138..2155
                     /label=stem_loop
     stem_loop       2161..2189
                     /label=predicted stem-loop extending to one position 
                     before start codon
     misc_feature    2138..2189
                     /label=BBa_K115002
     CDS             2199..2815
                     /label=tetR
     misc_feature    2816..2849
                     /label=SsrA
     misc_feature    2856..2880
                     /label=Help:Barcodes
     CDS             2196..2880
                     /label=BBa_C0040
     terminator      2889..3001
                     /label=BBa_J61048
ORIGIN
        1 aaagaggaga aatactagag cccttccagt gtcgcatttg catgcggaac ttttcgctca
       61 ggacggacct tgacaggcat acccgtactc ataccggtga aaaaccgttt cagtgtcgga
      121 tctgtatgcg aaatttctcc ctcagccaga ccttgcgccg ccatctacgt acgcacaccg
      181 gcgagaagcc attccaatgc cgaatatgca tgcgcaactt cagtctcagg agcaacctgg
      241 ggcggcacct aaaaacccac acaggagaaa aatactagat gcctaagtac cgttccgcca
      301 ccaccactca tggtcgtaat atggcgggtg ctcgtgcgct gtggcgcgcc accggaatga
      361 ccgacgccga tttcggtaag ccgattatcg cggttgtgaa ctcgttcacc caatttgtac
      421 cgggtcacgt ccatctgcgc gatctcggta aactggtcgc cgaacaaatt gaagcggctg
      481 gcggcgttgc caaagagttc aacaccattg cggtggatga tgggattgcc atgggccacg
      541 gggggatgct ttattcactg ccatctcgcg aactgatcgc tgattccgtt gagtatatgg
      601 tcaacgccca ctgcgccgac gccatggtct gcatctctaa ctgcgacaaa atcaccccgg
      661 ggatgctgat ggcttccctg cgcctgaata ttccggtgat ctttgtttcc ggcggcccga
      721 tggaggccgg gaaaaccaaa ctttccgatc agatcatcaa gctcgatctg gtcgatgcga
      781 tgatccaggg cgcagacccg aaagtatctg actcccagag cgatcaggtt gaacgttccg
      841 cgtgtccgac ctgcggttcc tgctccggga tgtttaccgc taactcaatg aactgcctga
      901 ccgaagcgct gggcctgtcg cagccgggca acggctcgct gctggcaacc cacgccgacc
      961 gtaagcagct gttccttaat gctggtaaac gcattgttga attgaccaaa cgttattacg
     1021 agcaaaacga cgaaagtgca ctgccgcgta atatcgccag taaggcggcg tttgaaaacg
     1081 ccatgacgct ggatatcgcg atgggtggat cgactaacac cgtacttcac ctgctggcgg
     1141 cggcgcagga agcggaaatc gacttcacca tgagtgatat cgataagctt tcccgcaagg
     1201 ttccacagct gtgtaaagtt gcgccgagca cccagaaata ccatatggaa gatgttcacc
     1261 gtgctggtgg tgttatcggt attctcggcg aactggatcg cgcggggtta ctgaaccgtg
     1321 atgtgaaaaa cgtacttggc ctgacgttgc cgcaaacgct ggaacaatac gacgttatgc
     1381 tgacccagga tgacgcggta aaaaatatgt tccgcgcagg tccagcaggc attcgtacca
     1441 cacaggcatt ctcgcaagat tgccgttggg atacgctgga cgacgatcgc gccaatggct
     1501 gtatccgctc gctggaacac gcctacagca aagacggcgg cctggcggtg ctctacggta
     1561 actttgcgga aaacggctgc atcgtgaaaa cggcaggcgt cgatgacagc atcctcaaat
     1621 tcaccggccc ggcgaaagtg tacgaaagcc aggacgatgc ggtagaagcg attctcggcg
     1681 gtaaagttgt cgccggagat gtggtagtaa ttcgctatga aggcccgaaa ggcggtccgg
     1741 ggatgcagga aatgctctac ccaaccagct tcctgaaatc aatgggtctc ggcaaagcct
     1801 gtgcgctgat caccgacggt cgtttctctg gtggcacctc tggtctttcc atcggccacg
     1861 tctcaccgga agcggcaagc ggcggcagca ttggcctgat tgaagatggt gacctgatcg
     1921 ctatcgacat cccgaaccgt ggcattcagt tacaggtaag cgatgccgaa ctggcggcgc
     1981 gtcgtgaagc gcaggacgct cgaggtgaca aagcctggac gccgaaaaat cgtgaacgtc
     2041 aggtctcctt tgccctgcgt gcttatgcca gcctggcaac cagcgccgac aaaggcgcgg
     2101 tgcgcgataa atcgaaactg gggggttaat actagaggga caagcaatgc ttgccttgaa
     2161 tagtaacttt tgaatagtga ttcaggaggt actagatgtc cagattagat aaaagtaaag
     2221 tgattaacag cgcattagag ctgcttaatg aggtcggaat cgaaggttta acaacccgta
     2281 aactcgccca gaagctaggt gtagagcagc ctacattgta ttggcatgta aaaaataagc
     2341 gggctttgct cgacgcctta gccattgaga tgttagatag gcaccatact cacttttgcc
     2401 ctttagaagg ggaaagctgg caagattttt tacgtaataa cgctaaaagt tttagatgtg
     2461 ctttactaag tcatcgcgat ggagcaaaag tacatttagg tacacggcct acagaaaaac
     2521 agtatgaaac tctcgaaaat caattagcct ttttatgcca acaaggtttt tcactagaga
     2581 atgcattata tgcactcagc gctgtggggc attttacttt aggttgcgta ttggaagatc
     2641 aagagcatca agtcgctaaa gaagaaaggg aaacacctac tactgatagt atgccgccat
     2701 tattacgaca agctatcgaa ttatttgatc accaaggtgc agagccagcc ttcttattcg
     2761 gccttgaatt gatcatatgc ggattagaaa aacaacttaa atgtgaaagt gggtccgctg
     2821 caaacgacga aaactacgct ttagtagctt aataacactg atagtgctag tgtagatcac
     2881 tactagagcc ggcttatcgg tcagtttcac ctgatttacg taaaaacccg cttcggcggg
     2941 tttttgcttt tggaggggca gaaagatgaa tgactgtcca cgacgctata cccaaaagaa
     3001 a
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