LOCUS       BBa_K899003             2938 bp    DNA     linear       01-Jun-2024
DEFINITION  Reporter system for FFT (BBa_K899001)
ACCESSION   BBa_K899003
VERSION     BBa_K899003.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    207..209
                     /label=Start codon
     misc_feature    2052..2054
                     /label=Stop codon
     CDS             207..2054
                     /label=BBa_K899001
     RBS             2063..2075
                     /label=RBS-3\Weak
     RBS             2069..2072
                     /label=RBS
     misc_feature    2063..2075
                     /label=BBa_B0032
     RBS             2063..2075
                     /label=BBa_B0032
     CDS             2082..2801
                     /label=GFP protein
     CDS             2082..2801
                     /label=BBa_E0040
     stem_loop       2821..2864
                     /label=stem_loop
     misc_feature    2810..2889
                     /label=BBa_B0010
     terminator      2810..2889
                     /label=BBa_B0010
     stem_loop       2905..2924
                     /label=T7 TE
     misc_feature    2931..2931
                     /label=stop
     polyA_site      2925..2938
                     /label=polya
     misc_feature    2898..2938
                     /label=BBa_B0012
     terminator      2898..2938
                     /label=BBa_B0012
     misc_feature    2063..2938
                     /label=BBa_E0240
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagatgc aaacccctga accctttaca gaccttgaac
      241 atgaacccca cacaccccta ctggaccacc accacaaccc accaccacaa accaccacaa
      301 aacctttgtt caccagggtt gtgtccggtg tcacctttgt tttattcttc tttggtttcg
      361 ctatcgtatt cattgttctc aaccaacaga actcttctgt tcgtatcgtc accaattcgg
      421 agaaatcttt tataaggtat tcgcagaccg atcgcttgtc gtgggaacgg accgcttttc
      481 attttcagcc tgccaagaat tttatttacg atccagatgg tcagttgttt cacatgggct
      541 ggtaccatat gttctatcaa tacaacccat acgcaccggt ttggggcaat atgtcatggg
      601 gtcactcagt gtccaaagac atgatcaact ggtacgagct gccagtcgct atggtcccga
      661 ccgaatggta tgatatcgag ggcgtcttat ccgggtctac cacggtcctt ccaaacggtc
      721 agatctttgc attgtatact gggaacgcta atgatttttc ccaattacaa tgcaaagctg
      781 tacccgtaaa cttatctgac ccgcttctta ttgagtgggt caagtatgag gataacccaa
      841 tcctgtacac tccaccaggg attgggttaa aggactatcg ggacccgtca acagtctgga
      901 caggtcccga tggaaagcat aggatgatca tgggaactaa acgtggcaat acaggcatgg
      961 tacttgttta ctataccact gattacacga actacgagtt gttggatgag ccgttgcact
     1021 ctgttcccaa caccgatatg tgggaatgcg tcgactttta cccggtttcg ttaaccaatg
     1081 atagtgcact tgatatggcg gcctatgggt cgggtatcaa acacgttatt aaagaaagtt
     1141 gggagggaca tggaatggat tggtattcaa tcgggacata tgacgcgata aatgataaat
     1201 ggactcccga taacccggaa ctagatgtcg gtatcgggtt acggtgcgat tacgggaggt
     1261 tttttgcatc aaagagtctt tatgacccat tgaagaaaag gaggatcact tggggttatg
     1321 ttggagaatc agatagtgct gatcaggacc tctcaagagg atgggctact gtttataatg
     1381 ttggaagaac aattgtacta gatagaaaga ccgggaccca tttacttcat tggcccgttg
     1441 aggaagtcga gagtttgaga tacaacggtc aggagtttaa agagatcaag ctagagcccg
     1501 gttcaatcat tccactcgac ataggcacgg ctacacagtt ggacatagtt gcaacatttg
     1561 aggtggatca agcagcgttg aacgcgacaa gtgaaaccga tgatatttat ggttgcacca
     1621 ctagcttagg tgcagcccaa aggggaagtt tgggaccatt tggtcttgcg gttctagccg
     1681 atggaaccct ttctgagtta actccggttt atttctatat agctaaaaag gcagatggag
     1741 gtgtgtcgac acatttttgt accgataagc taaggtcatc actagattat gatggggaga
     1801 gagtggtgta tgggggcact gttcctgtgt tagatgatga agaactcaca atgaggctat
     1861 tggtggatca ttcgatagtg gaggggtttg cgcaaggagg aaggacggtt ataacatcaa
     1921 gggcgtatcc aacaaaagcg atatacgaac aagcgaagct gttcttgttc aacaacgcca
     1981 caggtacgag tgtgaaagca tctctcaaga tttggcaaat ggcttctgca ccaattcatc
     2041 aatacccttt ttaatactag agtcacacag gaaagtacta gatgcgtaaa ggagaagaac
     2101 ttttcactgg agttgtccca attcttgttg aattagatgg tgatgttaat gggcacaaat
     2161 tttctgtcag tggagagggt gaaggtgatg caacatacgg aaaacttacc cttaaattta
     2221 tttgcactac tggaaaacta cctgttccat ggccaacact tgtcactact ttcggttatg
     2281 gtgttcaatg ctttgcgaga tacccagatc atatgaaaca gcatgacttt ttcaagagtg
     2341 ccatgcccga aggttatgta caggaaagaa ctatattttt caaagatgac gggaactaca
     2401 agacacgtgc tgaagtcaag tttgaaggtg atacccttgt taatagaatc gagttaaaag
     2461 gtattgattt taaagaagat ggaaacattc ttggacacaa attggaatac aactataact
     2521 cacacaatgt atacatcatg gcagacaaac aaaagaatgg aatcaaagtt aacttcaaaa
     2581 ttagacacaa cattgaagat ggaagcgttc aactagcaga ccattatcaa caaaatactc
     2641 caattggcga tggccctgtc cttttaccag acaaccatta cctgtccaca caatctgccc
     2701 tttcgaaaga tcccaacgaa aagagagacc acatggtcct tcttgagttt gtaacagctg
     2761 ctgggattac acatggcatg gatgaactat acaaataata atactagagc caggcatcaa
     2821 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
     2881 aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata
//