LOCUS       BBa_K902001             1892 bp    DNA     linear       28-May-2024
DEFINITION  Beta-glucuronidase (uidA) constitutive generator
ACCESSION   BBa_K902001
VERSION     BBa_K902001.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     promoter        1..74
                     /label=BBa_J13002
     misc_feature    81..83
                     /label=start
     misc_feature    1890..1892
                     /label=stop
     CDS             81..1892
                     /label=UidA
     CDS             81..1892
                     /label=BBa_K902000
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgttacgtc ctgtagaaac cccaacccgt gaaatcaaaa
      121 aactcgacgg cctgtgggca ttcagtctgg atcgcgaaaa ctgtggaatt gatcagcgtt
      181 ggtgggaaag cgcgttacaa gaaagccggg caattgctgt gccaggcagt tttaacgatc
      241 agttcgccga tgcagatatt cgtaattatg cgggcaacgt ctggtatcag cgcgaagtct
      301 ttataccgaa aggttgggca ggccagcgta tcgtgctgcg tttcgatgcg gtcactcatt
      361 acggcaaagt gtgggtcaat aatcaggaag tgatggagca tcagggcggc tatacgccat
      421 ttgaagccga tgtcacgccg tatgttattg ccgggaaaag tgtacgtatc accgtttgtg
      481 tgaacaacga actgaactgg cagactatcc cgccgggaat ggtgattacc gacgaaaacg
      541 gcaagaaaaa gcagtcttac ttccatgatt tctttaacta tgccgggatc catcgcagcg
      601 taatgctcta caccacgccg aacacctggg tggacgatat caccgtggtg acgcatgtcg
      661 cgcaagactg taaccacgcg tctgttgact ggcaggtggt ggccaatggt gatgtcagcg
      721 ttgaactgcg tgatgcggat caacaggtgg ttgcaactgg acaaggcact agcgggactt
      781 tgcaagtggt gaatccgcac ctctggcaac cgggtgaagg ttatctctat gaactgtgcg
      841 tcacagccaa aagccagaca gagtgtgata tctacccgct tcgcgtcggc atccggtcag
      901 tggcagtgaa gggcgaacag ttcctgatta accacaaacc gttctacttt actggctttg
      961 gtcgtcatga agatgcggac ttgcgtggca aaggattcga taacgtgctg atggtgcacg
     1021 accacgcatt aatggactgg attggggcca actcctaccg tacctcgcat tacccttacg
     1081 ctgaagagat gctcgactgg gcagatgaac atggcatcgt ggtgattgat gaaactgctg
     1141 ctgtcggctt taacctctct ttaggcattg gtttcgaagc gggcaacaag ccgaaagaac
     1201 tgtacagcga agaggcagtc aacggggaaa ctcagcaagc gcacttacag gcgattaaag
     1261 agctgatagc gcgtgacaaa aaccacccaa gcgtggtgat gtggagtatt gccaacgaac
     1321 cggatacccg tccgcaaggt gcacgggaat atttcgcgcc actggcggaa gcaacgcgta
     1381 aactcgaccc gacgcgtccg atcacctgcg tcaatgtaat gttctgcgac gctcacaccg
     1441 ataccatcag cgatctcttt gatgtgctgt gcctgaaccg ttattacgga tggtatgtcc
     1501 aaagcggcga tttggaaacg gcagagaagg tactggaaaa agaacttctg gcctggcagg
     1561 agaaactgca tcagccgatt atcatcaccg aatacggcgt ggatacgtta gccgggctgc
     1621 actcaatgta caccgacatg tggagtgaag agtatcagtg tgcatggctg gatatgtatc
     1681 accgcgtctt tgatcgcgtc agcgccgtcg tcggtgaaca ggtatggaat ttcgccgatt
     1741 ttgcgacctc gcaaggcata ttgcgcgttg gcggtaacaa gaaagggatc ttcactcgcg
     1801 accgcaaacc gaagtcggcg gcttttctgc tgcaaaaacg ctggactggc atgaacttcg
     1861 gtgaaaaacc gcagcaggga ggcaaacaat ga
//