LOCUS       BBa_K902041             1645 bp    DNA     linear       27-May-2024
DEFINITION  Constitutive AmdA Generator
ACCESSION   BBa_K902041
VERSION     BBa_K902041.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     promoter        1..74
                     /label=BBa_J13002
     misc_feature    81..83
                     /label=ATG Start Codon
     misc_feature    1643..1645
                     /label=TAG Stop Codon
     misc_feature    152..152
                     /label=A --> G
     CDS             81..1645
                     /label=BBa_K902035
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atggcgacaa tccgacctga cgacaatgca atagacaccg
      121 ccgcaaggca ttacggcatc actctcgacc agtcagcccg gctcgagtgg ccggcactga
      181 tcgacggagc actgggctcc tacgacgtcg tcgaccagtt gtacgccgac gaggcaaccc
      241 cgccgaccac gtcacgtgag cacacggtgc caacagcgag cgaaaatcct ttgagcgctt
      301 ggtatgtgac cacaagcatc ccgccgacgt cggacggcgt cctgaccggc cgacgcgtgg
      361 cgatcaagga caacgtgacc gtggccggag ttccgatgat gaacgggtct cggacagtag
      421 aggggttcac tccgtctcgc gacgcgactg tggtcactcg actactggcg gccggtgcaa
      481 ccgtcgcggg caaagctgtg tgtgaggacc tgtgtttctc cggttcgagc ttcacaccgg
      541 caagcggacc ggtccgcaat ccatgggacc cacagcgtga agcaggtgga tcatccggtg
      601 gcagtgcggc tctcgtcgca aacggtgacg tcgattttgc catcggcggg gatcagggtg
      661 gatcgatccg gatcccggcg gcattctgcg gcgtcgtcgg acacaagccg acgttcgggc
      721 tcgtcccgta taccggtgca ttccccatcg agcgaacaat cgaccatctc ggcccgatca
      781 cacgcacggt ccacgatgcc gcactgatgc tctcggtcat cgccggtcgc gacggtaacg
      841 acccacgcca agccgacagc gtcgaagcag gtgactatct gtccaccctc gactccgatg
      901 tggatggtct gcgaatcggg atcgttcgag aaggtttcgg gcacgcggtc tcacagcccg
      961 aggtcgacga cgcagtccgc gcagcggcac acagtctggc cgaaatcggt tgcacggtag
     1021 aggaagtaaa catcccgtgg cactacatgc tttccacatc tggaacgtga tcgccacgga
     1081 cggtggtgcc taccagatgt tggacggcaa cggatacggc atgaacgccg aaggtttgta
     1141 cgatccggaa ctgatggcac actttgcttc tcgacgcctt cagcacgccg acgctctgtc
     1201 cgaaaccgtc aaactggtgg ctctgaccgg ccaccacggc atcaccaccc tcggcggcgc
     1261 gagctacggc aaagcccgga acctcgtacc gctcgcccgc gccgcctacg acactgcctt
     1321 gagacaattc gacgtcctgg tgatgcccac actgccctac gtcgcatccg aattgccggc
     1381 gaacgacgtg gatcgtgcaa ccttcatcac gaaagctctc gggatgatcg ccaacacagc
     1441 accattcgac gtgaccggac atccgtccct gtccgttccc gccggcctgg tgaacgggct
     1501 tccggtcgga atgatgatca ccggcaagac cttcgacgat gcgacggtcc tccgggtcgg
     1561 gcgcgcattc gaaaagcttc gcggcgcgtt tccgacgcct gccgaccaca tctccgactc
     1621 tgcaccacaa ctcagcctca cctag
//