LOCUS       BBa_K902081             2982 bp    DNA     linear       01-Jun-2024
DEFINITION  4S Desulfurization Pathway Optimization Circuit 
            (PlacI/hpaC/RBS/katGLAA)
ACCESSION   BBa_K902081
VERSION     BBa_K902081.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    1..220
                     /label=BBa_J04500
     misc_feature    227..229
                     /label=start
     CDS             227..739
                     /label=HpaC
     misc_feature    737..739
                     /label=stop
     CDS             227..739
                     /label=BBa_K902057
     misc_feature    1..739
                     /label=BBa_K902058
     misc_feature    752..755
                     /label=conserved
     RBS             748..759
                     /label=BBa_B0034
     misc_feature    963..963
                     /label=C->T
     CDS             766..2982
                     /label=BBa_K137068
     misc_feature    748..2982
                     /label=BBa_K902059
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgc aattagatga
      241 acaacgcctg cgctttcgtg acgcaatggc cagcctgtcg gcagcggtaa atattatcac
      301 caccgagggc gacgccggac aatgcgggat tacggcaacg gccgtctgct cggtcacgga
      361 tacaccacca tcgctgatgg tgtgcattaa cgccaacagt gcgatgaacc cggtttttca
      421 gggcaacggt aagttgtgcg tcaacgtcct caaccatgag caggaactga tggcacgcca
      481 cttcgcgggc atgacaggca tggcgatgga agagcgtttt agcctctcat gctggcaaaa
      541 aggtccgctg gcgcagccgg tgctaaaagg ttcgctggcc agtcttgaag gtgagatccg
      601 cgatgtgcag gcaattggca cacatctggt gtatctggtg gagattaaaa acatcatcct
      661 cagtgcagaa ggtcacggac ttatctactt taaacgccgt ttccatccgg tgatgctgga
      721 aatggaagct gcgatttaat actagagaaa gaggagaaat actagatgag cacgtcagac
      781 gatatccata acaccacagc cactggcaaa tgcccgttcc atcagggcgg tcacgaccag
      841 agtgcggggg cgggcacaac cactcgcgac tggtggccaa atcaacttcg tgttgacctg
      901 ttaaaccaac attctaatcg ttctaaccca ctgggtgagg actttgacta ccgcaaagaa
      961 tttagcaaat tagattacta cggcctgaaa aaagatctga aagccctgtt gacagaatct
     1021 caaccgtggt ggccagccga ctggggcagt tacgccggtc tgtttattcg tatggcctgg
     1081 cacggcgcgg ggacttaccg ttcaatcgat ggacgcggtg gcgcgggtcg tggtcagcaa
     1141 cgttttgcac cgctgaactc ctggccggat aacgtaagcc tcgataaagc gcgtcgcctg
     1201 ttgtggccaa tcaaacagaa atatggtcag aaaatctcct gggccgacct gtttatcctc
     1261 gcgggtaacg tggcgctaga aaactccggc ttccgtacct tcggttttgg tgccggtcgt
     1321 gaagacgtct gggaaccgga tctggatgtt aactggggtg atgaaaaagc ctggctgact
     1381 caccgtcatc cggaagcgct ggcgaaagca ccgctgggtg caaccgagat gggtctgatt
     1441 tacgttaacc cggaaggccc ggatcacagc ggcgaaccgc tttctgcggc agcagctatc
     1501 cgcgcgacct tcggcaacat gggcatgaac gacgaagaaa ccgtggcgct gattgcgggt
     1561 ggtcatacgc tgggtaaaac ccacggtgcc ggtccgacat caaatgtagg tcctgatcca
     1621 gaagctgcac cgattgaaga acaaggttta ggttgggcga gcacttacgg cagcggcgtt
     1681 ggcgcagatg ccattacctc tggtctggaa gtagtctgga cccagacgcc gacccagtgg
     1741 agcaactatt tcttcgagaa cctgttcaag tatgagtggg tacagacccg cagcccggct
     1801 ggcgcaatcc agttcgaagc ggtagacgca ccggaaatta tcccggatcc gtttgatccg
     1861 tcgaagaaac gtaaaccgac aatgctggtg accgacctga cgctgcgttt tgatcctgag
     1921 ttcgagaaga tctctcgtcg tttcctcaac gatccgcagg cgttcaacga agcctttgcc
     1981 cgtgcctggt tcaaactgac gcacagggat atggggccga aatctcgcta catcgggccg
     2041 gaagtgccga aagaagatct gatctggcaa gatccgctgc cgcagccgat ctacaacccg
     2101 accgagcagg acattatcga tctgaaattc gcgattgcgg attctggtct gtctgttagt
     2161 gagctggtat cggtggcctg ggcatctgct tctaccttcc gtggtggcga caaacgcggt
     2221 ggtgccaacg gtgcgcgtct ggcattaatg ccgcagcgcg actgggatgt gaacgccgca
     2281 gccgttcgtg ctctgcctgt tctggagaaa atccagaaag agtctggtaa agcctcgctg
     2341 gcggatatca tagtgctggc tggtgtggtt ggtgttgaga aagccgcaag cgccgcaggt
     2401 ttgagcattc atgtaccgtt tgcgccgggt cgcgttgatg cgcgtcagga tcagactgac
     2461 attgagatgt ttgagctgct ggagccaatt gctgacggtt tccgtaacta tcgcgctcgt
     2521 ctggacgttt ccaccaccga gtcactgctg atcgacaaag cacagcaact gacgctgacc
     2581 gcgccggaaa tgactgcgct ggtgggcggc atgcgtgtac tgggtgccaa cttcgatggc
     2641 agcaaaaacg gcgtcttcac tgaccgcgtt ggcgtattga gcaatgactt cttcgtgaac
     2701 ttgctggata tgcgttacga gtggaaagcg accgacgaat cgaaagagct gttcgaaggc
     2761 cgtgaccgtg aaaccggcga agtgaaattt acggccagcc gtgcggatct ggtgtttggt
     2821 tctaactccg tcctgcgtgc ggtggcggaa gtttacgcca gtagcgatgc ccacgagaag
     2881 tttgttaaag acttcgtggc ggcatgggtg aaagtgatga acctcgaccg tttcgacctg
     2941 ctggctgcta acgacgaaaa ctacgctctg gctgcttaat aa
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