Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Oliver Meacock
Date created: 2012-09-11 11:00:00
Date modified: 2015-05-08 01:13:45
Fluoride Sensitive Riboswitch
| Types | DnaRegion |
| Roles | engineered_region Measurement |
| Sequences | BBa_K911003_sequence (Version 1) |
Description
Riboswitch that is highly sensitive to the F- ion. We have characterized this part in three different chassis: TOP10 e.coli, Laboratory strain bacillus subtilis and a strain of bacillus subtilis with its normal fluoride riboswitch knocked out (kindly provided by the Breaker lab in Yale). Results of Miller assays for these three chassis are shown below.Notes
Because this is a positive regulator (it ceases transcriptional termination in the presence of F- ions), it was decided that it should be placed upstream of a direct reporter, in this case lacZ as our reporter.Source
Bacillus Cereus Genome| Sequence Annotation | Location | Component / Role(s) |
| Lysine promoter Fluoride riboswitch | 1,43 61,139 | promoter feature/promoter feature/misc sequence_feature |