LOCUS       BBa_K912035             3585 bp    DNA     linear       14-May-2024
DEFINITION  pH Inducible GFP system (s)
ACCESSION   BBa_K912035
VERSION     BBa_K912035.1  
FEATURES             Location/Qualifiers
     misc_feature    1249..1251
                     /label=gadC Start
     promoter        141..158
                     /label=PgadR
     CDS             275..1105
                     /label=gadR
     promoter        1163..1181
                     /label=Pgad
     RBS             1237..1245
                     /label=RBS_gadC
     RBS             264..269
                     /label=RBS_gadR
     promoter        1..1251
                     /label=BBa_K559011
     CDS             1258..1977
                     /label=GFP protein
     CDS             1258..1977
                     /label=BBa_E0040
     promoter        1986..2020
                     /label=BBa_J23104
     CDS             2027..3448
                     /label=BBa_K912005
     stem_loop       3468..3511
                     /label=stem_loop
     misc_feature    3457..3536
                     /label=BBa_B0010
     terminator      3457..3536
                     /label=BBa_B0010
     stem_loop       3552..3571
                     /label=T7 TE
     misc_feature    3578..3578
                     /label=stop
     polyA_site      3572..3585
                     /label=polya
     misc_feature    3545..3585
                     /label=BBa_B0012
     terminator      3545..3585
                     /label=BBa_B0012
ORIGIN
        1 tgagcgttgt ataagctttt atgtctttct atatcaactt ttaatagaaa tataaagtaa
       61 tataaatgtt tttataataa attatgtgag atatattttt ttgtccgtac tggtatagat
      121 ttgacgatta agtcttaaat aagttataat ctcaattgcg taatttctta aatacagaaa
      181 taacaactac attggtagac tgattaaaaa gtgtacttga tgaactgtta taaaccttaa
      241 aaaaataaaa ataatagttt gggggatgtt aaagatgtat aaaaaatatg gagattgttt
      301 taaaaagttg cgaaaccaaa agaatttagg gttatcatac tttagtaaac ttggaataga
      361 ccgttcaaat atatcaagat ttgaacatgg aaaatgtatg atgagttttg agcgtataga
      421 tttgatgtta gaagaaatgc aagttccgtt atctgagtac gaattgattg taaataattt
      481 tatgccgaat ttccaagaat tttttatatt agaattggaa aaagctgaat ttagccaaaa
      541 tcgagataaa ataaaagagt tgtattctga ggtcaaagaa acggggaatc atttactgac
      601 ggttaccgtg aaaacgaagc ttgggaatat aagtcagaca gaagttaaag aaattgaagc
      661 ttatctttgc aatattgaag agtggggata ttttgaactt actttatttt attttgtatc
      721 tgattatctc aatgtcaatc aattagaatt gctgcttttt aattttgata aaagatgtga
      781 aaattactgt agagtcttaa aatatagaag gagactattg caaatagcct ataaaagtgt
      841 tgcgatatac gcggctaaag gagaaagaaa aaaagccgaa aatattttag aaatgactaa
      901 aaaatatcga actgtgggag tcgatttata ttcagaagta ttaagacatc ttgctagagc
      961 tatcattatt tttaattttg aaaatgcaga gattggggaa gaaaaaataa attatgctct
     1021 tgagattttg gaagaatttg gaggaaagaa gataaaagaa ttttatcaga ataaaatgga
     1081 aaagtatttg aaaaggtcaa tttagtctct tttgagctgt tgctttaaag caacagctca
     1141 aaagagattt tctttattcc agagcatata ctagagggtg aagataggtt gtctgaagca
     1201 ttataacttg tcttttaaaa aattcaatca taaatataag gaggtatgat gtactagatg
     1261 cgtaaaggag aagaactttt cactggagtt gtcccaattc ttgttgaatt agatggtgat
     1321 gttaatgggc acaaattttc tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa
     1381 cttaccctta aatttatttg cactactgga aaactacctg ttccatggcc aacacttgtc
     1441 actactttcg gttatggtgt tcaatgcttt gcgagatacc cagatcatat gaaacagcat
     1501 gactttttca agagtgccat gcccgaaggt tatgtacagg aaagaactat atttttcaaa
     1561 gatgacggga actacaagac acgtgctgaa gtcaagtttg aaggtgatac ccttgttaat
     1621 agaatcgagt taaaaggtat tgattttaaa gaagatggaa acattcttgg acacaaattg
     1681 gaatacaact ataactcaca caatgtatac atcatggcag acaaacaaaa gaatggaatc
     1741 aaagttaact tcaaaattag acacaacatt gaagatggaa gcgttcaact agcagaccat
     1801 tatcaacaaa atactccaat tggcgatggc cctgtccttt taccagacaa ccattacctg
     1861 tccacacaat ctgccctttc gaaagatccc aacgaaaaga gagaccacat ggtccttctt
     1921 gagtttgtaa cagctgctgg gattacacat ggcatggatg aactatacaa ataataatac
     1981 tagagttgac agctagctca gtcctaggta ttgtgctagc tactagatga aaactgatac
     2041 tccctcttta gaaacaccgc aggccgcgcg cctgcgacgc agacaactga ttcgccaact
     2101 tcttgagcgc gataaaaccc cgttagccat tttgtttatg gcggcagtcg tcggcacgct
     2161 tgttgggctg gcagcggttg cttttgacaa aggtgtcgcc tggttgcaga accaacgtat
     2221 gggggcgctg gtacatactg ctgataatta tccgcttctg ttaaccgtcg cttttctctg
     2281 ttcggcggtg ctggcgatgt ttggctactt tttggtgcgc aaatacgcgc cggaagcagg
     2341 tggttcgggg atcccggaaa ttgaaggggc gctggaagat caacgtcccg ttcgctggtg
     2401 gcgtgtattg ccggtgaagt tctttggcgg gctggggaca ctcggcggag gcatggtgtt
     2461 ggggcgcgaa gggccaaccg tgcagatcgg cggtaacatt ggccgtatgg tgcttgatat
     2521 tttccgcctg aaaggtgacg aagctcgcca tacgctgctg gcaaccggtg ctgctgcggg
     2581 gctggctgcg gcctttaacg cgccgctggc gggtattttg tttattatcg aagagatgcg
     2641 tccgcagttt cgctatacgt taatttcgat taaagcggta tttattggtg tcattatgtc
     2701 gaccattatg taccggattt ttaatcatga agttgcgttg attgacgtcg gtaaactttc
     2761 tgatgcgccg cttaatacgc tgtggcttta tctgatcctc ggtattattt ttggcatttt
     2821 cggccctatt tttaataaat gggtgctggg gatgcaggat ttgctgcacc gtgtgcacgg
     2881 cggcaatatt accaaatggg tgctaatggg cggtgcgatt ggcggtctgt gtggattgct
     2941 ggggtttgtg gcaccagcaa cgtcgggcgg cggttttaac ctgattccta tcgctaccgc
     3001 ggggaatttc agcatgggaa tgctggtgtt tatcttcgtc gcgcgggtca ttaccacctt
     3061 actctgcttc tcttccggcg cgccgggcgg tatttttgcc ccgatgctgg cgctgggtac
     3121 tgtgctggga accgctttcg gaatggttgc cgttgagctg tttccgcaat atcaccttga
     3181 ggcggggacg tttgctattg ccggaatggg ggcattactg gcggcatcta ttcgcgcgcc
     3241 gttaacgggg atcattctgg ttctggagat gaccgataac taccagctca ttttgccaat
     3301 gattattacc ggtcttggcg caacactatt agcgcaattt accggcggga aaccgctata
     3361 ctcggcgatt cttgcgcgca cgctggcaaa acaggaagct gagcaactgg cgcgaagcaa
     3421 ggccgcatca gccagcgaga atacttgata ctagagccag gcatcaaata aaacgaaagg
     3481 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact
     3541 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttata
//