BBa_K914003

BBa_K914003 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K914003
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Denis Samuylov
Date created: 2012-09-19 11:00:00
Date modified: 2015-05-08 01:13:45

L-rhamnose-inducible promoter (pRha)



Types
DnaRegion

Roles
promoter

Regulatory

Sequences BBa_K914003_sequence (Version 1)

Description

L-rhamnose-inducible promoter is capable of high-level recombinant protein expression in the presence of L-rhamnose, it is also tightly regulated in the absence of L-rhamnose by the addition of D-glucose.

Notes

One base pair modified (90: A -> T) to avoid EcoRI site. Mutation made in a less conserved base pair.

Source

Amplification of the plasmid pJOE3075 (Dr. Altenbuchner).

Sequence Annotation Location Component / Role(s)
pRha
1,122
promoter feature/promoter
igem#experience
Works
 
igem#partStatus
Released HQ 2013
igem#sampleStatus
In stock
igem#status
Available
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K914003/1