LOCUS       BBa_K914005              794 bp    DNA     linear       01-Jun-2024
DEFINITION  Meganuclease I-SceI controlled by pLac
ACCESSION   BBa_K914005
VERSION     BBa_K914005.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     RBS             64..76
                     /label=RBS-3\Weak
     RBS             70..73
                     /label=RBS
     misc_feature    64..76
                     /label=BBa_B0032
     RBS             64..76
                     /label=BBa_B0032
     CDS             83..794
                     /label=I-SceI
     CDS             83..794
                     /label=BBa_K914006
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagtcacaca ggaaagtact agatgaaaaa catcaaaaaa aaccaggtaa tgaacctggg
      121 tccgaactct aaactgctga aagaatacaa atcccagctg atcgaactga acatcgaaca
      181 gttcgaagca ggtatcggtc tgatcctggg tgatgcttac atccgttctc gtgatgaagg
      241 taaaacctac tgtatgcagt tcgagtggaa aaacaaagca tacatggacc acgtatgtct
      301 gctgtacgat cagtgggtac tgtccccgcc gcacaaaaaa gaacgtgtta accacctggg
      361 taacctggta atcacctggg gcgcccagac tttcaaacac caagctttca acaaactggc
      421 taacctgttc atcgttaaca acaaaaaaac catcccgaac aacctggttg aaaactacct
      481 gaccccgatg tctctggcat actggttcat ggatgatggt ggtaaatggg attacaacaa
      541 aaactctacc aacaaatcga tcgtactgaa cacccagtct ttcactttcg aagaagtaga
      601 atacctggtt aagggtctgc gtaacaaatt ccaactgaac tgttacgtaa aaatcaacaa
      661 aaacaaaccg atcatctaca tcgattctat gtcttacctg atcttctaca acctgatcaa
      721 accgtacctg atcccgcaga tgatgtacaa actgccgaac actatctcct ccgaaacttt
      781 cctgaaataa taag
//