LOCUS       BBa_K914007              869 bp    DNA     linear       01-Jun-2024
DEFINITION  Meganuclease I-SceI controlled by pBad
ACCESSION   BBa_K914007
VERSION     BBa_K914007.1  
FEATURES             Location/Qualifiers
     promoter        1..130
                     /label=BBa_I13453
     RBS             139..151
                     /label=RBS-3\Weak
     RBS             145..148
                     /label=RBS
     misc_feature    139..151
                     /label=BBa_B0032
     RBS             139..151
                     /label=BBa_B0032
     CDS             158..869
                     /label=I-SceI
     CDS             158..869
                     /label=BBa_K914006
ORIGIN
        1 acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta tccataagat
       61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt
      121 ttgggctagc tactagagtc acacaggaaa gtactagatg aaaaacatca aaaaaaacca
      181 ggtaatgaac ctgggtccga actctaaact gctgaaagaa tacaaatccc agctgatcga
      241 actgaacatc gaacagttcg aagcaggtat cggtctgatc ctgggtgatg cttacatccg
      301 ttctcgtgat gaaggtaaaa cctactgtat gcagttcgag tggaaaaaca aagcatacat
      361 ggaccacgta tgtctgctgt acgatcagtg ggtactgtcc ccgccgcaca aaaaagaacg
      421 tgttaaccac ctgggtaacc tggtaatcac ctggggcgcc cagactttca aacaccaagc
      481 tttcaacaaa ctggctaacc tgttcatcgt taacaacaaa aaaaccatcc cgaacaacct
      541 ggttgaaaac tacctgaccc cgatgtctct ggcatactgg ttcatggatg atggtggtaa
      601 atgggattac aacaaaaact ctaccaacaa atcgatcgta ctgaacaccc agtctttcac
      661 tttcgaagaa gtagaatacc tggttaaggg tctgcgtaac aaattccaac tgaactgtta
      721 cgtaaaaatc aacaaaaaca aaccgatcat ctacatcgat tctatgtctt acctgatctt
      781 ctacaacctg atcaaaccgt acctgatccc gcagatgatg tacaaactgc cgaacactat
      841 ctcctccgaa actttcctga aataataag
//