LOCUS       BBa_K914014             3312 bp    DNA     linear       17-May-2024
DEFINITION  Pbad/AraC RBS LacI[LVA(-)] Plac GFP
ACCESSION   BBa_K914014
VERSION     BBa_K914014.1  
FEATURES             Location/Qualifiers
     misc_feature    1..1114
                     /label=BBa_I13458
     promoter        1123..1252
                     /label=BBa_I13453
     misc_feature    1265..1268
                     /label=conserved
     RBS             1261..1272
                     /label=BBa_B0034
     CDS             1279..2358
                     /label=LacI (Lva-, N-terminal deletion) regulatory protein
     misc_feature    1279..1281
                     /label=start
     misc_feature    2359..2361
                     /label=stop
     misc_feature    2362..2364
                     /label=stop
     CDS             1279..2364
                     /label=BBa_K143033
     promoter        2389..2394
                     /label=-35
     promoter        2416..2421
                     /label=-10
     promoter        2373..2428
                     /label=BBa_K091110
     RBS             2437..2449
                     /label=RBS-3\Weak
     RBS             2443..2446
                     /label=RBS
     misc_feature    2437..2449
                     /label=BBa_B0032
     RBS             2437..2449
                     /label=BBa_B0032
     CDS             2456..3175
                     /label=GFP protein
     CDS             2456..3175
                     /label=BBa_E0040
     stem_loop       3195..3238
                     /label=stem_loop
     misc_feature    3184..3263
                     /label=BBa_B0010
     terminator      3184..3263
                     /label=BBa_B0010
     stem_loop       3279..3298
                     /label=T7 TE
     misc_feature    3305..3305
                     /label=stop
     polyA_site      3299..3312
                     /label=polya
     misc_feature    3272..3312
                     /label=BBa_B0012
     terminator      3272..3312
                     /label=BBa_B0012
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctattactag agacattgat tatttgcacg
     1141 gcgtcacact ttgctatgcc atagcatttt tatccataag attagcggat cctacctgac
     1201 gctttttatc gcaactctct actgtttctc cataccgttt ttttgggcta gctactagag
     1261 aaagaggaga aatactagat gaaaccagta acgttatacg atgtcgcaga gtatgccggt
     1321 gtctcttatc agaccgtttc ccgcgtggtg aaccaggcca gccacgtttc tgcgaaaacg
     1381 cgggaaaaag tggaagcggc gatggcggag ctgaattaca ttcccaaccg cgtggcacaa
     1441 caactggcgg gcaaacagtc gttgctgatt ggcgttgcca cctccagtct ggccctgcac
     1501 gcgccgtcgc aaattgtcgc ggcgattaaa tctcgcgccg atcaactggg tgccagcgtg
     1561 gtggtgtcga tggtagaacg aagcggcgtc gaagcctgta aaacggcggt gcacaatctt
     1621 ctcgcgcaac gcgtcagtgg gctgatcatt aactatccgc tggatgacca ggatgccatt
     1681 gctgtggaag ctgcctgcac taatgttccg gcgttatttc ttgatgtctc tgaccagaca
     1741 cccatcaaca gtattatttt ctcccatgaa gacggtacgc gactgggcgt ggagcatctg
     1801 gtcgcattgg gtcaccagca aatcgcgctg ttagcgggcc cattaagttc tgtctcggcg
     1861 cgtctgcgtc tggctggctg gcataaatat ctcactcgca atcaaattca gccgatagcg
     1921 gaacgggaag gcgactggag tgccatgtcc ggttttcaac aaaccatgca aatgctgaat
     1981 gagggcatcg ttcccactgc gatgctggtt gccaacgatc agatggcgct gggcgcaatg
     2041 cgcgccatta ccgagtccgg gctgcgcgtt ggtgcggata tctcggtagt gggatacgac
     2101 gataccgaag acagctcatg ttatatcccg ccgtcaacca ccatcaaaca ggattttcgc
     2161 ctgctggggc aaaccagcgt ggaccgcttg ctgcaactct ctcagggcca ggcggtgaag
     2221 ggcaatcagc tgttgcccgt ctcactggtg aaaagaaaaa ccaccctggc gcccaatacg
     2281 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     2341 cgactggaaa gcgggcagta ataatactag agcgttgaca ccatcgaatg gcgcaaaacc
     2401 tttcgcggta tggcatgata gcgcccggta ctagagtcac acaggaaagt actagatgcg
     2461 taaaggagaa gaacttttca ctggagttgt cccaattctt gttgaattag atggtgatgt
     2521 taatgggcac aaattttctg tcagtggaga gggtgaaggt gatgcaacat acggaaaact
     2581 tacccttaaa tttatttgca ctactggaaa actacctgtt ccatggccaa cacttgtcac
     2641 tactttcggt tatggtgttc aatgctttgc gagataccca gatcatatga aacagcatga
     2701 ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa agaactatat ttttcaaaga
     2761 tgacgggaac tacaagacac gtgctgaagt caagtttgaa ggtgataccc ttgttaatag
     2821 aatcgagtta aaaggtattg attttaaaga agatggaaac attcttggac acaaattgga
     2881 atacaactat aactcacaca atgtatacat catggcagac aaacaaaaga atggaatcaa
     2941 agttaacttc aaaattagac acaacattga agatggaagc gttcaactag cagaccatta
     3001 tcaacaaaat actccaattg gcgatggccc tgtcctttta ccagacaacc attacctgtc
     3061 cacacaatct gccctttcga aagatcccaa cgaaaagaga gaccacatgg tccttcttga
     3121 gtttgtaaca gctgctggga ttacacatgg catggatgaa ctatacaaat aataatacta
     3181 gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
     3241 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt
     3301 tctgcgttta ta
//