LOCUS       BBa_K934026             1304 bp    DNA     linear       17-May-2024
DEFINITION  Plux-LacI
ACCESSION   BBa_K934026
VERSION     BBa_K934026.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=LuxR/HSL
     promoter        20..25
                     /label=-35
     promoter        42..47
                     /label=-10
     misc_feature    53..53
                     /label=start
     misc_feature    1..55
                     /label=BBa_R0062
     promoter        1..55
                     /label=BBa_R0062
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     CDS             82..1167
                     /label=BBa_I732100
     stem_loop       1187..1230
                     /label=stem_loop
     misc_feature    1176..1255
                     /label=BBa_B0010
     terminator      1176..1255
                     /label=BBa_B0010
     stem_loop       1271..1290
                     /label=T7 TE
     misc_feature    1297..1297
                     /label=stop
     polyA_site      1291..1304
                     /label=polya
     misc_feature    1264..1304
                     /label=BBa_B0012
     terminator      1264..1304
                     /label=BBa_B0012
     misc_feature    64..1304
                     /label=BBa_I732820
ORIGIN
        1 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga ataaatacta
       61 gagaaagagg agaaatacta gatgaaacca gtaacgttat acgatgtcgc agagtatgcc
      121 ggtgtctctt atcagaccgt ttcccgcgtg gtgaaccagg ccagccacgt ttctgcgaaa
      181 acgcgggaaa aagtggaagc ggcgatggcg gagctgaatt acattcccaa ccgcgtggca
      241 caacaactgg cgggcaaaca gtcgttgctg attggcgttg ccacctccag tctggccctg
      301 cacgcgccgt cgcaaattgt cgcggcgatt aaatctcgcg ccgatcaact gggtgccagc
      361 gtggtggtgt cgatggtaga acgaagcggc gtcgaagcct gtaaagcggc ggtgcacaat
      421 cttctcgcgc aacgcgtcag tgggctgatc attaactatc cgctggatga ccaggatgcc
      481 attgctgtgg aagctgcctg cactaatgtt ccggcgttat ttcttgatgt ctctgaccag
      541 acacccatca acagtattat tttctcccat gaagacggta cgcgactggg cgtggagcat
      601 ctggtcgcat tgggtcacca gcaaatcgcg ctgttagcgg gcccattaag ttctgtctcg
      661 gcgcgtctgc gtctggctgg ctggcataaa tatctcactc gcaatcaaat tcagccgata
      721 gcggaacggg aaggcgactg gagtgccatg tccggttttc aacaaaccat gcaaatgctg
      781 aatgagggca tcgttcccac tgcgatgctg gttgccaacg atcagatggc gctgggcgca
      841 atgcgcgcca ttaccgagtc cgggctgcgc gttggtgcgg atatctcggt agtgggatac
      901 gacgataccg aagacagctc atgttatatc ccgccgttaa ccaccatcaa acaggatttt
      961 cgcctgctgg ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg
     1021 aagggcaatc agctgttgcc cgtctcactg gtgaaaagaa aaaccaccct ggcgcccaat
     1081 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
     1141 tcccgactgg aaagcgggca gtaataatac tagagccagg catcaaataa aacgaaaggc
     1201 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta
     1261 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata
//
LOCUS       BBa_K934002             1304 bp    DNA     linear       17-May-2024
DEFINITION  LuxR/3OC6HSL complex dependent LacI generator
ACCESSION   BBa_K934002
VERSION     BBa_K934002.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=LuxR/HSL
     promoter        20..25
                     /label=-35
     promoter        42..47
                     /label=-10
     misc_feature    53..53
                     /label=start
     misc_feature    1..55
                     /label=BBa_R0062
     promoter        1..55
                     /label=BBa_R0062
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     CDS             82..1167
                     /label=BBa_I732100
     stem_loop       1187..1230
                     /label=stem_loop
     misc_feature    1176..1255
                     /label=BBa_B0010
     terminator      1176..1255
                     /label=BBa_B0010
     stem_loop       1271..1290
                     /label=T7 TE
     misc_feature    1297..1297
                     /label=stop
     polyA_site      1291..1304
                     /label=polya
     misc_feature    1264..1304
                     /label=BBa_B0012
     terminator      1264..1304
                     /label=BBa_B0012
     misc_feature    64..1304
                     /label=BBa_I732820
ORIGIN
        1 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga ataaatacta
       61 gagaaagagg agaaatacta gatgaaacca gtaacgttat acgatgtcgc agagtatgcc
      121 ggtgtctctt atcagaccgt ttcccgcgtg gtgaaccagg ccagccacgt ttctgcgaaa
      181 acgcgggaaa aagtggaagc ggcgatggcg gagctgaatt acattcccaa ccgcgtggca
      241 caacaactgg cgggcaaaca gtcgttgctg attggcgttg ccacctccag tctggccctg
      301 cacgcgccgt cgcaaattgt cgcggcgatt aaatctcgcg ccgatcaact gggtgccagc
      361 gtggtggtgt cgatggtaga acgaagcggc gtcgaagcct gtaaagcggc ggtgcacaat
      421 cttctcgcgc aacgcgtcag tgggctgatc attaactatc cgctggatga ccaggatgcc
      481 attgctgtgg aagctgcctg cactaatgtt ccggcgttat ttcttgatgt ctctgaccag
      541 acacccatca acagtattat tttctcccat gaagacggta cgcgactggg cgtggagcat
      601 ctggtcgcat tgggtcacca gcaaatcgcg ctgttagcgg gcccattaag ttctgtctcg
      661 gcgcgtctgc gtctggctgg ctggcataaa tatctcactc gcaatcaaat tcagccgata
      721 gcggaacggg aaggcgactg gagtgccatg tccggttttc aacaaaccat gcaaatgctg
      781 aatgagggca tcgttcccac tgcgatgctg gttgccaacg atcagatggc gctgggcgca
      841 atgcgcgcca ttaccgagtc cgggctgcgc gttggtgcgg atatctcggt agtgggatac
      901 gacgataccg aagacagctc atgttatatc ccgccgttaa ccaccatcaa acaggatttt
      961 cgcctgctgg ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg
     1021 aagggcaatc agctgttgcc cgtctcactg gtgaaaagaa aaaccaccct ggcgcccaat
     1081 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
     1141 tcccgactgg aaagcgggca gtaataatac tagagccagg catcaaataa aacgaaaggc
     1201 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta
     1261 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata
//