LOCUS       BBa_K934023             1804 bp    DNA     linear       03-May-2024
DEFINITION  3OC6HSL->3OC12HSL
ACCESSION   BBa_K934023
VERSION     BBa_K934023.1  
FEATURES             Location/Qualifiers
     CDS             85..687
                     /label=LasI
     misc_feature    688..720
                     /label=LVA
     misc_feature    727..751
                     /label=Help:Barcodes
     CDS             85..726
                     /label=BBa_C0078
     misc_feature    1..20
                     /label=LuxR/HSL
     promoter        20..25
                     /label=-35
     promoter        42..47
                     /label=-10
     misc_feature    53..53
                     /label=start
     misc_feature    1..55
                     /label=BBa_R0062
     promoter        1..55
                     /label=BBa_R0062
     RBS             64..78
                     /label=RBS-1\Strong
     RBS             71..75
                     /label=RBS
     misc_feature    64..78
                     /label=BBa_B0030
     RBS             64..78
                     /label=BBa_B0030
     CDS             85..687
                     /label=LasI
     misc_feature    688..720
                     /label=LVA
     misc_feature    727..751
                     /label=Help:Barcodes
     CDS             85..751
                     /label=BBa_C0078
     misc_feature    760..798
                     /label=B1006
     stem_loop       769..790
                     /label=modified thr terminator
     polyA_site      791..798
                     /label=PolyA
     polyA_site      760..768
                     /label=PolyA
     terminator      760..798
                     /label=BBa_B1006
     misc_feature    64..798
                     /label=BBa_K081016
     misc_feature    1..798
                     /label=BBa_K934022
     misc_feature    807..825
                     /label=TetR 1
     misc_feature    807..860
                     /label=BBa_R0040
     promoter        826..831
                     /label=-35
     misc_feature    832..850
                     /label=TetR 2
     promoter        849..854
                     /label=-10
     promoter        807..860
                     /label=BBa_R0040
     misc_feature    873..876
                     /label=conserved
     RBS             869..880
                     /label=BBa_B0034
     misc_feature    887..888
                     /label=prefix
     misc_feature    1060..1060
                     /label=T
     misc_feature    1378..1378
                     /label=A
     CDS             887..1636
                     /label=luxR
     misc_feature    887..1642
                     /label=BBa_C0062
     misc_feature    1643..1667
                     /label=Help:Barcodes
     CDS             887..1642
                     /label=BBa_C0062
     stem_loop       1687..1730
                     /label=stem_loop
     misc_feature    1676..1755
                     /label=BBa_B0010
     terminator      1676..1755
                     /label=BBa_B0010
     stem_loop       1771..1790
                     /label=T7 TE
     misc_feature    1797..1797
                     /label=stop
     polyA_site      1791..1804
                     /label=polya
     misc_feature    1764..1804
                     /label=BBa_B0012
     terminator      1764..1804
                     /label=BBa_B0012
     misc_feature    807..1804
                     /label=BBa_S03119
ORIGIN
        1 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga ataaatacta
       61 gagattaaag aggagaaata ctagatgatc gttcagatcg gtcgtcgtga agagttcgac
      121 aaaaaactgc tgggtgaaat gcacaaactg cgtgctcagg ttttcaaaga acgtaaaggt
      181 tgggacgttt ccgttatcga cgaaatggaa atcgacggtt acgacgctct gtccccgtac
      241 tacatgctga tccaggaaga caccccggaa gctcaggttt tcggttgctg gcgtatcttc
      301 gacaccaccg gtccgtacat gctgaaaaac accttcccgg aactgctgca cggtaaagaa
      361 gctccgtgct ccccgcacat ctgggaactg tcccgtttcg ctatcaactc cggtcagaaa
      421 ggttccctgg gtttctccga ctgcaccctg gaagctatgc gtgctctggc tcgttactcc
      481 ttgcagaacg acatccagac cctggttacc gttaccaccg ttggtgttga aaaaatgatg
      541 atccgtgctg gtctggacgt ttcccgtttc ggtccgcacc tgaaaatcgg tatcgaacgt
      601 gctgttgctc tgcgtatcga actgaacgct aaaacccaga tcgctctgta cggtggtgtt
      661 ctggttgaac agcgtctggc tgtttccgct gctaacgacg aaaactacgc tctggttgct
      721 taataactct gatagtgcta gtgtagatct ctactagaga aaaaaaaacc ccgcccctga
      781 cagggcgggg ttttttttta ctagagtccc tatcagtgat agagattgac atccctatca
      841 gtgatagaga tactgagcac tactagagaa agaggagaaa tactagatga aaaacataaa
      901 tgccgacgac acatacagaa taattaataa aattaaagct tgtagaagca ataatgatat
      961 taatcaatgc ttatctgata tgactaaaat ggtacattgt gaatattatt tactcgcgat
     1021 catttatcct cattctatgg ttaaatctga tatttcaatc ctagataatt accctaaaaa
     1081 atggaggcaa tattatgatg acgctaattt aataaaatat gatcctatag tagattattc
     1141 taactccaat cattcaccaa ttaattggaa tatatttgaa aacaatgctg taaataaaaa
     1201 atctccaaat gtaattaaag aagcgaaaac atcaggtctt atcactgggt ttagtttccc
     1261 tattcatacg gctaacaatg gcttcggaat gcttagtttt gcacattcag aaaaagacaa
     1321 ctatatagat agtttatttt tacatgcgtg tatgaacata ccattaattg ttccttctct
     1381 agttgataat tatcgaaaaa taaatatagc aaataataaa tcaaacaacg atttaaccaa
     1441 aagagaaaaa gaatgtttag cgtgggcatg cgaaggaaaa agctcttggg atatttcaaa
     1501 aatattaggt tgcagtgagc gtactgtcac tttccattta accaatgcgc aaatgaaact
     1561 caatacaaca aaccgctgcc aaagtatttc taaagcaatt ttaacaggag caattgattg
     1621 cccatacttt aaaaattaat aacactgata gtgctagtgt agatcactac tagagccagg
     1681 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg
     1741 tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt
     1801 tata
//