LOCUS       BBa_K934025              959 bp    DNA     linear       01-Jun-2024
DEFINITION  Plux/tet-GFP
ACCESSION   BBa_K934025
VERSION     BBa_K934025.1  
FEATURES             Location/Qualifiers
     promoter        42..47
                     /label=-10
     misc_feature    2..20
                     /label=LuxR
     promoter        21..26
                     /label=-35
     misc_feature    25..45
                     /label=Otet
     misc_feature    55..72
                     /label=Otet
     promoter        1..76
                     /label=BBa_K934024
     misc_feature    89..92
                     /label=conserved
     RBS             85..96
                     /label=BBa_B0034
     CDS             103..822
                     /label=GFP protein
     CDS             103..822
                     /label=BBa_E0040
     stem_loop       842..885
                     /label=stem_loop
     misc_feature    831..910
                     /label=BBa_B0010
     terminator      831..910
                     /label=BBa_B0010
     stem_loop       926..945
                     /label=T7 TE
     misc_feature    952..952
                     /label=stop
     polyA_site      946..959
                     /label=polya
     misc_feature    919..959
                     /label=BBa_B0012
     terminator      919..959
                     /label=BBa_B0012
     misc_feature    85..959
                     /label=BBa_I13504
ORIGIN
        1 acctgtagga tcgtacaggt ttactcccta tcagtgatag agatactcgt attatcccta
       61 tcagtgatag agattatact agagaaagag gagaaatact agatgcgtaa aggagaagaa
      121 cttttcactg gagttgtccc aattcttgtt gaattagatg gtgatgttaa tgggcacaaa
      181 ttttctgtca gtggagaggg tgaaggtgat gcaacatacg gaaaacttac ccttaaattt
      241 atttgcacta ctggaaaact acctgttcca tggccaacac ttgtcactac tttcggttat
      301 ggtgttcaat gctttgcgag atacccagat catatgaaac agcatgactt tttcaagagt
      361 gccatgcccg aaggttatgt acaggaaaga actatatttt tcaaagatga cgggaactac
      421 aagacacgtg ctgaagtcaa gtttgaaggt gatacccttg ttaatagaat cgagttaaaa
      481 ggtattgatt ttaaagaaga tggaaacatt cttggacaca aattggaata caactataac
      541 tcacacaatg tatacatcat ggcagacaaa caaaagaatg gaatcaaagt taacttcaaa
      601 attagacaca acattgaaga tggaagcgtt caactagcag accattatca acaaaatact
      661 ccaattggcg atggccctgt ccttttacca gacaaccatt acctgtccac acaatctgcc
      721 ctttcgaaag atcccaacga aaagagagac cacatggtcc ttcttgagtt tgtaacagct
      781 gctgggatta cacatggcat ggatgaacta tacaaataat aatactagag ccaggcatca
      841 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt
      901 gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct gcgtttata
//