LOCUS       BBa_K952006             3360 bp    DNA     linear       27-May-2024
DEFINITION  const. promoter-B0034-YF1-B0034-FixJ-PfixK2-Lysis Cassette
ACCESSION   BBa_K952006
VERSION     BBa_K952006.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23102
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             62..1192
                     /label=YF1
     CDS             62..1192
                     /label=BBa_K592004
     misc_feature    1205..1208
                     /label=conserved
     RBS             1201..1212
                     /label=BBa_B0034
     CDS             1219..1839
                     /label=FixJ
     CDS             1219..1839
                     /label=BBa_K592005
     misc_feature    44..1839
                     /label=BBa_K592016
     promoter        1848..2097
                     /label=FixK2 promoter
     promoter        1848..2097
                     /label=BBa_K592006
     CDS             2104..3360
                     /label=BBa_K124017
     misc_feature    44..3360
                     /label=BBa_K952002
ORIGIN
        1 ttgacagcta gctcagtcct aggtactgtg ctagctacta gagaaagagg agaaatacta
       61 gatggctagt tttcaatcat ttgggatacc aggacagctg gaagtcatca aaaaagcact
      121 tgatcacgtg cgagtcggtg tggtaattac agatcccgca cttgaagata atcctattgt
      181 ctacgtaaat caaggctttg ttcaaatgac cggctacgag accgaggaaa ttttaggaaa
      241 gaactgtcgc ttcttacagg ggaaacacac agatccggca gaagtggaca acatcagaac
      301 cgctttacaa aataaagaac cggtcaccgt tcagatccaa aactacaaaa aagacggaac
      361 gatgttctgg aatgaattaa atattgatcc aatggaaata gaggataaaa cgtattttgt
      421 cggcattcag aatgatatca ccgagcacca gcagacccag gcgcgtctcc aggaactgca
      481 atccgagctc gtccacgtct ccaggctgag cgccatgggc gaaatggcgt ccgcgctcgc
      541 gcacgagctc aaccagccgc tggcggcgat cagcaactac atgaagggct cgcggcggct
      601 gcttgccggc agcagtgatc cgaacacacc gaaggtcgaa agcgccctgg accgcgccgc
      661 cgagcaggcg ctgcgcgccg gccagatcat ccggcgcctg cgcgacttcg ttgcccgcgg
      721 cgaatcggag aagcgggtcg agagtctctc caagctgatc gaggaggccg gcgcgctcgg
      781 gcttgccggc gcgcgcgagc agaacgtgca gctccgcttc agtctcgatc cgggcgccga
      841 tctcgttctc gccgaccggg tgcagatcca gcaggtcctg gtcaacctgt tccgcaacgc
      901 gctggaagcg atggctcagt cgcagcgacg cgagctcgtc gtcaccaaca cccccgccgc
      961 cgacgacatg atcgaggtcg aagtgtccga caccggcagc ggtttccagg acgacgtcat
     1021 tccgaacctg tttcagactt tcttcaccac caaggacacc ggcatgggcg tgggactgtc
     1081 catcagccgc tcgatcatcg aagctcacgg cgggcgcatg tgggccgaga gcaacgcatc
     1141 gggcggggcg accttccgct tcaccctccc ggcagccgac gagaattaat aatactagag
     1201 aaagaggaga aatactagat gacgaccaag ggacatatct acgtcatcga cgacgacgcg
     1261 gcgatgcggg attcgctgaa tttcctgctg gattctgccg gcttcggcgt cacgctgttt
     1321 gacgacgcgc aagcctttct cgacgccctg ccgggtctct ccttcggctg cgtcgtctcc
     1381 gacgtgcgca tgccgggcct tgacggcatc gagctgttga agcggatgaa ggcgcagcaa
     1441 agcccctttc cgatcctcat catgaccggt cacggcgacg tgccgctcgc ggtcgaggcg
     1501 atgaagttag gggcggtcga ctttctggaa aagcctttcg aggacgaccg cctcaccgcc
     1561 atgatcgaat cggcgatccg ccaggccgag ccggccgcca agagcgaggc cgtcgcgcag
     1621 gatatcgccg cccgcgtcgc ctcgttgagc cccagggagc gccaggtcat ggaagggctg
     1681 atcgccggcc tttccaacaa gctgatcgcc cgcgagtacg acatcagccc gcgcaccatc
     1741 gaggtgtatc gggccaacgt catgaccaag atgcaggcca acagcctttc ggagctggtt
     1801 cgcctcgcga tgcgcgccgg catgctcaac gattaataat actagaggcc ggagccgatt
     1861 atccgcaccc gtccttggtc ttggacacac tcgctcgcga tccgaagccg cctttcaaag
     1921 atactccgca gtgaaacgca tcttttaagc gcgacttgtc gccttcgcgc agcagaacac
     1981 tcgtatggca ctcaatccat gatcgcttcg ttcgctccga cgcgcgttgc ggggcccccc
     2041 tcgaccggat gacaacacat tgcgtcacct caactgctgc gcgcgcactg cgtcacatac
     2101 tagatgccag aaaaacatga cctgttggcc gccattctcg cggcaaagga acaaggcatc
     2161 ggggcaatcc ttgcgtttgc aatggcgtac cttcgcggca gatataatgg cggtgcgttt
     2221 acaaaaacag taatcgacgc aacgatgtgc gccattatcg cctggttcat tcgtgacctt
     2281 ctcgacttcg ccggactaag tagcaatctc gcttatataa cgagcgtgtt tatcggctac
     2341 atcggtactg actcgattgg ttcgcttatc aaacgcttcg ctgctaaaaa agccggagta
     2401 gaagatggta gaaatcaata aatggtagaa atcaataatc aacgtaaggc gttcctcgat
     2461 atgctggcgt ggtcggaggg aactgataac ggacgtcaga aaaccagaaa tcatggttat
     2521 gacgtcattg taggcggaga gctatttact gattactccg atcaccctcg caaacttgtc
     2581 acgctaaacc caaaactcaa atcaacaggc gccggacgct accagcttct ttcccgttgg
     2641 tgggatgcct accgcaagca gcttggcctg aaagacttct ctccgaaaag tcaggacgct
     2701 gtggcattgc agcagattaa ggagcgtggc gctttaccta tgattgatcg tggtgatatc
     2761 cgtcaggcaa tcgaccgttg cagcaatatc tgggcttcac tgccgggcgc tggttatggt
     2821 cagttcgagc ataaggctga cagcctgatt gcaaaattca aagaagcggg cggaacggtc
     2881 agagagattg atgtatgaat gagcagagtc accgcgatta tctccgctct ggttatctgc
     2941 atcatcgtct gcctgtcatg ggctgttaat cattaccgtg ataacgccat tacctacaaa
     3001 gcccagcgcg acaaaaatgc cagagaactg aagctggcga acgcggcaat tactgacatg
     3061 cagatgcgtc agcgtgatgt tgctgcgctc gatgcaaaat acacgaagga gttagctgat
     3121 gctaaagctg aaaatgatgc tctgcgtgat gatgttgccg ctggtcgtcg tcggttgcac
     3181 atcaaagcag tctgtcagtc agtgcgtgaa gccaccaccg cctccggcgt ggataatgca
     3241 gcctcccccc gactggcaga caccgctgaa cgggattatt tcaccctcag agagaggctg
     3301 atcactatgc aaaaacaact ggaaggaacc cagaagtata ttaatgagca gtgcagatag
//