BBa_K953002 1 BBa_K953002 Agrobacterium tumefaciens Bacteriophytochrome with RBS (E. coli Codon Optimised) 2012-09-23T11:00:00Z 2015-05-08T01:13:49Z Agrobacterium tumefaciens Released HQ 2013 Agrobacterium tumefaciens bacteriophytochrome, codon optimised for expression in E. coli. In the presence of biliverdin the bacteriophytochrome will undergo a conformation change. This complex can then absorb red light(620-750 nm) to excite the complex and result in a phenotypic change from blue to green. This can be reversed by far-red light (700-800 nm) or will revert from green to blue over time. As E. coli does not produce biliverdin, heme oxygenase (Part BBa_K953000) can be coupled with this part to activate the oxidation of heme to produce biliverdin or by the addition of biliverdin to the cells. false false _1221_ 0 14275 9 In stock true Optimised for expression in E. coli false Daniel Winter annotation2196369 1 Start Codon range2196369 1 23 25 annotation2196372 1 BBa_K953002 range2196372 1 1 2236 annotation2196368 1 RBS range2196368 1 1 22 annotation2196371 1 Stop range2196371 1 2231 2236 annotation2196370 1 Bacteriophytochrome range2196370 1 26 2230 BBa_K953002_sequence 1 ctttaagaaggagatatacataatgagttcacatacgccgaaactggatagttgcggcgcagagcccatccacattcccggtgctattcaggaacatggagcccttctggttctgtccgcgcgggaattttccgtggtgcaggcgagcgataatctcgccaactatattggtgtggacctgccaatcggggccgttgccaccgaagcaaacttgccgttcatctccgtactgtccgcctggtattccggcgaggagagcaatttccgatatgcctgggccgaaaagaaactcgatgtttcggcgcatcgatccgggaccttggttattcttgaggtggaaaaggcaggagtgggcgaatccgctgaaaagctgatgggcgaactcaccagtctggcgaaatatctgaacagtgcgccgtctctggaggacgcgctttttcggacagcgcaactggtgtcttctatcagcggtcatgaccgtacactgatctatgatttcggactggactggtctggtcatgttgtggcggaagcgggatctggtgctttgccgtcttatctcggtcttcgctttcctgcgggtgacatccctccgcaagcgcggcaactctatacaatcaatcgcctgcggatgattcctgatgttgattataagcctgttccgatcagaccggaggttaatgcggaaacaggagcggttctcgatatgagcttttctcaacttcgctctgtatctccggttcatctcgaatatatgcgcaatatgggtactgccgcttctatgtctgttagcatcgttgttaatggtgctctctggggcttgattgcctgtcaccacgcgacacctcattctgtatcattagcagttcgtgaagcctgtgattttgcagcgcagctcctgtcgatgcggatcgcgatggagcagagttcccaggatgcatcccgccgggtggagcttggccacattcaggcgcggctcttgaaaggcatggccgccgccgaaaaatgggttgatggcctgctgggaggcgaaggtgaaagagaagacctgctgaaacaggtgggcgcggatggcgcagcacttgtcctgggcgacgactatgagctggtcggcaacacgccctcacgcgagcaggttgaagaattgattctgtggcttggcgagcgggagatcgccgatgtcttcgcgaccgacaatcttgctggtaactaccccaccgccgcggcttatgcttccgttgcgagcggcattatcgcgatgcgggtttcggagttgcacggcagctggctcatctggtttcggccggaagtcataaaaacagttcgttggggtggtgatcctcacaagacggttcaggagtctggtcgtattcatcctcgtaagtcctttgaaatctggaaggaacaattgcgtaacacctcctttccttggtcagagcctgaacttgcagcagcgcgtgaacttcgtggtgcaatcatcggtattgtcctgcgtaagacagaggaaatggcagatctgacacgtgagttgcagcgtaccaataaagagctggaagctttttcctattccgtttctcatgatctgcgtgctccgttccgtcatattgtcgggtttgcacagttgttgcgtgagcgttccgatgccctggatgagaagtccctgcattatctacagatgatatcagaggcagcgctgggtgcaggcaggttggtggatgatctcctgaatttttcacagcttggtcgaacgcaactcaccctgaaacctgttgatatgcaaaaagttgtgtctgaagttcgtcgttccctgtcacacgcggtttccgatcgccagatcgagtggcgcatcggagcgttgccggtgatattcggggatccgaccctgctgcggcaggtctggtacaatctcattgaaaacgccatcaaatattcgtcccgggagccggtgtcgatcatcaccatttctgcggttgaaaccgaggatgacgtcacttattcggtcgaagacaatggtgtcggctttgacatggcctattacaacaaactttttggcgtgtttcagaggctacagcgggtggaggatttcgagggaactggcattggacttgcgctggtgcgacgaattgtcgaacgccatcacggtttggttggtgccgagggaactgtcggtgagggagccacgttctccttcacattgccggttacgaaagtggaagaagaaaaaattgcgtaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z