LOCUS       BBa_K957000             2625 bp    DNA     linear       12-May-2024
DEFINITION  Arsenic inducible mtrB with cut sites flanking RBS
ACCESSION   BBa_K957000
VERSION     BBa_K957000.1  
FEATURES             Location/Qualifiers
     misc_feature    1..472
                     /label=BBa_J33201
     misc_feature    36..54
                     /label=ArsR binding site
     promoter        57..62
                     /label=-35 sequence
     promoter        80..85
                     /label=-10 sequence
     RBS             108..112
                     /label=rbs
     CDS             119..472
                     /label=arsR
     misc_feature    474..481
                     /label=AscI cut site
     RBS             484..489
                     /label=mtrB rbs
     misc_feature    491..496
                     /label=BamHI cut site
     CDS             497..2590
                     /label=mtrB
     misc_feature    497..2590
                     /label=BBa_K098989
     misc_feature    2620..2625
                     /label=KpnI cut site
ORIGIN
        1 ccaactcaaa attcacacct attaccttcc tctgcactta cacattcgtt aagtcatata
       61 tgtttttgac ttatccgctt cgaagagaga cactacctgc aacaatcagg agcgcaatat
      121 gtcatttctg ttacccatcc aattgttcaa aattcttgct gatgaaaccc gtctgggcat
      181 cgttttactg ctcagcgaac tgggagagtt atgcgtctgc gatctctgca ctgctctcga
      241 ccagtcgcag cccaagatct cccgccacct ggcattgctg cgtgaaagcg ggctattgct
      301 ggaccgcaag caaggtaagt gggttcatta ccgcttatca ccgcatattc cagcatgggc
      361 ggcgaaaatt attgatgagg cctggcgatg tgaacaggaa aaggttcagg cgattgtccg
      421 caacctggct cgacaaaact gttccgggga cagtaagaac atttgcagtt aacggcgcgc
      481 ctaaggagac ggatccatga aatttaaact caatttgatc actctagcgt tattagccaa
      541 cacaggcttg gccgtcgctg ctgatggtta tggtctagcg aatgccaata ctgaaaaagt
      601 gaaattatcc gcatggagct gtaaaggctg cgtcgttgaa acgggcacat caggcactgt
      661 gggtgtcggt gtcggttata acagcgaaga ggatattcgc tctgccaatg cctttggtac
      721 atccaatgaa gtggcgggta aatttgatgc cgatttaaac tttaaaggtg aaaagggtta
      781 tcgtgccagt gttgatgctt atcaactcgg tatggatggc ggtcgcttag atgtcaatgc
      841 gggcaaacaa ggccagtaca acgtcaatgt gaactatcgc caaattgcta cctacgacag
      901 caatagcgcc ctatcgccct acgcgggtat tggtggcaat aacctcacgt taccggataa
      961 ctggataaca gcaggttcaa gcaaccaaat gccactcttg atggacagcc tcaatgccct
     1021 cgaactctca cttaaacgtg agcgcacggg gttgggattt gaatatcaag gtgaatccct
     1081 gtggagcacc tatgttaact acatgcgtga agagaaaacc ggcttaaaac aagcctctgg
     1141 tagcttcttc aaccaatcga tgatgttagc agagccggtg gattacacca ctgacaccat
     1201 tgaagcgggt gtcaaactca agggtgatcg ttggtttacc gcactcagtt acaatgggtc
     1261 aatattcaaa aacgaataca accaattgga ctttgaaaat gcttttaacc ccacctttgg
     1321 tgctcaaacc caaggtacga tggcactcga tccggataac cagtcacaca ccgtgtcgct
     1381 gatgggacag tacaacgatg gcagcaacgc actgtcgggt cgtattctga ccggacaaat
     1441 gagccaagat caggcgttag tgacggataa ctaccgttat gctaatcagc tcaataccga
     1501 tgccgtcgat gccaaagtcg atctactggg tatgaacctg aaagtcgtta gcaaagtgag
     1561 caatgatctt cgcttaacag gtagttacga ttattacgac cgtgacaata atacccaagt
     1621 agaagaatgg actcagatca gcatcaacaa tgtcaacggt aaggtggctt ataacacccc
     1681 ttacgataat cgtacgcaac gctttaaagt tgccgcagat tatcgcatta cccgcgatat
     1741 caaactcgat ggtggttatg acttcaaacg tgaccaacgt gattatcaag accgtgaaac
     1801 cacggatgaa aataccgttt gggcccgttt acgtgtaaac agcttcgata cttgggacat
     1861 gtgggtaaaa ggcagttacg gtaaccgtga cggctcacaa taccaagcgt ctgaatggac
     1921 ctcttctgaa accaacagcc tgttacgtaa gtacaatctg gctgaccgtg acagaactca
     1981 agtcgaagca cggatcaccc attcgccatt agaaagcctg actatcgatg ttggtgcccg
     2041 ttacgcgtta gatgattata ccgatactgt gattggatta actgagtcaa aagacaccag
     2101 ttatgatgcc aacatcagtt atatgatcac cgctgactta ctggcaaccg ccttctacaa
     2161 ttaccaaacc attgagtctg aacaggcggg tagcagcaat tacagcaccc caacgtggac
     2221 aggctttata gaagatcagg tagatgtggt cggtgcaggt atcagctaca acaatctgct
     2281 ggagaacaag ttacgcctag gactggacta cacctattcc aactccgaca gtaacactca
     2341 agtcagacaa ggtatcactg gcgactatgg tgattatttt gccaaagtgc ataacattaa
     2401 cttatacgct caatatcaag ccaccgagaa actcgcgctg cgcttcgatt acaaaattga
     2461 gaactataag gacaatgacg ccgcaaatga tatcgccgtt gatggcattt ggaacgtcgt
     2521 aggttttggt agtaacagcc atgactacac cgcacaaatg ctgatgctga gcatgagtta
     2581 caaactctaa tactagagtc acactggctc accttctatg gtacc
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