BBa_K957000 1 BBa_K957000 Arsenic inducible mtrB with cut sites flanking RBS 2012-07-25T11:00:00Z 2015-05-08T01:13:49Z Arsenic-sensitive component of this composite part was originally derived from the arsenic detoxification operon of E. coli strain JM109; BioBrick part BBa_J33201 was the source of DNA. mtrB is originally derived from the mtrCAB operon of Shewanella oneidensis MR-1; BioBrick part BBa_K098994 was used as DNA source. The purpose of this part is to upregulate mtrB expression in response to arsenic. When expressed in a Shewanella strain lacking mtrB on the chromosome, this composite part will function as a component of a biosensor. Specifically, when such a complemented strain is inoculated in a microbial electrochemical system, current output will increase in response to arsenic. Within the genetic circuit, arsR acts as a negative autoregulator, repressing not only the expression of downstream mtrB, but also its own expression. Upon association with arsenic salts, ArsR dissociates from the operator of the arsenic inducible promoter, upregulating expression of downstream protein. Because mtrB activity is requisite for functionality of the mtr electron pathway in Shewanella spp., arsenic thus induces electron shuttling through the mtr pathway. false false _1228_ 0 13504 9 It's complicated true Primers were designed such that the wild-type Shine Dalgarno sequence was included upstream of the mtrB start codon, maintaining spacing. However, the appended BamHI cutsite downstream of the Shine Dalgarno sequence may alter translational efficiency. false Dylan Patrick Webster annotation2178744 1 BBa_K098989 range2178744 1 497 2590 annotation2179155 1 KpnI cut site range2179155 1 2620 2625 annotation2178743 1 mtrB range2178743 1 497 2590 annotation2178737 1 -10 sequence range2178737 1 80 85 annotation2178734 1 BBa_J33201 range2178734 1 1 472 annotation2178738 1 rbs range2178738 1 108 112 annotation2178742 1 BamHI cut site range2178742 1 491 496 annotation2178740 1 AscI cut site range2178740 1 474 481 annotation2178735 1 ArsR binding site range2178735 1 36 54 annotation2178739 1 arsR range2178739 1 119 472 annotation2178736 1 -35 sequence range2178736 1 57 62 annotation2178741 1 mtrB rbs range2178741 1 484 489 BBa_K957000_sequence 1 ccaactcaaaattcacacctattaccttcctctgcacttacacattcgttaagtcatatatgtttttgacttatccgcttcgaagagagacactacctgcaacaatcaggagcgcaatatgtcatttctgttacccatccaattgttcaaaattcttgctgatgaaacccgtctgggcatcgttttactgctcagcgaactgggagagttatgcgtctgcgatctctgcactgctctcgaccagtcgcagcccaagatctcccgccacctggcattgctgcgtgaaagcgggctattgctggaccgcaagcaaggtaagtgggttcattaccgcttatcaccgcatattccagcatgggcggcgaaaattattgatgaggcctggcgatgtgaacaggaaaaggttcaggcgattgtccgcaacctggctcgacaaaactgttccggggacagtaagaacatttgcagttaacggcgcgcctaaggagacggatccatgaaatttaaactcaatttgatcactctagcgttattagccaacacaggcttggccgtcgctgctgatggttatggtctagcgaatgccaatactgaaaaagtgaaattatccgcatggagctgtaaaggctgcgtcgttgaaacgggcacatcaggcactgtgggtgtcggtgtcggttataacagcgaagaggatattcgctctgccaatgcctttggtacatccaatgaagtggcgggtaaatttgatgccgatttaaactttaaaggtgaaaagggttatcgtgccagtgttgatgcttatcaactcggtatggatggcggtcgcttagatgtcaatgcgggcaaacaaggccagtacaacgtcaatgtgaactatcgccaaattgctacctacgacagcaatagcgccctatcgccctacgcgggtattggtggcaataacctcacgttaccggataactggataacagcaggttcaagcaaccaaatgccactcttgatggacagcctcaatgccctcgaactctcacttaaacgtgagcgcacggggttgggatttgaatatcaaggtgaatccctgtggagcacctatgttaactacatgcgtgaagagaaaaccggcttaaaacaagcctctggtagcttcttcaaccaatcgatgatgttagcagagccggtggattacaccactgacaccattgaagcgggtgtcaaactcaagggtgatcgttggtttaccgcactcagttacaatgggtcaatattcaaaaacgaatacaaccaattggactttgaaaatgcttttaaccccacctttggtgctcaaacccaaggtacgatggcactcgatccggataaccagtcacacaccgtgtcgctgatgggacagtacaacgatggcagcaacgcactgtcgggtcgtattctgaccggacaaatgagccaagatcaggcgttagtgacggataactaccgttatgctaatcagctcaataccgatgccgtcgatgccaaagtcgatctactgggtatgaacctgaaagtcgttagcaaagtgagcaatgatcttcgcttaacaggtagttacgattattacgaccgtgacaataatacccaagtagaagaatggactcagatcagcatcaacaatgtcaacggtaaggtggcttataacaccccttacgataatcgtacgcaacgctttaaagttgccgcagattatcgcattacccgcgatatcaaactcgatggtggttatgacttcaaacgtgaccaacgtgattatcaagaccgtgaaaccacggatgaaaataccgtttgggcccgtttacgtgtaaacagcttcgatacttgggacatgtgggtaaaaggcagttacggtaaccgtgacggctcacaataccaagcgtctgaatggacctcttctgaaaccaacagcctgttacgtaagtacaatctggctgaccgtgacagaactcaagtcgaagcacggatcacccattcgccattagaaagcctgactatcgatgttggtgcccgttacgcgttagatgattataccgatactgtgattggattaactgagtcaaaagacaccagttatgatgccaacatcagttatatgatcaccgctgacttactggcaaccgccttctacaattaccaaaccattgagtctgaacaggcgggtagcagcaattacagcaccccaacgtggacaggctttatagaagatcaggtagatgtggtcggtgcaggtatcagctacaacaatctgctggagaacaagttacgcctaggactggactacacctattccaactccgacagtaacactcaagtcagacaaggtatcactggcgactatggtgattattttgccaaagtgcataacattaacttatacgctcaatatcaagccaccgagaaactcgcgctgcgcttcgattacaaaattgagaactataaggacaatgacgccgcaaatgatatcgccgttgatggcatttggaacgtcgtaggttttggtagtaacagccatgactacaccgcacaaatgctgatgctgagcatgagttacaaactctaatactagagtcacactggctcaccttctatggtacc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z