Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: FURKAN SACİT CEYLAN
Date created: 2013-06-13 11:00:00
Date modified: 2015-05-08 01:13:51
Urease generator with ROSE thermometer
| Types | DnaRegion |
| Roles | Translational_Unit engineered_region |
| Sequences | BBa_K993003_sequence (Version 1) |
Description
Repression of heat shock gene expression (ROSE) elementThe repression of heat shock gene expression (ROSE) element is an RNA element found in the 5' UTR of some heat shock protein's mRNAs. The ROSE element is an RNA thermometer that negatively regulates heat shock gene expression. The secondary structure is thought to be altered by temperature, thus it is an RNA thermometer. This structure blocks access to the ribosome binding site at normal temperatures. During heat shock however, the structure changes freeing the ribosome binding site and allowing expression to occur.[2][3]
A partial structure of this RNA element has been determined using NMR.[1]
ROSE1 and ROSEAT2 are specific examples of ROSE elements.[4] ROSE1 is found in Bradyrhizobium japonicum whereas ROSEAT2 is a closely related element from Agrobacterium tumefaciens. The two RNA elements have similar secondary structures with ROSE1 having an extra hairpin.[4] All ROSE elements contain a characteristic 'bulged G' opposite the Shine-Dalgarno sequence binding site, without this nucleotide the RNA thermometer loses its temperature-sensitivity.[5]
Notes
Note that RNA thermometer actually works as an RBS sequence. Therefore, this part does not include an additional ribosome binding site. Also, this part does not contain an terminator sequence, but a simple stop codon. The RFP should be translated continuously.Source
AATTTTGTCAAAATAATTTTATTGACAACGTCTTATTAACGTTGATATAATTTAAATTTTTTTTGACAAAAATGGGCTCGTGTTGTACAATAAATGTGCCGCGACAAGCGGTCCGGGCGCCCTAGGGGCCCGGCGGAGACGGGCGCCGGAGGTGTCCGACGCCTGCTCGTCAAGTTCTTGCTCCTTGGAGGATATGAAGATAAACAGGCAGCAGTACGCGGAGAGCTACGGGCCGACGGTGGGGGACAGGGTGAGGCTGGCGGACACGGACCTGGGGGAGGTGGAGAAGGACTACTACTACCTGGGGGACGAGGTGAACTTCGGGGGGGGGAAGGTGCTGAGGGAGGGGATGGGGGAGAACGGGACGTACACGAGGACGGAGAACGTGTTGGACCTGCTGCTGACGAACGCGCTGATATTGGACTACACGGGGATATACAAGGCGGACATAGGGGTGAAGGACGGGTACATAGTGGGGATAGGGAAGGGGGGGAACCCGGACATAATGGACGGGGTGACGCCGAACATGATAGTGGGGACGGCGACGGAGGTGATAGCGGCGGAGGGGAAGATAGTGACGGCGGGGGGGATAGACACGCACGTGCACTTCATAAACCCGGACCAGGTGGACGTGGCGCTGGCGAACGGGATAACGACGCTGTTCGGGGGGGGGACGGGGCCGGCGGAGGGGAGCAAGGCGACGACGGTGACGCCGGGGCCGTGGAACATAGAGAAGATGCTGAAGAGCACGGAGGGGCTGCCGATAAACGTGGGGATACTGGGGAAGGGGCACGGGAGCAGCATAGCGCCGATAATGGAGCAGATAGACGCGGGGGCGGCGGGGCTGAAGATACACGAGGACTGGGGGGCGACGCCGGCGAGCATAGACAGGAGCCTGACGGTGGCGGACGAGGCGGACGTGCAGGTGGCGATACACAGCGACACGCTGAACGAGGCGGGGTTCTTGGAGGACACGGTGAGGGCGATAAACGGGAGGGTGATACACAGCTTCCACGTGGAGGGGGCGGGGGGGGGGCACGCGCCGGACATAATGGCGATGGCGGGGCACCCGAACGTGCTGCCGAGCAGCACGAACCCGACGAGGCCGTTCACGGTGAACACGATAGACGAGCACTTGGACATGCTGATGGTGTGCCACCACCTGAAGCAGAACATACCGGAGGACGTGGCGTTCGCGGACAGCAGGATAAGGCCGGAGACGATAGCGGCGGAGGACATACTGCACGACCTGGGGATAATAAGCATGATGAGCACGGACGCGCTGGCGATGGGGAGGGCGGGGGAGATGGTGCTGAGGACGTGGCAGACGGCGGACAAGATGAAGAAGCAGAGGGGGCCGCTGGCGGAGGAGAAGAACGGGAGCGACAACTTCAGGCTGAAGAGGTACGTGAGCAAGTACACGATAAACCCGGCGATAGCGCAGGGGATGGCGCACGAGGTGGGGAGCATAGAGGAGGGGAAGTTCGCGGACCTGGTGCTGTGGGAGCCGAAGTTCTTCGGGGTGAAGGCGGACAGGGTGATAAAGGGGGGGATAATAGCGTACGCGCAGATAGGGGACCCGAGCGCGAGCATACCGACGCCGCAGCCGGTGATGGGGAGGAGGATGTACGGGACGGTGGGGGACCTGATACACGACACGAACATAACGTTCATGAGCAAGAGCAGCATACAGCAGGGGGTGCCGGCGAAGCTGGGGCTGAAGAGGAGGATAGGGACGGTGAAGAATTGTCGAAACATAGGGAAGAAGGACATGAAGTGGAACGACGTGACGACGGACATAGACATAAACCCGGAGACGTACGAGGTGAAGGTGGACGGGGAGGTGCTGACGTGCGAGCCGGTGAAGGAGCTGCCGATGGCGCAGAGGTACTTCCTGTTCTAATCGCTGGGACGCCCGCCCCTAGAAAAGGAGGACTAAAAATGAGCAACAACAACTACATAGTGCCGGGGGAGTACAGGGTGGCGGAGGGGGAGATAGAGATAAACGCGGGGAGGGAGAAGACGACGATAAGGGTGAGCAACACGGGGGACAGGCCGATACAGGTGGGGAGCCACATACACTTCGTGGAGGTGAACAAGGAGCTGCTGTTCGACAGGGCGGAGGGGATAGGGAGGAGGCTGAACATACCGAGCGGGACGGCGGCGAGGTTCGAGCCGGGGGAGGAGATGGAGGTGGAGCTGACGGAGCTGGGGGGGAACAGGGAGGTGTTCGGGATAAGCGACCTGACGAACGGGAGCGTGGACAACAAGGAGCTGATATTACAAAGGGCGAAGGAGCTGGGGTACAAGGGGGTGGAGTAATCGCTGGGACGCCCGCCCCTAGAAAAGGAGGACTAAAAATGCATCTTAATCCCGCCGAAAAAGAAAAATTACAAATCTTTCTTGCCTCAGAACTTCTTCTTCGACGAAAAGCCCGAGGCCTTAAACTTAATTATCCCGAAGCCGTCGCCATCATCACCTCATTTATCATGGAAGGCGCCCGAGATGGCAAAACCGTCGCCATGCTTATGGAAGAAGGCAAACATGTCCTTACCCGAGATGATGTCATGGAAGGCGTCCCCGAAATGATCGATGATATCCAAGCCGAAGCCACCTTTCCCGATGGCACCAAGTTGGTCACCGTCCATAATCCCATCTCATAA| Sequence Annotation | Location | Component / Role(s) |
| pVeg2 ROSE Thermometer Urease A Insulator RBS Start Codon Urease B Insulator RBS Urease C Start Codon Start Codon Stop Codon Stop Codon Stop Codon | 1,97 98,193 194,1903 1904,1918 1919,1941 194,196 1942,2322 2323,2337 2338,2360 2361,2663 1942,1944 2361,2363 1901,1903 2320,2322 2661,2663 | promoter feature/promoter feature/rbs ribosome_entry_site CDS feature/protein feature/dna sequence_feature feature/rbs ribosome_entry_site start_codon feature/start feature/protein CDS sequence_feature feature/dna ribosome_entry_site feature/rbs CDS feature/protein feature/start start_codon feature/start start_codon feature/stop stop_codon stop_codon feature/stop feature/stop stop_codon |