LOCUS       BBa_K993003             2663 bp    DNA     linear       06-May-2024
DEFINITION  Urease generator with ROSE thermometer
ACCESSION   BBa_K993003
VERSION     BBa_K993003.1  
FEATURES             Location/Qualifiers
     promoter        1..97
                     /label=pVeg2
     RBS             98..193
                     /label=ROSE Thermometer 
     CDS             194..1903
                     /label=Urease A
     misc_feature    1904..1918
                     /label=Insulator
     RBS             1919..1941
                     /label=RBS
     misc_feature    194..196
                     /label=Start Codon
     CDS             1942..2322
                     /label=Urease B
     misc_feature    2323..2337
                     /label=Insulator
     RBS             2338..2360
                     /label=RBS
     CDS             2361..2663
                     /label=Urease C
     misc_feature    1942..1944
                     /label=Start Codon
     misc_feature    2361..2363
                     /label=Start Codon
     misc_feature    1901..1903
                     /label=Stop Codon
     misc_feature    2320..2322
                     /label=Stop Codon
     misc_feature    2661..2663
                     /label=Stop Codon
ORIGIN
        1 aattttgtca aaataatttt attgacaacg tcttattaac gttgatataa tttaaatttt
       61 ttttgacaaa aatgggctcg tgttgtacaa taaatgtgcc gcgacaagcg gtccgggcgc
      121 cctaggggcc cggcggagac gggcgccgga ggtgtccgac gcctgctcgt caagttcttg
      181 ctccttggag gatatgaaga taaacaggca gcagtacgcg gagagctacg ggccgacggt
      241 gggggacagg gtgaggctgg cggacacgga cctgggggag gtggagaagg actactacta
      301 cctgggggac gaggtgaact tcgggggggg gaaggtgctg agggagggga tgggggagaa
      361 cgggacgtac acgaggacgg agaacgtgtt ggacctgctg ctgacgaacg cgctgatatt
      421 ggactacacg gggatataca aggcggacat aggggtgaag gacgggtaca tagtggggat
      481 agggaagggg gggaacccgg acataatgga cggggtgacg ccgaacatga tagtggggac
      541 ggcgacggag gtgatagcgg cggaggggaa gatagtgacg gcggggggga tagacacgca
      601 cgtgcacttc ataaacccgg accaggtgga cgtggcgctg gcgaacggga taacgacgct
      661 gttcgggggg gggacggggc cggcggaggg gagcaaggcg acgacggtga cgccggggcc
      721 gtggaacata gagaagatgc tgaagagcac ggaggggctg ccgataaacg tggggatact
      781 ggggaagggg cacgggagca gcatagcgcc gataatggag cagatagacg cgggggcggc
      841 ggggctgaag atacacgagg actggggggc gacgccggcg agcatagaca ggagcctgac
      901 ggtggcggac gaggcggacg tgcaggtggc gatacacagc gacacgctga acgaggcggg
      961 gttcttggag gacacggtga gggcgataaa cgggagggtg atacacagct tccacgtgga
     1021 gggggcgggg ggggggcacg cgccggacat aatggcgatg gcggggcacc cgaacgtgct
     1081 gccgagcagc acgaacccga cgaggccgtt cacggtgaac acgatagacg agcacttgga
     1141 catgctgatg gtgtgccacc acctgaagca gaacataccg gaggacgtgg cgttcgcgga
     1201 cagcaggata aggccggaga cgatagcggc ggaggacata ctgcacgacc tggggataat
     1261 aagcatgatg agcacggacg cgctggcgat ggggagggcg ggggagatgg tgctgaggac
     1321 gtggcagacg gcggacaaga tgaagaagca gagggggccg ctggcggagg agaagaacgg
     1381 gagcgacaac ttcaggctga agaggtacgt gagcaagtac acgataaacc cggcgatagc
     1441 gcaggggatg gcgcacgagg tggggagcat agaggagggg aagttcgcgg acctggtgct
     1501 gtgggagccg aagttcttcg gggtgaaggc ggacagggtg ataaaggggg ggataatagc
     1561 gtacgcgcag ataggggacc cgagcgcgag cataccgacg ccgcagccgg tgatggggag
     1621 gaggatgtac gggacggtgg gggacctgat acacgacacg aacataacgt tcatgagcaa
     1681 gagcagcata cagcaggggg tgccggcgaa gctggggctg aagaggagga tagggacggt
     1741 gaagaattgt cgaaacatag ggaagaagga catgaagtgg aacgacgtga cgacggacat
     1801 agacataaac ccggagacgt acgaggtgaa ggtggacggg gaggtgctga cgtgcgagcc
     1861 ggtgaaggag ctgccgatgg cgcagaggta cttcctgttc taatcgctgg gacgcccgcc
     1921 cctagaaaag gaggactaaa aatgagcaac aacaactaca tagtgccggg ggagtacagg
     1981 gtggcggagg gggagataga gataaacgcg gggagggaga agacgacgat aagggtgagc
     2041 aacacggggg acaggccgat acaggtgggg agccacatac acttcgtgga ggtgaacaag
     2101 gagctgctgt tcgacagggc ggaggggata gggaggaggc tgaacatacc gagcgggacg
     2161 gcggcgaggt tcgagccggg ggaggagatg gaggtggagc tgacggagct gggggggaac
     2221 agggaggtgt tcgggataag cgacctgacg aacgggagcg tggacaacaa ggagctgata
     2281 ttacaaaggg cgaaggagct ggggtacaag ggggtggagt aatcgctggg acgcccgccc
     2341 ctagaaaagg aggactaaaa atgcatctta atcccgccga aaaagaaaaa ttacaaatct
     2401 ttcttgcctc agaacttctt cttcgacgaa aagcccgagg ccttaaactt aattatcccg
     2461 aagccgtcgc catcatcacc tcatttatca tggaaggcgc ccgagatggc aaaaccgtcg
     2521 ccatgcttat ggaagaaggc aaacatgtcc ttacccgaga tgatgtcatg gaaggcgtcc
     2581 ccgaaatgat cgatgatatc caagccgaag ccacctttcc cgatggcacc aagttggtca
     2641 ccgtccataa tcccatctca taa
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