Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Felix Moser
Date created: 2007-12-05 12:00:00
Date modified: 2015-05-08 01:13:51
AANDENYALAA. (Very fast) SsrA degradation tag.
| Types | DnaRegion |
| Roles | Tag tag |
| Sequences | BBa_M0050_sequence (Version 1) |
Description
This sequence codes for the amino acid sequence AANDENYALAA, which when fused to the C-terminal of proteins, will make the protein susceptible to very fast degradation through SspB-mediated binding to the ClpX protease.The following rates of degradation of this tag are pulled from the corresponding references below:
~5 Vmax/ [Clpx6] min-1 from (1)
~0.5%/min on log scale from (2)
~1 min half life from (3)
See the following references for further information on degradation rates and mechanisms of this tag:
(1)McGinness, Baker, Sauer. 2006. Mol. Cell. 22:701.
(2)Flynn et al 2003. Mol. Cell. 11: 671.
Flynn et al. 2001. PNAS 98(19): 10584.
Anderson et al 1998. App. Env. Microbiol. 64(6):2240.
Notes
C-terminal tag.Degradation rate is very fast. Deviations from this sequence in key amino acids will lower degradation rates (see Parts BBa_M0051, BBa_M0052, BBa_M0053).
Three C-terminal aa's (LAA in this case) are necessary and sufficient for ClpX binding and degradation. Upstream aa sequence serves as a binding site for SspB, which guides rapid binding to ClpX.
Source
C-terminal degradation tags are commonly found in high turnover proteins in Escherichia coli.| Sequence Annotation | Location | Component / Role(s) |
| WT SsrA tag AANDENYALAA | 1,33 | feature/tag tag |